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culture Product List

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Culture of iPS cells using CellPet 3D-iPS.

Culture of iPS cells using CellPet 3D-iPS.

The obtained spheroids are uniform! Introducing the culture of iPS cells [Free technical materials available].

We would like to introduce the "CellPet 3D-iPS" three-dimensional rotating floating culture device for the culture of iPS cells. With the CELLFLOAT system, we were able to obtain spheroids that maintained their undifferentiated state. The size of the spheroids had a mode of 220-240 μm, with 61.9% (1865 out of 3013) existing within the range of 200-300 μm. You can download technical materials from the related catalog below. Please take a look. 【Overview】 ■ Cell type: 253G1 (Pre-culture: 253G1 cells cultured in Φ60 dishes coated with iMatrix511 (Nippi)) ■ Culture medium: AK02N (Ajinomoto) ■ Detachment solution: EDTA (5 mM) ■ Culture vessel: Disposable culture vessel <10ml> ■ Culture conditions (rotation speed): 40 rpm *For more details, please refer to the PDF materials or feel free to contact us.

  • Other Culture
  • Cell dispersion reagent
  • Other cell research
  • culture

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Optimization of Feed Strategy for Enhanced Fed-Batch Processes

Optimization of Feed Strategy for Enhanced Fed-Batch Processes

The enhanced fed-batch process allows biopharmaceutical manufacturers to improve operational efficiency and accelerate mAb production!

This document discusses the optimization of feed strategies for enhanced fed-batch processes using "Cellvento ModiFeed Prime Feed." It introduces a study examining the effects of starting the feed from the first day of culture, rather than on the third day as in traditional fed-batch processes, to achieve successful cultivation at high cell densities. Additionally, various feed schedules have been evaluated to optimize cultivation performance. [Contents (partial)] ■ Future prospects of enhanced fed-batch ■ Impact of first-day feed on medium formulation ■ Importance of first-day feed in enhanced batch cultivation ■ Evaluation of feed schedules ■ Control of cultivation environment *For more details, please download the PDF or feel free to contact us.

  • Other Culture
  • culture

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