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column Product List

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Mixed-mode cation exchange solid-phase extraction column PCX series

Mixed-mode cation exchange solid-phase extraction column PCX series

Achieving the best mixed-mode solid-phase extraction for target compounds in LC-MS/HPLC analysis through optimal selection of reversed-phase and cation exchange.

The PCX series is a polymer-based mixed-mode solid-phase extraction column that combines reversed-phase and cation exchange. By selecting the optimal option from PSCX, HPSCX, and PWCX based on the strength of reversed-phase and the differences between strong cation/weak cation exchange, you can achieve highly selective solid-phase extraction of target toxins, drugs, or their metabolites. *For more details, please download the PDF or contact us.*

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Multiple Affinity Removal LC Column Human-6

Multiple Affinity Removal LC Column Human-6

Remove six types of abundant proteins with a simple method.

The "Multiple Affinity Removal Column Human6" is an LC column designed for biopharmaceutical analysis that removes six types of proteins, including abundant albumin, from human plasma samples, making it easier to separate and identify low-concentration proteins in human serum. By removing high-concentration proteins, the dynamic range of analysis can be effectively expanded. It can enhance the performance of LC/MS and electrophoresis analysis of serum samples. 【Features】 - Removes approximately 85-90% of total protein - Available in three column sizes: - 4.6 x 50mm - 4.6 x 100mm - 10 x 100mm *For more details, please refer to the related links or feel free to contact us.

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ZORBAX column / Poroshell column

ZORBAX column / Poroshell column

Dramatic improvement in productivity! Flexibility and scalability of methods for low molecular weight and biomolecule analysis.

The "ZORBAX Columns / Poroshell Columns" are specially designed products for HPLC analysis with high productivity. The silica used is of extremely high purity, very robust, and highly uniform, providing the highest level of reliability. Additionally, one of the features is the extremely wide range of phases and column configurations that cater to diverse application needs. 【Features of ZORBAX Columns】 ■ Excellent options and flexibility for low molecular weight and reversed-phase biomolecule analysis with over 18 phases, including Eclipse Plus and HILIC ■ Easy method development that demonstrates excellent performance across a wide range of target compounds and conditions ■ Improvement of UHPLC methods ■ Outstanding peak shape performance due to proven innovative silica and bonding technology ■ Long-term reliability by having the necessary options available when needed for analysis and preparative purification *For more details, please refer to the PDF materials or feel free to contact us.

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ACQUITY UPLC column

ACQUITY UPLC column

The separation energy, sensitivity, and speed have dramatically improved.

With UPLC technology, you can invest in the future by having the robustness and reliability to perform existing HPLC analytical methods within a single system, while optimally operating UPLC columns with particle sizes of 2 μm or less. The ACQUITY UPLC(R) system family serves as a platform for LC technology that can be utilized regardless of separation requirements, making it easy to enhance productivity and facilitate method transfer between facilities. 【Features】 - Six types of UPLC particles based on ethylene-bridged hybrid, High Strength Silica, and Charged Surface Hybrid - 22 types of stationary phases - Seven types of application-specific chemistries - Designed to seamlessly deliver performance with the ultra-low dispersion ACQUITY UPLC system - Over 200 options (column sizes and chemistries) available to select the optimal column that balances separation capability and throughput For more details, please contact us or download the catalog. *The catalog will be a digest version. If you need the full version, please contact us.

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mRP-C18 High Recovery Protein Column

mRP-C18 High Recovery Protein Column

Silica filled with porous C18 bonded ultra-pure 5μm particles that reduce or remove protein adsorption!

We would like to introduce our bio-pharmaceutical analysis LC column, the 'mRP-C18 High Recovery Protein Column'. This column and the desalting LC column can separate complex protein samples (e.g., immunodepleted serum or plasma proteins) into the number of fractions selected by the user, as well as perform desalting of the samples. It achieves significantly better recovery rates for protein samples compared to conventional RP HPLC columns. Please feel free to consult us if you have any requests. 【Features】 ■ Load up to a total of 380μg of protein ■ Available in two column sizes (0.5×100mm, 4.6×50mm) ■ Maximum operating pressure is 250bar (4000psi) ■ Compatible with water and all common organic solvents ■ Confirmed 95-99% protein sample recovery rate using immunodepleted serum with a multiple affinity removal system LC column *For more details, please refer to the related links or feel free to contact us.

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UHPLC column

UHPLC column

Equivalent selectivity to existing ZORBAX columns! Optimize separation by changing the stationary phase!

This product is a UHPLC column that achieves a maximum operating pressure of 120 MPa. When combined with the Agilent 1290 Infinity LC System, it delivers the highest level of performance. It is suitable for continuous analysis with durability and stable reproducibility, making it compatible with high-pressure UHPLC systems from various manufacturers. 【Features】 ■ Achieves a maximum operating pressure of 120 MPa through optimization of hardware and packing processes ■ Delivers the highest level of performance when combined with the Agilent 1290 Infinity LC System ■ Equivalent selectivity to existing ZORBAX columns ■ Durability and stable reproducibility suitable for continuous analysis ■ Compatible with high-pressure UHPLC systems from various manufacturers *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column for optical isomer separation

HPLC column for optical isomer separation

HPLC column for optical isomer separation

Optical isomers present in various bioactive substances (such as pharmaceuticals, pesticides, and fragrances) that are deeply related to our daily lives are known to exhibit significantly different biological activities between isomers, increasing the necessity for the analysis and separation of optical isomers. The "SUMICHIRAL OA series" HPLC columns for the separation of optical isomers are gaining attention in fields such as pharmaceuticals and pesticides.

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UHPLC Emitter Column "Aurora Series"

UHPLC Emitter Column "Aurora Series"

Robust column design. It can reduce loading and balancing time while increasing the identification count.

The "Aurora Series" is a UHPLC emitter column designed and manufactured to eliminate all dead volume at the column inlet and outlet. It maximizes the sample separation capability of chromatography packing materials. Additionally, it can be connected without the need for cumbersome and time-consuming adapters for fittings. 【Features】 ■ Reduces loading and equilibration time while increasing the number of identifications ■ Achieves excellent reproducibility ■ Robust column design ■ Enables maximum peptide identification *For more details, please refer to the PDF document or feel free to contact us.

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Nano UHPLC column "Aurora Generation 3"

Nano UHPLC column "Aurora Generation 3"

Improved separation energy, throughput, and reproducibility with the nanoZero and emitter-integrated structure achieving zero dead volume.

"Aurora Generation 3" is a nano UHPLC column that can bind more peptides, allowing for excellent identification results with high separation capability even with a smaller sample volume. Additionally, the unique "nanoZero" design eliminates dead volume and achieves high pressure resistance of over 1,700 bar. The emitter-integrated column design provides improved longevity and spray stability, even between different columns and manufacturing batches, resulting in enhanced column lifespan. 【Features】 ■ Improved separation capability with the newly adopted 1.7μm particle size C18 stationary phase ■ Increased pressure resistance due to improvements in nanoZero fittings ■ Enhanced spray stability and column lifespan through improvements in the emitter-integrated design *For more details, please download the PDF or feel free to contact us.

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Fast Guard Column for Bioanalysis

Fast Guard Column for Bioanalysis

Available for use with AdvanceBio Glycan Mapping Columns (2.7μm, surface porous, pressure resistant up to 600 bar)!

We would like to introduce the "Fast Guard Column for Bioanalysis" that we handle. The "Agilent Bio UHPLC Guard" is a high-performance guard column designed for Agilent's high-speed LC column family, using easy-to-install hardware that fits directly at the end of the column. By using the Fast Guard for UHPLC, you can extend the lifespan of the analytical column without compromising performance. 【Features】 - No special hardware required, just connect to the column - Comes in a package of three - Usable with AdvanceBio Peptide Mapping Columns (pressure tolerance 600 bar) - Usable with AdvanceBio Glycan Mapping Columns (1.8μm, pressure tolerance 1200 bar) - Extends the lifespan of high-speed LC analytical columns without degrading performance *For more details, please refer to the related links or feel free to contact us.

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Column "ZORBAX RRHD - Wide Pore"

Column "ZORBAX RRHD - Wide Pore"

Achieving excellent separation capability with 1.8μm particles in most defined separations!

The "ZORBAX RRHD-Wide Pore" is an LC column suitable for the analysis of biopharmaceuticals, providing highly reproducible separations of proteins and peptides. To efficiently separate proteins, peptides, or other macromolecules, the sample needs to be in close proximity to the stationary phase surface, which is why such a wide pore (300Å) column is necessary. Four different stationary phases (C18/C8/C3/Diphenyl) are available, allowing for the optimization of selectivity and recovery rates for proteins and polypeptides. 【Features】 ■ High-pressure (1200 bar) column for obtaining suitable results with the 1290 Infinity LC or other UHPLC systems ■ Usable with HILIC Plus ■ The selectivity remains the same for 3.5μm and 5μm ZORBAX columns with the same stationary phase, ensuring compatibility with various LCs *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX RRHD column

ZORBAX RRHD column

A new level of sensitivity and separation capability! It aligns many binding phases and flexibly responds to various analyses.

The 'ZORBAX RRHD Column' we handle achieves true high-resolution analysis without compromising stability. Thanks to its excellent flexibility, it is possible to increase the flow rate to the column's limit without reducing separation efficiency or quality. With more than 12 types of bonded phases available, including HILIC, you can select the appropriate column according to your analytical needs. 【Features】 ■ Stability up to 1200 bar: Stable use with UHPLC equipment is possible. ■ Separation capability: Stable high-resolution analysis and scalability are achieved with 1.8 μm particles, making method conversion with 3.5 μm and 5 μm particles easy. ■ More reliable compound identification: High-resolution chromatograms and a column with an inner diameter of 2.1 mm enhance MS separation capability. ■ A wide range of selectable bonded phases: By combining ZORBAX and Poroshell 120 families, it becomes easier to accommodate various samples and facilitate method conversion. *For more details, please refer to the PDF document or feel free to contact us.

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[Application Example] HPLC Analysis of Sugars Using the CarboSep Series

[Application Example] HPLC Analysis of Sugars Using the CarboSep Series

You can choose the column that suits you from a rich lineup.

Concise Separations, Inc. in the United States has been manufacturing and selling HPLC products for over 20 years. The CarboSep series supports various sugar analyses and is used by customers around the world. We have also included actual analysis examples in the case studies, so please take a look.

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HPLC column for organic acid analysis - 'Coregel Series'

HPLC column for organic acid analysis - 'Coregel Series'

You can choose from a rich lineup of columns for organic acid analysis.

Concise Separations, Inc. (formerly Transgenomic, Inc.) has been providing various HPLC columns for over 20 years. We offer the Coregel series as columns for organic acid analysis.

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Guard Column "OPTI-GUARD 1MM & 3MM"

Guard Column "OPTI-GUARD 1MM & 3MM"

Directly connected to the top of the analysis column! Introducing the guard column manufactured by OPTIMIZE.

"OPTI-GUARD 1MM&3MM" is a guard column that can be used for analytical columns (inner diameters of 4.6mm and 3.0mm) as well as for narrow-bore columns (inner diameters of 2.1mm and 1.0mm) that are commonly used with LCMS. It connects directly to the top of the analytical column. The lifetime is up to 1,000 injections. Additionally, we also offer the "EXP GUARD COLUMNS," a trap column for UHPLC that can withstand pressures up to 20,000 psi. 【Features of OPTI-GUARD 1MM&3MM】 ■ Directly connects to the top of the analytical column ■ The 1mm type does not interfere with the separation of the analytical column currently in use by the customer ■ Can be used as a common guard column for various separation columns from different manufacturers ■ Lifetime is up to 1,000 injections *For more details, please refer to the PDF document or feel free to contact us.

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Technical Information about TSKgel UP-SW3000

Technical Information about TSKgel UP-SW3000

High-performance SEC column TSKgel UP-SW3000 with a particle diameter of 2μm is now available for sale!

The latest column is UP-SW3000-LS. Please refer to that as well. This document is a technical material that explains the high-performance SEC column TSKgel UP-SW3000. We will introduce the basic characteristics and examples of separation. [Contents (excerpt)] ■ Introduction ■ Basic characteristics of TSKgel UP-SW3000 ■ Application examples - Precise analysis of antibody drugs - Separation example of antibody papain digests ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

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HPLC Column "Reversed Phase / HILIC"

HPLC Column "Reversed Phase / HILIC"

Numerous charts are included. In addition to the characteristics of each mode, we introduce the main target substances.

This document contains information on HPLC columns for normal phase chromatography and multi-mode chromatography. In addition to the characteristics of each mode, it introduces the main target substances. Furthermore, it includes charts and tables for separation and comparison. [Contents (excerpt)] ■ Normal Phase Chromatography ■ Multi-Mode Chromatography ■ Columns for LC/MS ■ Reverse Phase Chromatography *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column "IEC・HIC・Affinity"

HPLC column "IEC・HIC・Affinity"

This is a book that explains the features and physical properties of ion exchange chromatography and other related topics.

This document explains the IEC, HIC, and affinity of HPLC columns. It introduces the characteristics of ion exchange chromatography columns, as well as basic physical properties. Additionally, it explains each product series using tables. Furthermore, it also includes information on hydrophobic chromatography and affinity chromatography. 【Contents (excerpt)】 ■ Ion Exchange Chromatography ■ Hydrophobic Chromatography ■ Affinity Chromatography *For more details, please refer to the PDF document or feel free to contact us.

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HPLC/UHPLC column "FlexFire C18"

HPLC/UHPLC column "FlexFire C18"

Making HPLC⇔UHPLC more accessible! This is a column that uses refined silica gel.

The "FlexFire C18" is a highly versatile HPLC/UHPLC column that emphasizes hydrophobic interactions due to high carbon content. It provides results that are faithful to the fundamentals of HPLC (UHPLC) and offers important information to establish future directions. It allows for easy setting of analytical conditions, as it can be transferred between quality control and research & development according to the system. 【Features】 ■ Extremely versatile ■ Emphasizes hydrophobic interactions due to high carbon content ■ Available in 5μm, 2.6μm, and 1.6μm ■ Transferable between quality control and research & development according to the system ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX RRHD 300 HILIC"

Column "ZORBAX RRHD 300 HILIC"

The HILIC function is specially designed for the separation of glycopeptide variants!

The "ZORBAX RRHD 300 HILIC" is a column for the analysis of glycopeptides, featuring a particle size of 1.8μm and a pore size of 300Å. It is designed to provide consistent and outstanding performance for the separation and characterization of peptides, proteins, antibodies, complexes, novel biomaterials, and biopharmaceuticals. The technology of AdvanceBio columns enhances accuracy and productivity, allowing for more information to be obtained in various characterization analyses. 【Features】 ■ Excellent peak shape for polar glycopeptides - These polar compounds typically cannot be separated using reverse phase columns. ■ High-speed analysis and stability up to 1200 bar - With a particle size of 1.8μm, it enables fast separation using UHPLC without compromising data accuracy. *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column "Ion Chromatography"

HPLC column "Ion Chromatography"

Explanation of separation and analysis using anion analysis columns, illustrated with diagrams.

This document explains ion chromatography of HPLC columns. It provides an explanation of the separation and analysis of both anion analysis columns and cation analysis columns, using diagrams and tables. 【Contents (excerpt)】 ■ Anion analysis column (for IC-2010) ■ Cation analysis column (for IC-2010) ■ Anion analysis column (non-suppressor type) ■ Anion analysis eluent ■ Cation analysis column (non-suppressor type) *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column "Size Exclusion Chromatography"

HPLC column "Size Exclusion Chromatography"

Features of the SEC column and a list of compatibility with H-type solvents are included.

This document explains size exclusion chromatography (SEC) for HPLC columns. It includes features of SEC columns and a list of compatible H-type solvents. Additionally, the section on SEC column selection provides a detailed explanation of both aqueous SEC column selection (for synthetic polymers) and organic solvent-based SEC column selection, using tables for clarity. 【Contents (excerpt)】 ■ Features of SEC columns ■ List of compatible H-type solvents ■ SEC column selection ■ Aqueous SEC columns (for proteins, peptides, and nucleic acids) ■ Aqueous SEC columns (for synthetic polymers, polysaccharides, oligosaccharides, and nucleic acids) *For more details, please refer to the PDF document or feel free to contact us.

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HPLC/UHPLC column "FlexFire mAb-RP"

HPLC/UHPLC column "FlexFire mAb-RP"

From intact protein to post-digestion peptide analysis! This single column completes it all.

"FlexFire mAb-RP" is a column developed for HPLC/UHPLC using 1000Å silica gel as the substrate. This single column can complete the analysis of macromolecules such as intact proteins and monoclonal antibodies, as well as digested peptides. By matching the molecular weight of the compounds with the pore size of the silica gel substrate, it enables more sensitive analysis. [Features] - Developed using 1000Å silica gel as the substrate - Supports analysis from intact proteins to digested peptides - Enables more sensitive analysis by matching the molecular weight of the compounds with the pore size of the silica gel substrate *For more details, please refer to the PDF document or feel free to contact us.

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Core-shell type filling column "SunShell"

Core-shell type filling column "SunShell"

Column back pressure halved, achieving high separation! A column that also achieves high durability.

"SunShell" is a core-shell type filling column that achieves efficient silanol group deactivation and high durability by using the improved Sunniest-bonding method for core-shell silica. The core-shell column exhibits equivalent performance (theoretical plates) at half the back pressure of a column with a particle size of 1.8µm. Due to the low back pressure, there are fewer failures in UHPLC even after prolonged use. 【Features】 - Core sizes of 1.6μm, 3.0μm, and 3.4μm with porous layers of 0.5μm, 0.2μm, and 0.6μm - 2.6μm offers the same number of plates as fully porous Sub2μm and 2μm, with the same column pressure as fully porous 3μm - 5μm has the same number of plates as fully porous 3μm and the same column pressure as fully porous 5μm - 3.4μm is a core-shell packing material for rapid protein separation - The reverse phase stationary phases such as C18 and C8 have the same surface treatment as Sunniest For more details, please contact us or download the catalog.

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HPLC/UHPLC column "FlexFire C1"

HPLC/UHPLC column "FlexFire C1"

It is possible to shorten analysis time! It has surprising performance that enhances the retention of highly polar compounds to the maximum limit.

The first choice of columns in HPLC/UHPLC method development is almost always C18, and to change selectivity from C18, columns such as C30, C8, and HILIC are selected as second choices. The 'FlexFire C1' is a column that uses refined silica gel. The perception of "this product = low adsorption" holds unexpected performance that enhances peak shape and peak sensitivity, as well as maximizes the retention of highly polar compounds. 【Features】 ■ Contains unexpected performance that maximizes the retention of highly polar compounds ■ Significantly weakens excessively strong retention ■ Allows for reduced analysis time without changing the method ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

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[Case Study] AAV Titer Measurement After Downstream Process

[Case Study] AAV Titer Measurement After Downstream Process

By adopting size exclusion chromatography and highly sensitive fluorescence detection, accurate and reproducible measurements can be achieved with a small amount of sample!

We introduce a rapid and cost-effective method for measuring the titer of adeno-associated virus (AAV) products after downstream processing, combining size exclusion chromatography (SEC) with a highly sensitive fluorescence detector. By using the Thermo Scientific MAbPac SEC-1 column, efficient separation of AAV capsids and low molecular weight impurities is achieved, allowing for detailed monitoring of product quality attributes (PQA). This method enables rapid determination of AAV product titer after a single step of affinity purification, contributing to the evaluation of product characteristics. 【Features】 ■ Rapid and cost-effective titer measurement method ■ Measurement possible with small sample sizes due to high-sensitivity fluorescence detection ■ Efficient separation using the MAbPac SEC-1 column ■ Detailed monitoring of PQA is possible ■ Improved reproducibility with low-adsorption glass vials (RSD less than 5%) *For more details, please download the PDF or feel free to contact us.

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FlexFire SEC Series Columns for HPLC/UHPLC

FlexFire SEC Series Columns for HPLC/UHPLC

Easily analyze polymer compounds! Methods can also be easily constructed.

The "FlexFire SEC Series" is a column designed for the separation of macromolecules such as proteins and antibodies, as well as low molecular weight compounds, based on their size in solution. This product primarily utilizes isocratic elution, and methods can be easily constructed. Additionally, the results obtained are straightforward, making it suitable for the analysis of intact biomacromolecules such as proteins and antibodies. 【Features】 ■ Easy analysis of macromolecules ■ Separation based on size ■ Primarily uses isocratic elution, with easy method construction ■ Simple results obtained ■ Well-suited for the analysis of intact biomacromolecules such as proteins and antibodies *For more details, please refer to the PDF document or feel free to contact us.

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TSKgel UP-SW Series

TSKgel UP-SW Series

Microparticle filler! Rapid analysis of biomacromolecules (15cm) and high-resolution analysis (30cm) are possible.

The "TSKgel UP-SW series" is a size exclusion chromatography that is compatible with both UHPLC and HPLC systems. It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. The surface characteristics of the packing material are the same as the existing "TSKgel SW type." Additionally, we also offer the "TSKgel SuperSW mAb series," "UltraSW Aggregate," and "TSKgel SuperSW, SWXL, SW, BioAssistSWXL series." 【Features】 - Due to the use of fine particle packing materials (2μm/3μm), rapid analysis (15cm) and high-resolution analysis (30cm) of biomacromolecules are possible. - The surface characteristics of the packing material are the same as the existing TSKgel SW type. - Compatible with both UHPLC and HPLC systems. - It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. *For more details, please refer to the PDF document or feel free to contact us.

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HPLC/UHPLC column "FlexFire C30"

HPLC/UHPLC column "FlexFire C30"

Dramatically updated! Not just a second choice, but also active as a main column.

The "FlexFire C30" was introduced in 1998 and has been a representative column used by many people to this day. Updates include an expanded pH range, improved pressure resistance, and enhanced retention and separation of highly polar compounds. Analysis with a 100% aqueous mobile phase can still be set up as before. It serves not just as a second choice, but also as a main column. 【Features】 ■ Expanded pH range ■ Improved pressure resistance ■ Enhanced retention and separation of highly polar compounds ■ Serves as a main column, not just a second choice ■ Utilizes refined silica gel *For more details, please refer to the PDF materials or feel free to contact us.

  • Other protein analysis
  • Column Chromatography
  • column

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SEC column "TSKgel(R) UP-SW3000-LS"

SEC column "TSKgel(R) UP-SW3000-LS"

Reduced initial protein adsorption! Compatible with both UHPLC and HPLC systems.

The "TSKgel(R) UP-SW3000-LS" is a size exclusion chromatography column for the separation of biomacromolecules, using a filler with a particle size of 2μm that has diol groups chemically bonded to the surface of silica gel. Due to the use of fine particle fillers (2μm), it enables rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins (antibodies, enzymes). It has minimal column shedding, making it suitable for measurements using light scattering detectors. 【Features】 ■ The surface characteristics of the filler are similar to the existing TSKgel SW type. ■ Minimal column shedding makes it ideal for measurements using light scattering detectors. ■ Improved filler and column materials reduce initial protein adsorption. ■ Shipping and storage solvents are easily managed with a 20% ethanol aqueous solution. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

  • Other physicochemical equipment
  • column

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