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column Product List

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TSKgel UP-SW Series

TSKgel UP-SW Series

Microparticle filler! Rapid analysis of biomacromolecules (15cm) and high-resolution analysis (30cm) are possible.

The "TSKgel UP-SW series" is a size exclusion chromatography that is compatible with both UHPLC and HPLC systems. It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. The surface characteristics of the packing material are the same as the existing "TSKgel SW type." Additionally, we also offer the "TSKgel SuperSW mAb series," "UltraSW Aggregate," and "TSKgel SuperSW, SWXL, SW, BioAssistSWXL series." 【Features】 - Due to the use of fine particle packing materials (2μm/3μm), rapid analysis (15cm) and high-resolution analysis (30cm) of biomacromolecules are possible. - The surface characteristics of the packing material are the same as the existing TSKgel SW type. - Compatible with both UHPLC and HPLC systems. - It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. *For more details, please refer to the PDF document or feel free to contact us.

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HPLC/UHPLC column "FlexFire C30"

HPLC/UHPLC column "FlexFire C30"

Dramatically updated! Not just a second choice, but also active as a main column.

The "FlexFire C30" was introduced in 1998 and has been a representative column used by many people to this day. Updates include an expanded pH range, improved pressure resistance, and enhanced retention and separation of highly polar compounds. Analysis with a 100% aqueous mobile phase can still be set up as before. It serves not just as a second choice, but also as a main column. 【Features】 ■ Expanded pH range ■ Improved pressure resistance ■ Enhanced retention and separation of highly polar compounds ■ Serves as a main column, not just a second choice ■ Utilizes refined silica gel *For more details, please refer to the PDF materials or feel free to contact us.

  • Other protein analysis
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SEC column "TSKgel(R) UP-SW3000-LS"

SEC column "TSKgel(R) UP-SW3000-LS"

Reduced initial protein adsorption! Compatible with both UHPLC and HPLC systems.

The "TSKgel(R) UP-SW3000-LS" is a size exclusion chromatography column for the separation of biomacromolecules, using a filler with a particle size of 2μm that has diol groups chemically bonded to the surface of silica gel. Due to the use of fine particle fillers (2μm), it enables rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins (antibodies, enzymes). It has minimal column shedding, making it suitable for measurements using light scattering detectors. 【Features】 ■ The surface characteristics of the filler are similar to the existing TSKgel SW type. ■ Minimal column shedding makes it ideal for measurements using light scattering detectors. ■ Improved filler and column materials reduce initial protein adsorption. ■ Shipping and storage solvents are easily managed with a 20% ethanol aqueous solution. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

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HPLC/UHPLC column "FlexFire AQ C18"

HPLC/UHPLC column "FlexFire AQ C18"

Retention and separation of highly polar compounds in reverse phase systems! It can be handled just like before.

The "FlexFire AQ C18" is a column that can perform well across a wide range in both first and second applications. In reversed-phase columns, retaining and separating highly polar compounds can be extremely challenging. As a result, it is often the case that HILIC columns are chosen, but this column has been developed as an additional option before transitioning to HILIC columns. The frequently used C18 group in familiar reversed-phase columns has no change in usage, allowing for handling in the same manner as before. 【Features】 ■ Suitable for retaining and separating highly polar compounds in reversed-phase systems ■ Can perform well across a wide range in both first and second applications ■ Serves as an additional option before transitioning to HILIC columns ■ Can be handled in the same manner as before ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
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UHPLC column

UHPLC column

Improve productivity! A lineup of columns that maximizes the performance of UHPLC systems.

Many of the LC columns currently available on the market cannot fully leverage the benefits derived from the performance of the latest UHPLC systems. Waters offers a variety of columns for UHPLC, such as CORTECS columns and eXtended Performance [XP] columns, to assist customers in overcoming challenges in their analyses. This expands the options for column selection and maximizes the potential of analytical laboratories. **Features** - CORTECS 2.7μm columns that utilize solid-core (core-shell) particles to maximize UHPLC separation performance - XP columns that use three types of particle substrates: ethylene-bridged hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH), providing excellent selectivity and flexibility in method development - Column sizes and particle diameters designed for UHPLC - Seamless method transfer between UPLC, UHPLC, and HPLC - Exceptional reproducibility between batches and columns through comprehensive management of the entire manufacturing process from silica and hybrid synthesis to the final product

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SEC column "TSKgel UP-SW Aggregate"

SEC column "TSKgel UP-SW Aggregate"

Rapid analysis (15 cm column) and high-resolution analysis (30 cm) of biomacromolecules such as antibody aggregates, high molecular weight proteins, and nucleic acid fragments are possible.

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● Ideal for the separation of high molecular weight aggregates of antibodies, high molecular weight proteins, and nucleic acid fragments. ■ Quick analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

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SEC column "TSKgel UP-SW2000"

SEC column "TSKgel UP-SW2000"

Due to the microparticle filler (2 μm), rapid analysis of biomacromolecules and mid-sized molecules such as low molecular weight proteins, peptides, and oligonucleotides is possible.

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● It is suitable for the separation of low molecular weight proteins, fragments, peptides, and oligonucleotides. 【Features】 ■ Rapid analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.*

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Technical Data: The purification of bispecific antibodies is settled with this! (2)

Technical Data: The purification of bispecific antibodies is settled with this! (2)

Selection of chromatography suitable for the separation of aggregated impurities related to antibodies.

During the production of bispecific antibodies (BsAb), various impurities such as mispaired antibodies, aggregates, incomplete antibodies, and fragment antibodies are often generated. Therefore, in the downstream purification process of BsAb, it is important to effectively separate and remove these impurities derived from various products and process-related impurities using chromatography. This report presents the separation modes of chromatography used in the isolation and purification of BsAb that have been previously reported, along with the corresponding packing materials and separation conditions.

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Tosoh's SEC columns for biomacromolecules "UP-SW Series"

Tosoh's SEC columns for biomacromolecules "UP-SW Series"

The "UP-SW Series" SEC columns for biomacromolecules from Tosoh, known for their proven performance and reliability. These are high-resolution SEC columns with minimal non-specific adsorption.

Optimized microparticle fillers (2µm, 3µm) enable rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins, antibodies, and enzymes. The surface characteristics of the fillers are similar to those of the widely used general-purpose SWXL series columns. They are compatible with both UHPLC and HPLC systems.

  • UPSWシリーズ.png
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Bond Elut LMS

Bond Elut LMS

Bond Elut LMS elutes target compounds without the addition of amines, buffers, or acids.

Bond Elut LMS elutes target compounds without the addition of amines, buffers, or acids. The polymer, which eliminates secondary interactions, means that elution of compounds can be achieved with organic solvents or low ionic strength HPLC mobile phase solvents. This feature ensures compatibility with precision analytical techniques such as LC/MS. Bond Elut LMS is characterized by an optimized particle size and a large surface area that enhances extraction efficiency, without interfering with clear extraction. Bond Elut LMS ensures excellent recovery rates and reproducibility, reducing downtime and ensuring trouble-free operation. *For more details, please refer to the PDF materials or feel free to contact us.*

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Column "AdvanceBio Amino Acid Analysis (AAA)"

Column "AdvanceBio Amino Acid Analysis (AAA)"

Designed as an innovative solution for the characterization of biomolecules!

The "AdvanceBio Amino Acid Analysis (AAA)" is an LC column for biopharmaceutical analysis that can separate amino acids from protein hydrolysates and cell culture media with high speed, excellent sensitivity, and reproducibility. It includes proven reagents for amino acid derivatization, a prepared amino acid standard kit, columns based on our innovative Poroshell technology, and support from our experts. When used in conjunction with Agilent InfinityLab LC series instruments, it provides a complete solution for amino acid analysis. 【Features】 ■ Achieves high separation capability with the efficient particle morphology of Poroshell ■ Long column life due to robust chemically modified silica with excellent resistance to high pH ■ Utilizes 2.7μm diameter particles, compatible with both HPLC and UHPLC systems ■ Standard solutions and reagents available as a kit ■ Uses a general analysis injection system *For more details, please refer to the PDF document or feel free to contact us.

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SEC column "TSKgel UP-SW3000"

SEC column "TSKgel UP-SW3000"

Due to the microparticle filler, rapid analysis of biomacromolecules such as proteins, antibodies, and enzymes is possible.

The latest column is the UP-SW3000-LS. Please refer to that as well. The "TSKgel UP-SW3000" is a size exclusion chromatography column for the separation of biomacromolecules, using a packing material that chemically bonds diol groups to the surface of silica gel particles with a diameter of 2μm. Due to the fine particle packing material, rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins, antibodies, and enzymes are possible. 【Features】 ■ Enables rapid analysis (15cm column) and high-resolution analysis (30cm) ■ Surface characteristics of the packing material are similar to existing TSKgel SW types ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments ■ Compatible with both UHPLC and HPLC systems *For more details, please refer to the PDF document or feel free to contact us.

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Simple and efficient mixed-mode solid-phase extraction column OFX from saliva samples.

Simple and efficient mixed-mode solid-phase extraction column OFX from saliva samples.

By specializing in saliva collection kits, it is possible to efficiently extract target compounds from saliva mucosa and additives, making it ideal for TDM, drug testing, and metabolite analysis.

The OFX series specializes in solid-phase extraction using saliva as the starting sample, making it optimal for drug testing and metabolite analysis from saliva using LC-MS or HPLC. The polymer-based mixed-mode solid-phase extraction columns are very effective for sample pretreatment, including desalting, removal of oral mucins, and concentration of target compounds. *For more details, please download the PDF or contact us.

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  • Dispenser
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Multiple Affinity Removal Column - Human7

Multiple Affinity Removal Column - Human7

Remove seven types of excess protein with a simple method.

The "Multiple Affinity Removal Column Human7" is an LC column designed for biopharmaceutical analysis that removes seven types of proteins, including abundant albumin, from human plasma samples, making it easier to separate and identify low-concentration proteins in human serum. By removing high-concentration proteins, the dynamic range of analysis can be effectively expanded. It can enhance the performance of LC/MS and electrophoresis analysis of serum samples. 【Features】 ■ Removes approximately 88-92% of total protein ■ Available in three column sizes: ・4.6 x 50mm ・4.6 x 100mm ・10 x 100mm ■ Custom-made column sizes are also available *For more details, please refer to the related links or feel free to contact us.

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Chromolith　coated　with　ＯＡ−５０００

Chromolith　coated　with　ＯＡ−５０００

Chromolith　coated　with　ＯＡ−５０００

The "Monolithic ODS Column Chromolith RP-18" manufactured by MERCK is a ligand-exchange type column for optical isomer separation, coated with the chiral selector SUMICHIRAL OA-5000. Monolithic HPLC columns use a cylindrical silica continuum instead of a 3-5 μm silica gel support. This silica continuum consists of a structure of macropores (approximately 2 μm) and mesopores (approximately 13 nm), with a high porosity of about 80%. By using this monolithic column as the substrate for the stationary phase of a chiral column, excellent characteristics can be achieved.

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Reverse-phase chiral column ULTRON ES series protein stationary phase

Reverse-phase chiral column ULTRON ES series protein stationary phase

To everyone seeking chiral analysis under reverse phase conditions!

ULTRON ES-OVM is designated as an evaluation column for Clopidogrel bisulfate and Escitalopram oxalate in the United States Pharmacopeia (USP). It also corresponds to the ovomucoid chemically bonded amino silica gel described in the Japanese Pharmacopoeia (JP). ■ULTRON ES-OVM (USP L57) It uses ovomucoid, which is resistant to denaturation, as the ligand. 〈Product Lineup〉 ULTRON ES-OVM ULTRON ES-OVM-3 can be used for UHPLC and LC/MS ULTRON ES-OVM-C for shipping inspection with Clopidogrel bisulfate (USP compliant) ULTRON ES-OVM-E for shipping inspection with Escitalopram oxalate (USP compliant) ■ULTRON ES-PEPSIN It uses pepsin, a polypeptide-degrading enzyme, as the ligand. It is effective for the chiral separation of β-blockers.

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