ニッポンジーン List of Products and Services

This product is phenol used for RNA extraction by the AGPC method, pre-saturated with citrate buffer (pH 4.2). In the AGPC method, RNA is partitioned into the aqueous phase by using acidic phenol, allowing for the separation of RNA from DNA. The lower phase is Acid Phenol, and the upper phase is citrate buffer, with the lower phase being used. 【Features】 - Phenol used for RNA extraction by the AGPC method - Composition: 75% phenol, 0.1% 8-quinolinol, saturated with citrate buffer - Phenol is saturated with citrate buffer (pH 4.2) *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a solution mixed with TE saturated phenol, chloroform, and isoamyl alcohol in a ratio of 25:24:1. It is used for nucleic acid extraction from biological tissues and for protein removal after enzymatic reactions of nucleic acids. Isoamyl alcohol helps reduce the foam generated during use. The TE saturated phenol (66% phenol) used has been pre-saturated with TE and contains 8-hydroxyquinoline. 【Features】 ■ TE saturated phenol (66% phenol) contains 8-hydroxyquinoline ■ Composition: 50(37)% TE saturated phenol, 48% chloroform, 2% isoamyl alcohol ■ It has two layers, with the upper layer being the TE layer for oxidation prevention, and the lower layer consisting of phenol/chloroform/isoamyl alcohol. *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

"TE Saturated Phenol" is used for extracting nucleic acids from biological tissues and for protein removal after nucleic acid enzymatic reactions. It is pre-saturated (equilibrated) with TE buffer [10 mmol/l Tris-HCl (pH 8.0), 1 mmol/l EDTA]. Additionally, this product has a two-layer structure, with the yellow lower layer being TE saturated phenol and the colorless upper layer serving as a TE layer for oxidation prevention. [Features] - Contains 0.1% (w/v) 8-hydroxyquinoline - Composition: 66% phenol, 0.1% 8-quinolinol, saturated with TE - pH: TE saturated phenol 7.2–8.2 *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

The product "20 x SSC" consists of 0.3M sodium citrate and 3M sodium chloride. We offer two types of packaging: a 500ml glass bottle and a 5L polyethylene container. Nippon Gene has a wide range of ready-to-use nuclease-free buffer products available. 【Product Details (Partial)】 <20 x SSC (500ml)> ■ Volume: 500ml x 1 ■ Storage: Room temperature ■ Container: Medium bottle *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a stock solution at 10 times the concentration of phosphate-buffered saline (PBS). It is a phosphate-buffered saline composed of sodium chloride, disodium hydrogen phosphate, potassium chloride, and dipotassium hydrogen phosphate, adjusted to have a pH of 7.4 for the working solution (1 x PBS) when used. It is very convenient as it does not take up space during storage and can be used simply by diluting it 10 times with water. 【Features】 ■ Does not take up space during storage and can be used simply by diluting it 10 times with water ■ Adjusted to have a pH of 7.4 for the working solution (1 x PBS) when used ■ Autoclaved, DNase-free, RNase-free *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a stock solution of 10 times concentrated tris-buffered saline (TBS). It is a buffer solution consisting of Tris, sodium chloride, and potassium chloride, adjusted to pH 7.4 with hydrochloric acid. It has a different composition from "20 x TBS (pH 7.4)." It is very convenient as it does not take up space during storage and can be used simply by diluting it 10 times with water. 【Features】 - Does not take up space during storage and can be used simply by diluting it 10 times with water - Buffer solution consisting of Tris, sodium chloride, and potassium chloride - Composition: 1,370 mmol/l NaCl, 26.8 mmol/l KCl, 250 mmol/l Tris - Autoclaved, DNase-free, RNase-free *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a stock solution at a 20 times concentration of tris-buffered saline. It is a buffer composed of tris(2-amino-2-hydroxymethyl)propane-1,2-diol and sodium chloride, adjusted to pH 7.4 with hydrochloric acid, making it suitable for reagent preparation due to its simple composition. Nippon Gene offers a wide range of ready-to-use, nuclease-free buffer products. 【Features】 ■ Does not take up space during storage; can be used by simply diluting 20 times with water ■ Adjusted to pH 7.4 with hydrochloric acid ■ Composition: 3 mol/l NaCl, 0.4 mol/l Tris-HCl (pH 7.4) ■ Autoclaved, DNase-free, RNase-free *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a 10% Sodium Dodecyl Sulfate (SDS) solution. It is available for a wide range of applications, including nucleic acid extraction and preparation of reagents for electrophoresis. SDS is a representative anionic surfactant and is an essential reagent for genetic engineering experiments, such as protein denaturation. 【Product Details】 ■ Volume: 100ml×1 / 500ml×1 ■ Storage: Room temperature ■ Container: Plastic bottle *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

The product is a 1 mol/l magnesium chloride aqueous solution. It can be used for a wide range of applications, including buffer preparation, as a DNase-free and RNase-free reagent. Nippon Gene offers a wide variety of ready-to-use nuclease-free buffer products. 【Product Details】 ■ Volume: 100ml x 1 ■ Storage: Room temperature ■ Container: Medium bottle *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a 10 mol/L ammonium acetate solution. It can be used for a wide range of applications, including ethanol precipitation of nucleic acids, as a DNase-free and RNase-free reagent. Nippon Gene offers a wide variety of ready-to-use nuclease-free buffer products. 【Product Details】 ■ Volume: 100ml x 1 ■ Storage: Room temperature ■ Container: Medium bottle *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a 3 mol/l sodium acetate solution adjusted to pH 5.2. It can be used for a wide range of applications as a DNase-free and RNase-free reagent, including ethanol precipitation of nucleic acids. Nippon Gene offers a wide variety of ready-to-use nuclease-free buffer products. 【Product Details】 ■ Volume: 100ml x 1 ■ Storage: Room temperature ■ Container: Medium bottle *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a 0.5 mol/l solution of ethylenediaminetetraacetic acid (EDTA). It is available for a wide range of applications, such as chelating agents and reagent preparation. EDTA is a chelating agent that forms chelate compounds with divalent metal ions such as calcium and magnesium. Many enzymes that require divalent metal ions (such as DNase I) are present, making it an essential reagent for genetic engineering experiments, including the inhibition of enzyme reactions. 【Features】 ■ Composition: 0.5M disodium ethylenediaminetetraacetate, adjusted to pH 8.0 with sodium hydroxide ■ Autoclaved ■ DNase-free ■ RNase-free *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

This product is a 1 mol/l Tris hydrochloride buffer that has been adjusted to various pH levels. It can be used for a wide range of applications, including buffer preparation, as a DNase-free and RNase-free reagent. Nippon Gene offers a wide selection of ready-to-use nuclease-free buffer products. 【Product Lineup】 ■1M Tris-HCl (pH 7.0) ■1M Tris-HCl (pH 7.5) ■1M Tris-HCl (pH 8.0) ■1M Tris-HCl (pH 8.5) ■1M Tris-HCl (pH 8.8) ■1M Tris-HCl (pH 9.0) *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

"TE (pH 8.0)" is a mixture of Tris hydroxymethyl aminomethane solution (pH 8.0) and ethylenediaminetetraacetic acid solution (pH 8.0) that has been autoclaved. It is a 1×TE buffer, ready for immediate use. It can be used for dissolving and storing DNA and RNA samples. Nippon Gene offers a wide range of ready-to-use nuclease-free buffer products. 【Features】 ■Composition: 10 mmol/l Tris-HCl (pH 8.0), 1 mmol/l EDTA-2Na (pH 8.0) ■Autoclaved ■DNase-free ■RNase-free *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

"DEPC treated Water" is water that has been processed using distillation, ion exchange, and filtration methods, to which diethyl pyrocarbonate (DEPC) has been added and then autoclaved. When conducting experiments involving RNA, DEPC treatment may be performed to ensure that reagents and instruments are RNase-free. To minimize the time required for preparing such reagents, we provide water that has been DEPC treated and autoclaved. Nippon Gene offers a wide range of immediately usable nuclease-free buffer products. [Features] - Water that has been treated with distillation, ion exchange, and filtration methods, to which diethyl pyrocarbonate has been added and autoclaved. - RNase-free and DNase-free water that has not undergone DEPC treatment is also available. *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

The product is water that has been treated by distillation, ion exchange, and filtration, and then autoclaved. It is suitable for a wide range of applications, including the dissolution of DNA and RNA samples, and is DNase-free and RNase-free. Nippon Gene offers a wide variety of ready-to-use nuclease-free buffer products. 【Product Details (Partial)】 <Distilled Water, Deionized, Sterile (100ml)> ■ Volume: 100ml x 1 ■ Storage: Room temperature ■ Container: Plastic bottle *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

The product "TE (pH 8.0), Nuclease free" is a solution that is commonly used for the dissolution and storage of nucleic acids, dispensed in 1mL aliquots of TE buffer (pH 8.0). It is a 1× TE buffer, ready to use immediately. Products are also available in 100mL and 500mL packaging. Additionally, it is autoclaved and nuclease-free, ensuring safe usage. 【Features】 ■ Autoclaved, DNase/RNase free ■ Aliquoted in a clean room ■ Safe for use in nucleic acid dissolution and storage ■ Convenient 1mL packaging for easy use ■ Manufactured in Japan *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

"Water, Nuclease free" is a product that contains water dispensed in 1mL portions, commonly used for dissolving and storing nucleic acids, as well as in PCR experiments. It comes in pre-packaged small portions that make it easy to use, reducing the risk of contamination from DNase, RNase, and other sources during use. It is autoclaved and nuclease-free, allowing for safe usage. 【Features】 ■ Autoclaved, DNase/RNase free ■ Dispensed in a clean room ■ Safe for use in PCR and for dissolving/storing nucleic acids ■ Convenient 1mL packaging ■ Manufactured in Japan *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

At Nippon Gene, we utilize the manufacturing know-how we have cultivated over more than 35 years to produce prepared solutions according to your specified composition, standards, and capacity. Furthermore, we can manufacture under desired conditions (e.g., animal-free, clean room) and accommodate quality inspections in accordance with sterility tests, pharmacopoeia, etc. 【Features】 ■ Supply products that have passed necessary quality tests and meet standards ■ Support from small scale to large scale ■ Accommodate sterility tests ■ Capable of manufacturing prepared solutions and OEM supply for various applications *For more details, please refer to the PDF document or feel free to contact us.

• Other Contract Life Science Specialization

Our company offers various contract manufacturing services such as "preparation solution manufacturing," "recombinant protein expression and purification using E. coli," and "kit manufacturing," allowing us to respond to our customers' needs with great attention to detail. We can accommodate requests for quality and process optimization, as well as small-scale production. <Examples of Contract Manufacturing> ■ Bulk supply services for enzymes and reagents ■ Custom solution preparation services ■ Custom kit manufacturing services ■ Recombinant protein purification services *For more details, please download the PDF or feel free to contact us.

• Other bioproduct raw materials

Our company develops and manufactures reagents for genetic engineering research. Leveraging over 38 years of accumulated manufacturing know-how, we respond meticulously to customer requests. Furthermore, we can accommodate optimization of manufacturing processes and quality, as well as small-scale production. 【Features】 ■ Small-scale production available ■ Supply of products that have passed quality testing *For more details, please download the PDF or contact us.

• Other contract services