Unchained Labs (アンチェインドラブズ） List of Products and Services

In the development of vectors such as LNP (lipid nanoparticles) and AAV (adeno-associated virus), buffer exchange is an important step and presents various challenges. LNP requires rapid exchange, while AAV necessitates improvements in recovery rates and concentration, so a single solution is not always optimal. Unagi is a buffer exchange and concentration device developed to accommodate biomolecules for biological formulations and gene therapies, utilizing pressure-based ultrafiltration/diafiltration (UF/DF) technology. It achieves precise buffer exchange and concentration without reducing sample recovery rates. In this webinar, you will see the final exchange values, concentration values, and analytical characteristics under various conditions for LNP and AAV. ■ Date and Time: October 30 (Monday) 14:00~ ■ Speaker: Yuji Senoo, Field Application Scientist, Unchained Labs Inc. ■ Register here: https://unchainedlabs.zoom.us/webinar/register/5816780852848/WN_WfFOAUgtQGCq0tamdhQjWg

ADC (Antibody-Drug Conjugate) is a biopharmaceutical that is attracting attention due to its pharmacological activity and the excellent specificity of antibodies. However, the characterization of ADCs is very challenging due to the rarity of samples and their tendency to aggregate. In this webinar, we will explain how to obtain important information such as the concentration, labeling, stability, and viscosity of mAbs and their conjugates from just a few microliters of sample, along with actual data to demonstrate the evaluation of ADC characteristics. ■ Date and Time: June 16 (Friday) 14:00~ ■ Speaker: Yukinori Ooshiro, Field Application Scientist, Unchained Labs, Inc. ■ Register here: https://unchainedlabs.zoom.us/webinar/register/5816780852848/WN__LZqwPKFTvy2KTYnajByqg

The development of biopharmaceuticals is becoming mainstream, but compared to small molecule drugs, many challenges remain due to their complex structures and properties. For example, long-term stability tests when researching protein stability in biopharmaceuticals are generally conducted in the later stages of development. However, this approach carries the risk that issues with candidate drugs may only become apparent in the latter half of development. Additionally, controlling the viscosity of formulations is one of the important development requirements, yet obtaining viscosity data can take too long, which is also a challenge. In this webinar, we will tackle these challenges in biopharmaceutical development and introduce methods for conducting isothermal stability tests early in the development process, as well as a way to simultaneously measure the viscosity of multiple samples in just a few minutes without the need for cleaning. ■ Date and Time: May 22 (Monday) 14:00~ ■ Speaker: Yukinori Ooshiro, Field Application Scientist, Unchained Labs Inc. ■ Register here: https://unchainedlabs.zoom.us/webinar/register/5816780852848/WN_lsAhytF6R-ayPZLDougO3w

Lipid nanoparticles (LNPs) are currently one of the most notable delivery systems used in mRNA vaccines, gene therapy, cancer treatment, and more. However, their development requires a complex process involving formulation screening, control of size and quality, removal of organic solvents, and characterization, which demands significant effort and time. In this webinar, we will introduce methods to enhance the production of perfectly sized LNPs, evaluate their size and quality characteristics, perform buffer exchange and sample concentration, and analyze stability at each step of the development process workflow. ■ Date and Time: March 27 (Monday) 14:00~ ■ Speaker: Yukinori Ooshiro, Field Application Scientist at Unchained Labs ■ Register here: https://unchainedlabs.zoom.us/webinar/register/5816780852848/WN_ycxoqBXnScOgpzBx1dQ16g

Subject: In recent years, viruses have been widely used as modalities for gene therapy and vaccines, and research and development have been advancing. Adenoviruses are one of the representative viruses, but traditional evaluation methods are diverse and require a lot of time and effort. In this seminar, we will explain how Stunner can quickly and accurately resolve issues such as capsid titer, Full/Empty ratio, and the presence or absence of aggregation as a one-stop solution. We will also look at the application of adenovirus vector sample QC and the evaluation of various types of adenoviruses. ■ Date and Time: April 25 (Tuesday) 14:00~ ■ Speaker: Yukinori Ooshiro, Field Application Scientist, Unchained Labs, Inc. ■ Registration link: https://unchainedlabs.zoom.us/webinar/register/5816780852848/WN_OJ0mgL39TZiB_kUC3sojmw

AAV production, infectivity, Full/Empty ratio, and optimization of introduced genes require a lot of time. Additionally, during the formulation screening stage, many conditions need to be examined, such as different pH levels, salt concentrations, or additives. Our Unagi enables rapid and quantitative buffer exchange and concentration by pressurizing the sample solution on a dialysis membrane, allowing for the examination of numerous formulation conditions for AAV samples at once. Furthermore, Stunner can accurately assess AAV titer, Full/Empty ratio, and aggregation evaluation by simultaneously measuring UV/Vis and DLS in a high-throughput manner. In this webinar, we will explain how to accurately and quickly conduct formulation considerations and physical property evaluations of AAV samples using our Stunner and Unagi. ■ Date and Time: February 21 (Tuesday) 14:00~ (approximately 40 minutes) ■ Speaker: Yukinori Ooshiro, Field Application Scientist, Unchained Labs, Inc. ■ Register here: http://bit.ly/3WU3IM9

Viscosity data is essential for the formulation development, characterization, and quality control of biopharmaceuticals. In recent years, biopharmaceuticals have been increasingly concentrated, leading to rising viscosity as a challenge, and its importance is growing. However, most current viscosity measurement devices require large amounts of samples and extensive working hours, making it difficult to obtain sufficient viscosity data. In this seminar, we will review actual measurement results of antibodies and other proteins using the high-speed micro-volume viscometer, Honeybun, designed for biological formulations, and delve into its excellent precision, accuracy, and throughput from low-volume samples. ■ Date and Time: September 5 (Monday) 14:00~ (approximately 30 minutes) ■ Speaker: Yukinori Ooshiro, Field Application Scientist, Unchained Labs, Inc. ■ Register here: https://bit.ly/3AOGnUf

We would like to introduce data using our stability evaluation tool, Stunner, in the currently trending AAV field. Speaker: Mr. Nelis Denys, Product Manager at Unchained Labs Here, you can watch webinars that have been held in the past. Please register using the link below (registration is one-time only, and you will have unlimited access to all videos, including this one). https://go.unchainedlabs.com/JPVideosForm

We would like to introduce data using our stability assessment tool, Stunner, in the field of LNP, which is currently attracting attention. Speaker: Mr. Nelis Denys, Product Manager at Unchained Labs Here, you can watch webinars that we have held in the past. Please register using the link below (registration is one-time only, and you will have unlimited access to all videos, including this one). https://go.unchainedlabs.com/JPVideosForm

Researchers of lipid nanoparticles (LNPs) spend a tremendous amount of time on screening, optimization, and scaling up experiments to achieve optimal performance, spot-on targeting, and controlled release of active pharmaceutical ingredients (APIs). Factors such as solubility, pH stability, payload release, and encapsulation all depend on particle size, shape, and structure. In other words, to obtain the right formulation, it is necessary to explore a vast number of formulation parameters and process parameters one by one, and even if a perfect formulation is found, scaling up usually means switching equipment and re-optimizing settings. In this webinar, we will introduce a platform, Sunny Suite, that can reduce the time spent on these LNP formulations. ■ Date and Time: February 26 (Monday) 14:00~ ■ Speaker: Yuji Senoo, Field Application Scientist, Unchained Labs, Inc. ■ Register here: https://unchainedlabs.zoom.us/webinar/register/3217065146675/WN_U1o3xQoZRAKHN-A-7b1yqg

The concentration of lipid nanoparticles is an important parameter that should be known for all batches of particles, but existing methods either cannot handle throughput, require dilutions that affect accuracy, or take a lot of time. Additionally, keeping the particles at the appropriate size is a goal for everyone, but it is not possible to detect all aggregates in the sample, and just collecting size data for all samples is quite challenging. In this webinar, you will learn how our newly updated Stunner can not only determine the size of LNPs and quantify particle concentration but also quantify payloads, providing unique information from various nanoparticle applications. *This webinar content was translated into Japanese and presented in Japanese by Nelis Denys, M.Sc., Market Manager at Unchained Labs, Inc. ■ Public viewing period: Until March 27, 2024 (Wednesday) ■ For details and viewing, click here >> https://go.unchainedlabs.com/l/907652/2024-03-04/xzbwj