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column Product List

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Column for high-performance liquid chromatography

A rich lineup of 38 types, capable of direct separation of optical isomers.

We manufacture and sell world-leading HPLC columns. The separation in high-performance liquid chromatography is greatly influenced by the characteristics of the column. Utilizing advanced technology and years of accumulated know-how, we support our customers from analysis to fractionation. The optical isomer separation column "SUMICHIRAL OA Series" has become an essential HPLC column in the pharmaceutical and pesticide fields. 【Features】 - Direct separation of optical isomers is possible, achieving excellent separation. - By changing the absolute configuration of the chiral selector in the column, the elution order of isomers can be reversed (excluding some columns). - The chiral selector is chemically stable as it is covalently bonded to silica gel (excluding some columns). We have launched a new product, 'OA-5000'. 【Features】 - The theoretical plate number has improved to 1.5 times that of conventional products (equivalent to a particle-filled column with a particle size of 3.5μm). - The column pressure is less than one-third of that of conventional products, allowing for increased flow rates during measurement. - Columns can be connected up to approximately 45 cm. - Ideal for analyzing samples with strong retention (such as dicarboxylic acids). *For details, please refer to "Request for Materials" or "Catalog Download."

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Chiral chromatography

A comprehensive lineup that caters to any analysis scene! Featuring products like "ChiraSelect."

This document introduces "Chiral Chromatography." It features the "CYCLOBOND" column series, which has excellent chiral recognition capabilities, as well as the unique "CLC" column that utilizes chelation with copper ions, and the classic series of cyclodextrin-based columns "α, β, γ-DEX." The explanations are made clear using diagrams and graphs. Please feel free to download and take a look. 【Contents (excerpt)】 ■ HPLC Columns ・CHIROBIOTIC ・CYCLOBOND ・CLC ・ChromegaChiral, GreenSep *For more details, please refer to the PDF document or feel free to contact us.

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VariTide RPC

Developing a polymer-based HPLC column to economically enhance throughput and analyze and purify synthetic peptides!

We would like to introduce our LC column for biopharmaceutical analysis, 'VariTide RPC.' To efficiently retain peptides composed of 5 to 60 amino acids, we adjust the particle size and pore size. It can be used at various pH ranges, allowing for the separation and purification of acidic, basic, hydrophilic, and hydrophobic peptides with this product. 【Features (Specifications)】 ■ Polymer: Macroporous spherical styrene-divinylbenzene ■ Particle size: 6μm ■ Pore diameter: 200Å ■ Functional groups: None ■ Column size: Inner diameter: 4.6mm to 21.2mm / Length: 25cm *For more details, please refer to the related links or feel free to contact us.

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Column "MicroBore"

When the sample mass is the same, the detection limit is improved by five times compared to a column with an inner diameter of 2.1 mm!

The "MicroBore" is a column suitable for the analysis of trace samples. This product uses low flow rates (generally below 50 μL/min). Therefore, it is suitable for use with detectors that require low flow rates, such as some mass spectrometers. The guard column can be adjusted for the depth of the tube stop, allowing for a complete zero dead volume connection at all times. 【Features】 ■ Suitable for use in HPLC systems purchased or modified for trace analysis ■ Achieves high sensitivity even with small sample volumes ■ Compatibility with LC/MS interfaces ■ A wide variety of stationary phases available ■ Guard columns that can be used with MicroBore columns (inner diameter 1.0 mm) and capillary columns *For more details, please refer to the related links or feel free to contact us.

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Column "Ultron ES"

Acetonitrile, ethanol, and phosphate buffer can be used as mobile phases!

"Ultron ES" is an insolubilized protein column equipped with asymmetric recognition sites for the optical isomer separation of numerous chiral compounds. It is designed to have a chiral stationary phase with two complementary protein bases, making it suitable for HPLC separation of optical isomers without derivatization. Additionally, it is suitable for the separation of pharmaceuticals. It can directly separate racemates without going through derivatization. 【Features】 ■ Ultron ES-OVM is used for the separation of optical isomers of acidic and basic pharmaceuticals. ■ Basic compounds that are difficult to separate with common products can sometimes be easily separated. ■ The ES-OVM column and ES-Pepsin column use a 120Å, 5μm silica packing that combines ovomucoid protein and pepsin protein. *For more details, please refer to the related links or feel free to contact us.

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Column "ZORBAX 300Extend-C18"

High robustness and separation of polypeptides and peptides over a wide range of pH from high pH to low pH (pH 2 to 11.5)!

The "ZORBAX 300Extend-C18" is a wide-bore HPLC column that efficiently separates peptides in the pH range of 2 to 11.5. Its unique bidentate bonding phase provides excellent longevity and reproducibility in both high and low pH regions. The retention characteristics and selectivity for peptides and polypeptides in the high pH region significantly change as these molecules become charged. 【Features】 ■ Shows different selectivity at high and low pH ■ Achieves excellent efficiency and good recovery rates for hydrophobic peptides at high pH ■ Suitable for LC/MS analysis using ammonium hydroxide mobile phase *For more details, please refer to the PDF document or feel free to contact us.

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Raptor LC Column

A variety of stationary phases and particle sizes! LC columns with better selectivity that improve separation efficiency.

Restek's "Raptor LC Column" is a highly efficient LC column that significantly reduces analysis time. It includes options suitable for biological sample analysis, such as the "Raptor Biphenyl Column" for drug and metabolite analysis, as well as the "Raptor ARC-18 Column." It offers better selectivity that improves resolution and sustainable reproducibility. 【Features】 ■ High efficiency that significantly reduces analysis time ■ Better selectivity that improves resolution ■ Improved sample throughput with existing HPLC and UHPLC ■ Sustainable reproducibility ■ A variety of stationary phases and particle sizes *For more details, please refer to the PDF document or feel free to contact us.

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Column "Hi-Plex"

High efficiency and low operating pressure! Separation of carbohydrates and alcohols is possible through ligand exchange.

"Hi-Plex" is a column that achieves high efficiency, low operating pressure, and long column life due to its monodisperse materials. With a series of ligand-to-ion pairs that provide suitable selectivity, resolution, and materials corresponding to the USP definitions for filler types "L17/19/34" and "L58," this product is ideal for isocratic separation using water or diluted acids as solutes. This simplifies HPLC system requirements and eliminates the need to use hazardous organic solvents. [Features] ■ Resolution and materials corresponding to the USP definitions for filler types L17/19/34 and L58 ■ Separation of carbohydrates and alcohols through ligand exchange ■ Separation of organic acids through ion exchange *For more details, please refer to the related links or feel free to contact us.

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カラム『AdvanceBio オリゴヌクレオチド』

独自のテクノロジーを使用!高pH移動相に対する耐性を大幅に高めている

『AdvanceBio オリゴヌクレオチド』は、高効率、2.7μmの表面多孔質 Poroshell粒子が特長のバイオ医薬品分析用LCカラムです。 エンドキャップ処理を施したC18相で オリゴヌクレオチドの選択性を高めます。 さらに、分離能標準を用いて当製品をバッチテストすることで、 一貫した性能を実現しています。 【特長】 ■HPLCおよびUHPLCシステムの長寿命カラムによる、  オリゴヌクレオチドの高分解能分離 ■結果の信頼性の向上 ・Poroshellの効率的な粒子形態により高分解能分離を実現 ■コストの削減 ・高pHに対して優れた耐性を持つ堅牢な化学修飾シリカによりカラムを長寿命化 ※詳しくはPDF資料をご覧いただくか、お気軽にお問い合わせ下さい。

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Agilent BioHPLC Column

If all columns and consumables are used correctly, the lab work will proceed smoothly!

The "Agilent BioHPLC Column" is designed to perform high-precision and high-speed characterization analysis of monoclonal antibodies and other intact proteins, aggregates by SEC, charge variants by IEX, intact mass by reversed phase, primary structure, post-translational modifications (PTM), and cleaved glycan analysis by hydrophilic interaction liquid chromatography. To further enhance the productivity of chromatography labs, smooth operations can be achieved if all columns and consumables are used correctly. 【Features】 ■ Achieves fast and consistent biopharmaceutical analysis ■ Designed to provide consistent performance ■ Underlying technology of the column improves accuracy and productivity ■ Supports speeding up analysis and enhancing lab productivity *For more details, please refer to the PDF materials or feel free to contact us.

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Reverse Phase ODS Column

ODS columns that can be used with 100% aqueous mobile phase. There are examples of analyses such as water-soluble vitamins.

The "Ascentis Express 90 Å AQ-C18 U/HPLC" is a reverse phase ODS column that can be used for basic, acidic, and neutral polar compounds under 100% aqueous conditions. There are examples of analysis including comparisons of separation behavior with conventional C18, analysis of pyrethroid insecticides, organic acid analysis, and analysis of water-soluble vitamins. [Analysis Examples] ■ Comparison of separation behavior with conventional C18 ■ Pyrethroid insecticides ■ Organic acid analysis ■ Water-soluble vitamins *For more details, please refer to the PDF document or feel free to contact us.

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"Poroshell 300" LC column for biopharmaceutical analysis

In addition to protein separation for analysis, it can also be used for LC/MS separation with ammonia-containing mobile phases!

The "Poroshell 300" is an ideal LC column for the analysis of biopharmaceuticals, designed for the rapid separation of proteins and peptides. This column, featuring unique particles, maintains sharp and efficient peak shapes even at high flow rates. With a unique particle structure that combines a porous silica surface thin phase and a hard silica core, it shortens the diffusion distance of proteins, enabling practical high-speed HPLC separations for peptides and proteins up to 500–1,000 kDa. 【Features】 ■ The Poroshell 300 column, bonded with StableBond phases (C18, C8, C3), exhibits excellent stability and sensitivity in TFA and formic acid mobile phases. ■ Usable at pH 2 to 11, it achieves unique separations and high selectivity. *For more details, please refer to the PDF document or feel free to contact us.

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CHEMCO MASK-ENV Column

High-performance pre-treatment column to prevent clogging of the column by humic acid.

The CHEMCO MASK-ENV column is a high-selectivity column developed for the pretreatment of environmental water samples in column switching methods. By introducing hydrophilic functional groups (sulfonic acid polymer) near the surface of a hydrophobic polymer filler through the MASK modification method, efficient concentration of target analytes and cleanup of interfering components can be achieved.

  • Water quality testing
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Liquid chromatography column "PEEK empty column"

Accumulated know-how and the diverse needs of our customers are being fulfilled!

Basa Manufacturing Co., Ltd. is committed to providing better products based on many years of experience and achievements, keeping in mind the diverse needs of our customers. We offer not only analytical columns but also preparative columns, packing fillers, and various connecting parts (adapters). We can produce custom orders according to application and dimensions, so we look forward to hearing from you. 【Features】 ○ Inert PEEK is used in the liquid contact parts ○ No adsorption/modification of the target components or rust formation ○ Pressure tests with water have confirmed retention at 40 MPa ○ Filling tests at 30 MPa have also been confirmed For more details, please contact us or download the catalog.

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Long-chain and cis-trans isomer analysis column

There are examples of analysis for vitamin K1 isomers! It is suitable for reverse phase separation of geometric isomers and positional isomers.

The "Ascentis Express 160 Å C30 U/HPLC" is a column for the analysis of long-chain and cis-trans isomers. It is suitable for the reversed-phase separation of geometric isomers and positional isomers. There are examples of analysis including a comparison of separation behavior with C18, four types of tocopherols, five types of fat-soluble vitamins, and vitamin K1 isomers. [Analysis Examples] ■Comparison of separation behavior with C18 ■Four types of tocopherols ■Five types of fat-soluble vitamins ■Vitamin K1 isomers *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Eclipse XDB-CN

Introducing the ZORBAX normal phase HPLC columns with both combined and non-combined filler series!

We would like to introduce the 'ZORBAX Eclipse XDB-CN' that we handle. We offer both bonded and non-bonded filler series, which are suitable for the normal phase analysis of basic compounds. It equilibrates faster than ZORBAX Rx-Sil and can be used for many normal phase analyses similar to Rx-Sil. 【Features】 ■ Manufactured from high-purity Rx-SIL ■ Equilibrates faster than ZORBAX Rx-Sil and can be used for many normal phase analyses similar to Rx-Sil *For more details, please refer to the PDF document or feel free to contact us.

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Biomonolith <Protein A, Protein G>

Used primarily for the quantification of IgG (calculation of fermentation potency)!

We would like to introduce our bio-pharmaceutical analysis LC column, 'Bio Monolith <Protein A, Protein G>'. It enables high-speed and accurate quantification of mAb antibody titers and allows for the purification of small amounts of mAb for further characterization. It can be used with HPLC and UHPLC systems, including Agilent 1100, 1200, and 1260 Bio Inert Quaternary LC. The Bio Monolith Protein G column is suitable for the purification of IgG molecules that do not bind to Bio Monolith Protein A. 【Features】 ■ Separation analysis of all IgG subclasses (human and mouse) ■ Protein G provides different selectivity for IgG molecules that do not bind to Protein A ■ Separation unaffected by flow rate ■ High-speed movement between mobile and stationary phases ■ Fixation of method parameters ■ Significant reduction in analysis time and buffer usage *For more details, please refer to the related links or feel free to contact us.

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HAS/IgG Multiple Affinity Removal Column

Removal of albumin and IgG! This improves the sensitivity of subsequent sample LC/MS analysis and electrophoresis.

We would like to introduce the "HAS/IgG Multiple Affinity Removal Column" that we handle. The column format is "4.6 x 50mm," and custom-made column sizes and spin cartridges are also available. By using the multiple affinity removal system, approximately 69% of total proteins (albumin and IgG) can be removed. With an optimized two-buffer system, the column can be reused with very high reproducibility for over 200 injections, and it is provided with a convenient column reagent starter kit. 【Features】 ■ The serum injection volume per analysis is 100μL for a column with an inner diameter of 4.6mm. ■ The protocol and column are optimized for the analysis of human biological samples. ■ Compatible with most FPLC or HPLC pumps and equipment, including Agilent 1200 instruments. ■ Human serum albumin/IgG affinity removal spin cartridges are also available. ■ Up to 20,000μL of plasma/serum can be processed until the column's lifespan is reached (for a column with an inner diameter of 4.6mm). *For more details, please refer to the related links or feel free to contact us.

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JP18 Pharmaceutical Test Item LC Analysis Relevant Column

Introduction of each pharmaceutical item! In addition to the specified columns, test items and others are also included.

On our website, we introduce HPLC columns that comply with the 18th edition of the Japanese Pharmacopoeia (Notification No. 220 by the Ministry of Health, Labour and Welfare, dated June 7, 2021). We have listed pharmaceuticals such as "Actinomycin D," "Aclarubicin," "Acrinol Hydrate," and "Azathioprine" by each item. Additionally, if there are no catalog products for the corresponding structure of fillers or column sizes, we indicate "No applicable products." There is a possibility that we can propose custom columns, so please contact us if you have any requests. [Items Listed (Partial)] ■ Actinomycin D ■ Aclarubicin ■ Acrinol Hydrate ■ Azathioprine ■ Acyclovir *For more details, please refer to the related links or feel free to contact us.

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BioResolve RP column for protein separation

New column for reverse phase separation of intact proteins and their subunits.

The analysis of proteins with high molecular weight and complex structures is accompanied by many difficulties. Waters has developed a new reversed-phase column based on the feedback and requests from researchers and analysts who are actually working on protein analysis. The BioResolve RP mAb Polyphenyl column uses solid core particles with a particle size of 2.7 μm and incorporates novel functional groups, achieving not only improved separation but also significant improvements in carryover compared to low molecular weight compounds. At the same time, it reduces issues commonly associated with high temperatures and ion-pair reagents used in reversed-phase separation of proteins. Additionally, we offer guard columns and standards specifically designed for protein analysis, along with comprehensive support such as user-friendly procedures and analysis examples for those conducting protein analysis for the first time.

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FlexFire Metal-Free Column

Introduction to LC/MS is also easy! There are analyses that can be made possible with hardware changes!

The "FlexFire Metal-Free Column" is an HPLC/UHPLC column that has a significant effect on compounds such as phosphate groups and metal coordination. Traditionally, these compounds have been analyzed using stainless steel columns with phosphate buffer methods. By using this product, it is possible to develop methods using formate buffers, making it easier to introduce into LC/MS. 【Features】 - Prevents adsorption by avoiding contact with metals - Allows for direct elution of compounds - Has a significant effect on compounds such as phosphate groups and metal coordination - Enables method development using formate buffers *For more details, please refer to the PDF document or feel free to contact us.

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Liquid chromatography column "stainless steel empty column"

Accumulated know-how and diverse customer needs are being fulfilled!

Hakusho Co., Ltd. is committed to providing better products based on many years of experience and achievements, keeping in mind the diverse needs of our customers. We offer not only analytical columns but also preparative columns, packing devices, and various connecting parts (adapters). We accept custom orders based on application and dimensions, so we look forward to hearing from you. 【Features】 - Capable of supporting a wide range of HPLC applications - A rich variety of inner diameters, high inner surface precision, and low diffusion column ends - Proposing high-precision products based on years of experience and achievements - Custom empty columns can be manufactured to meet customer needs - Available in screw type and ferrule type (swagelok type) For more details, please contact us or download the catalog.

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DNAPac PA200/PA200 RS Column

High separation of oligonucleotide chains and stereoisomers! Anion exchange column.

The "DNAPac PA200 HPLC/DNAPac PA200 RS (High-Speed Separation) UHPLC Column" is a column designed for the high separation of ssDNA and RNA. The selectivity for oligonucleotides can be controlled by various parameters such as the pH of the mobile phase, solvents, and the use of different salts, allowing for adjustments in separation to meet the specific requirements of applications. We provide structural analysis and quantification methods utilizing high-resolution mass spectrometry and anion exchange columns for UHPLC. 【Features】 ■ High separation of oligonucleotide chains and stereoisomers ■ Improved separation and throughput ■ Control of selectivity and retention through the pH of the mobile phase, type of salt, and type of mobile phase ■ Separation of n from n-1 bases *For more details, please refer to the PDF document or feel free to contact us.

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Column "AdvanceBio MS Spent Media"

Rapid MS-based workflow! No need for sample derivatization, saving time and resources.

The "AdvanceBio MS Spent Media" is a HILIC column that can separate non-derivatized amino acids and other polar metabolites in cell culture media with high speed, excellent sensitivity, and reproducibility, making it suitable for mass spectrometry. By combining it with Agilent InfinityLab LC series instruments and Agilent MS instruments, a comprehensive solution for cell culture component analysis is achieved. The AdvanceBio MS Spent Media analysis is an innovative solution designed for the generation and characterization of biomolecules, as a new product in the Agilent AdvanceBio family. 【Features】 ■ Achieves excellent peak shape and recovery rates even for challenging ionic metabolites ■ Baseline chromatographic separation capability for isomers of leucine and isoleucine ■ Columns tested with amino acids to ensure quality and performance ■ Excellent analytical sensitivity with a mobile phase suitable for MS ■ Compatible with both HPLC and UHPLC systems *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Bonus-RP

Introducing a new technology that enables three-dimensional protection! ZORBAX reverse phase HPLC columns.

The "ZORBAX Bonus-RP" has a unique structure that incorporates polar amide groups into a long alkyl chain. This new bonding method suppresses the interaction between basic compounds and the silica substrate, significantly improving peak shape even for particularly difficult-to-separate basic compounds. It also demonstrates excellent stability at pH 2, making it suitable for improving the peak shape of basic compounds with significant tailing in many other alkyl-type stationary phases. 【Features】 ■ Excellent peak shape for challenging basic compounds at low and neutral pH ■ Unique selectivity among reverse phase columns ■ New technology that embeds polar groups and allows for steric protection ■ Usable with 100% aqueous mobile phases *For more details, please refer to the PDF document or feel free to contact us.

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Technical Data: Chiral Column for Reverse Phase Ultron ES-OVM

Effective for metabolic studies in blood! A column that is valuable in the fields of pharmaceuticals, pesticides, food, and organic synthesis.

The "Ultron ES-OVM" is an optical separation HPLC column that has many excellent features, such as being easy to use in reverse phase mode, having good peak shape, and being suitable for the study of trace metabolites. It uses amino-modified silica gel with proteins that are resistant to denaturation as the stationary phase, allowing for the analysis of plasma samples without any pre-treatment. This document includes the basic properties of the ligand, examples of separation degree adjustment, and case studies of compound analysis using the Ultron ES-OVM. We encourage you to read it. [Contents] ■ Basic properties of the ligand ■ Mechanism of separation ■ Selection of mobile phase ■ Relationship between pH and capacity factor ■ Relationship between salt concentration and capacity factor *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Extend-C18

Improvement of retention, separation ability, and peak shape in base compounds! Introducing ZORBAX reverse phase HPLC columns.

The "ZORBAX Extend-C18" utilizes a groundbreaking bidentate C18-C18 bonding technology, enabling the development of high-separation methods in high pH regions while being a silica-based column. The unique bidentate bonding and double-end capping enhance stability at high pH. It improves retention, separation capability, and peak shape for basic compounds. To extend column life, it is preferable to use triethylamine, ammonium hydroxide, or pyrrolidine as buffer solutions, while phosphate buffers are not recommended. 【Features】 - High separation efficiency and long lifespan in high pH regions (up to pH 11.5) - Improved stability at high pH due to unique bidentate bonding and double-end capping - Higher efficiency and superior peak shape compared to polymer-based columns - High sensitivity and separation for LC/MS analysis of peptides and proteins *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut Atrazine

Bond Elut Atrazine is designed for the extraction of dealkylated metabolites, specifically desethylatrazine and deisopropylatrazine.

Bond Elut Atrazine is designed specifically for the extraction of atrazine from aqueous matrices, as well as its dealkylated metabolites, desethylatrazine and deisopropylatrazine. The 120μm particle size and suitable tube diameter allow for fast flow rates, making it ideal for processing large volumes of water samples. Additionally, the bed mass capacity of Bond Elut Atrazine is optimized to ensure the reliable recovery of atrazine and its polar metabolites, demonstrating sufficient capacity. By using an analytical method that combines Bond Elut Atrazine with Varian's HPLC diode array detector system, quantitative analysis of atrazine and its dealkylated metabolites in river water is possible even at extremely low concentrations (0.02 ppb). This concentration level is significantly lower than the EPA's standard (3 ppb) and the values set by European agencies (0.1 ppb and 0.5 ppb). The rapid and highly reproducible extraction and analysis methods are reliable and can minimize the consumption of organic solvents. *For more details, please refer to the PDF materials or feel free to contact us.*

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Multiple Affinity Removal Column Mouse3

Remove three types of excess protein with a simple method.

The "Agilent Mouse3 Multiple Affinity Removal Column" is designed to remove three types of proteins, including the abundant albumin, from mouse plasma samples, making it easier to separate and identify low-concentration proteins in mouse serum. By removing high-concentration proteins, the dynamic range of analysis can be effectively expanded, enhancing the performance of LC/MS and electrophoresis analyses of serum samples. (This product is not intended for use other than research purposes.) *For more details, please refer to the related links or feel free to contact us.

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Ultra-low pressure particle size 3μm column Sumipax ODS-D-210SLP

Ultra-low pressure HPLC column achieving the highest standard of low pressure with a particle size of 3 μm.

This is a general-purpose column made from high-purity silica gel manufactured based on the technology our company has cultivated over many years. The SUMIPAX ODS series offers a wide range of high-performance columns, each with different characteristics. We believe that by selecting the optimal column from a variety of options with differing specificities, we can meet all analytical needs.

  • Analytical Equipment and Devices
  • column

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