ザルトリウス・ジャパン List of Products and Services

We would like to introduce an interview regarding our products. This time, we are featuring Dr. Kohei Takeshita from the SPring-8 Center for Synchrotron Radiation Research at RIKEN. He started using our Octet Biolayer Interferometry System in 2019. We spoke with him about how Octet is utilized in protein science and structural biology. 【Excerpt from the content】 - A user-friendly device that is maintenance-free and intuitive to operate - Significantly reduces preparation and waiting times for efficient assay condition exploration - Naturally transitions from synthetic chemistry to life sciences, paving the way for researchers *For more details, please refer to the PDF document or feel free to contact us.

The Octet N1 system is a personal assay system that enables label-free, real-time measurement and analysis of biomolecular interactions using Bio-Layer Interferometry (BLI) and Dip and Read assays. It significantly reduces initial setup costs and allows for simple and rapid kinetics analysis (Ka, Kd, KD), protein detection and quantification, and assay development. 【Features】 ■ Measurement possible with just 4μL of a small sample ■ Ability to measure crude samples such as cell lysates in their unpurified state ■ Label-free assay that minimizes time and cost associated with labeling ■ No complicated setup, shutdown procedures, or maintenance required ■ Compact size that can be installed in a space smaller than A4 size ■ Kinetics analysis possible with reduced initial setup costs *For more details, please refer to the PDF document or feel free to contact us.

Octet Systems is an automated assay system that enables label-free, real-time measurement and analysis of interactions between biomolecules using Bio-Layer Interferometry (BLI) and Dip and Read assays. It eliminates the bottlenecks of traditional SPR methods, achieving high throughput, compatibility with diverse samples, and ease of use. The Octet system offers five models, allowing for the selection of the optimal system based on the number of samples, applications, and budget. 【Features】 ■ High-throughput assay method facilitates multi-sample assays and optimization of experimental conditions. ■ Capable of measuring crude samples such as cell lysates in their unrefined state. ■ Allows measurement of low-solubility samples using buffers containing high concentrations of DMSO solutions. ■ No complicated setup, shutdown procedures, or maintenance required. ■ The Dip and Read assay enables the recovery and reuse of samples after measurement. *For more details, please refer to the related catalog or feel free to contact us.

To minimize the manufacturing costs of biopharmaceuticals while maintaining high standards of quality and safety, technologies are being pursued to streamline the drug development workflow and reduce time. Analytical techniques that enhance efficiency while maintaining quality are essential in the development of biopharmaceuticals, from upstream processes such as cell line development to downstream processes including the determination of dynamic binding capacity of purification columns, impurity confirmation tests, and potency assays. The Octet system for analyzing biomolecular interactions meets the required demands with high sensitivity, high precision, and rapid measurement. ■ Screening of samples during cell line development ■ Monitoring through titer measurement in bioreactors ■ Determination of dynamic binding capacity of chromatography columns and characterization of purified products ■ Rapid and highly accurate detection of impurities such as host cell proteins and residual Protein A *For more details, please refer to the PDF document or feel free to contact us.

As a regulatory requirement, biopharmaceuticals must be QC tested using methods that are robust and appropriately qualified and validated under GMP conditions. The Octet system for analyzing biomolecular interactions is a reliable system that facilitates method development for in-process testing and lot release testing, and it can be easily transferred to QC. Due to its ease of use and high throughput characteristics, it can achieve up to 40 times the productivity of ELISA and up to 16 times that of SPR equipment in specific GMP applications. ■ The Octet CFR software and FB server functionality maintain secure and traceable electronic records. ■ The IQ/OQ/PQ package guarantees the required conditions and operations. ■ Validation support services for software and biosensors are provided. *For more details, please refer to the PDF materials or feel free to contact us.

In January 2020, the genomic sequence of COVID-19 was released, leading to many papers that brought breakthroughs related to the biology, vaccines, and therapeutic development of coronaviruses, featuring data from the Octet system for analyzing biomolecular interactions. The document "Advancing development of coronavirus vaccine and therapeutics," which can be downloaded from the catalog below, presents research papers that elucidate the binding kinetics and affinity, neutralization, cross-blocking, and potency of COVID-19 virus particles using the Octet system. ■ Rapidly screen and select optimal vaccine and therapeutic candidates ■ Ensure research progresses with the best candidates determined based on data *For more details, please refer to the PDF document or feel free to contact us. 【Contact Information】 Sartorius Japan K.K. BioAnalytics Tel: 03-6478-5200 E-mail: ForteBioJP@Sartorius.com

From industry-standard multi-cycle kinetics to patented OneStep(R), Onestep(R) Two Comp, OneStep(R) Pulse, and NeXtStep(TM) Gradient Injections, we support all research applications and assays. With OneStep(R) Injection Technology, there is no need to prepare multiple dilution series; users only need to prepare a single concentration of analyte solution, enabling measurements across a comprehensive concentration range for kinetics and affinity analysis. NeXtStep(TM) Injection Technology targets competitive assays, allowing users to determine the complete kinetics and affinity of the analyte in the presence of multiple competing substances from a single analyte concentration.

The "Octet SF3" is a device that uses surface plasmon resonance (SPR) technology. It suppresses baseline noise and drift, allowing for high-capacity injection. It enables high-throughput analysis of label-free biomolecular interactions. Octet has become a brand that offers two main label-free technologies: BLI and SPR. 【Main Features of Octet SF3】 "OneStep Gradient Injection" - No need to prepare a dilution series of the analyte concentration, saving time, reagents, and sample plate space. - A single concentration of the analyte diffuses according to the flow of the buffer, generating a concentration gradient of three digits or more. "NeXtStep Gradient Injection" - The behavior of the analyte in the presence of competing molecules can be quantified in a single injection. - Since competing substances are not included in the running buffer, multiple combinations of analytes and competing substances can be evaluated in a single assay. *For more details, please download the PDF or contact us.

The performance and detection capability have evolved significantly, providing excellent performance and detection ability. It excels in performance and reliability, supporting high sensitivity required in labs, long-duration unmanned automatic measurements, and highly flexible throughput. It flexibly accommodates accurate characterization of diverse analytes from discovery to manufacturing. We provide solutions for labs seeking a step-up in innovation and efficiency. - The performance of label-free analysis is outstanding, allowing for smooth decision-making. - The accuracy and efficiency of throughput have improved. - It is equipped with performance that can accommodate future research.