KOTAIバイオテクノロジーズ List of Products and Services

"Bioinformatics Data Analysis" utilizes algorithms from information science and statistics to analyze Fastq data provided by customers. Comparisons are made between different cell populations, such as target group vs. comparison group. Additionally, sequencing can be performed from libraries created upon order, and information analysis can also be conducted. We are open to meeting other customer requests. Depending on the volume and content, we may propose a collaborative research format instead of a contracted analysis. [Contracted Analysis Contents] ■ Single-cell RNA-Seq Analysis ■ Single-cell Repertoire Analysis ■ CITE-Seq Analysis ■ Bulk Repertoire Analysis *For more details, please refer to the PDF document or feel free to contact us.

• Analysis and sequencing services
• Other analysis and sequence
• Other Analysis

"Single-cell repertoire analysis" allows for the examination of TCR/BCR expression at the single-cell level by assigning different barcodes to the mRNA of individual T cells/B cells. In standard repertoire analysis, TCR/BCR expression is comprehensively analyzed across all cells, but this analysis can detect repertoire expression with high sensitivity for each cell, and it is also capable of detecting combinations of TCR/BCR dimer sequences. Additionally, single-cell 5' RNA-Seq can also be performed. [Features] ■ Detection of rare TCR/BCR (Ig) expression ■ Identification of TCR/BCR (Ig) for each cell ■ Pair analysis of BCR (Ig) heavy/light chains or TCR alpha/beta chain sequences is possible ■ Single-cell 5' RNA-seq can be performed alongside TCR/BCR analysis *For more details, please refer to the PDF document or feel free to contact us.

• Analysis and sequencing services
• Other analysis and sequence
• Other Analysis

"Visium spatial gene expression analysis" allows for gene expression analysis while maintaining the positional information of cells from tissue sections. Cells from a tissue placed within a 6.5mm x 6.5mm area can be analyzed for 5000 spots. An average of 1 to 10 cells can be captured per spot, and mRNA analysis can be conducted while maintaining positional information by assigning a unique barcode to each spot. [Features] ■ Ability to analyze 5000 spots of cells from tissue within a 6.5mm x 6.5mm area ■ RNA expression analysis while preserving the positional information of cells within the tissue ■ Comparison of HE staining images with gene expression maps *For more details, please refer to the PDF materials or feel free to contact us.

• Gene expression analysis contract

KOTAI Biotechnologies Co., Ltd. is holding an opening campaign to commemorate the start of its contract services until June 30, 2020. Using the Chromium Single Cell Kit from 10x Genomics, we will perform library preparation, sequencing, and Cell Ranger analysis. Please take advantage of this campaign period. 【Campaign Details】 ■ Period: Until June 30, 2020 (Tuesday) for quoted estimates ■ Eligible Services - Single Cell Repertoire (BCR/TCR)-Seq - Single Cell RNA-Seq ■ Cell Acceptance Conditions - Frozen state - At least 1 million cells - Survival rate of 80% or higher - (For human biological samples and clinical specimens) Information must be anonymized *For more details, please refer to the PDF document or feel free to contact us.

• Cell-related contract
• Analysis and sequencing services
• Other contract services

Our company conducts patient personalization through immune response analysis. We extract cells related to the disease of interest from peripheral or tissue T/B cells. We perform analyses that are closer to disease phenotypes through single nucleotide polymorphism (SNP) analysis. We also identify combinations with existing biomarkers. [Analysis Examples] ■ Immune-related adverse effects of immune checkpoint inhibitors - Identification of immune responses specific to adverse effects by comparison with healthy individuals. *For more details, please download the PDF or contact us.

• Other Testing Contract
• Analysis and sequencing services
• Other analysis and sequence