The obtained spheroids are uniform! Introducing the culture of iPS cells [Free technical materials available].
We would like to introduce the "CellPet 3D-iPS" three-dimensional rotating floating culture device for the culture of iPS cells. With the CELLFLOAT system, we were able to obtain spheroids that maintained their undifferentiated state. The size of the spheroids had a mode of 220-240 μm, with 61.9% (1865 out of 3013) existing within the range of 200-300 μm. You can download technical materials from the related catalog below. Please take a look. 【Overview】 ■ Cell type: 253G1 (Pre-culture: 253G1 cells cultured in Φ60 dishes coated with iMatrix511 (Nippi)) ■ Culture medium: AK02N (Ajinomoto) ■ Detachment solution: EDTA (5 mM) ■ Culture vessel: Disposable culture vessel <10ml> ■ Culture conditions (rotation speed): 40 rpm *For more details, please refer to the PDF materials or feel free to contact us.
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