バイオ未来工房 List of Products and Services

Many books on cell culture have been published so far, but there are very few that specialize in human mesenchymal stem cells (hMSC), and particularly, no books have been published on technical content. As a result, we received requests from stakeholders in research institutions for a text specifically focused on hMSC, leading to the release of the "Mesenchymal Stem Cell Handbook." This book is a practical guide that explains the methods of operation, focusing on the isolation and culture of mesenchymal stem cells from tissues, expansion culture, differentiation induction, and other applications. **You can download a PDF to view some of the content.** <Recommended for the following people!> - Those who are handling hMSC for the first time and do not know where to start - Those who want to know specific and efficient culture methods - Those who want a reference book to train culture technicians - Those who want to deepen their knowledge of hMSC It is available for purchase on Amazon, our company website, and the website of the Information Organization.

• Other cell research
• Other bio-related products
• Other Culture

We will conduct tests and experiments related to human mesenchymal stem cells. Since our establishment, our company has been engaged in research on human mesenchymal stem cells. Utilizing that experience, we will undertake experiments concerning human mesenchymal stem cells. If you are unsure about what kind of experiments to conduct or wish to evaluate using human stem cells, please feel free to consult with us. <Main Test Contents> - Gene expression analysis (such as real-time PCR) - Protein quantification (such as ELISA) - Cell proliferation tests - Cell surface marker tests (flow cytometry) etc...

• Flow cytometer

We measure nine human viruses (HIV1, HIV2, HCV, HBV, HTLV, ParvoB19, WNV, EBV, CMV) recommended for testing to ensure the quality and safety of human (same species) somatic stem cell processing pharmaceuticals using real-time PCR. For HIV, HIV1, HIV2, HCV, HBV, HTLV1, and HTLV2, measurements can be performed individually. Testing kit: Virus Test Kit (manufactured by Takara Bio) Please feel free to consult us regarding acceptable specimens, required specimen volumes, prices, etc.

• Other analysis and evaluation services

We will conduct a sterility test on samples such as cell supernatants. <Sterility Test (Microbial Rapid Method)> Using a method based on the gas measurement method from the Japanese Pharmacopoeia, we will culture for one week and detect changes in the amounts of carbon dioxide and oxygen as changes in fluorescence, allowing us to detect microorganisms such as aerobic bacteria, anaerobic bacteria, and fungi. Please feel free to consult us regarding acceptable samples, required sample volumes, prices, etc.

• Other analysis and evaluation services

We will conduct an endotoxin test on samples such as culture supernatants. <Endotoxin Test> The endotoxin testing method is based on the reaction in which endotoxins derived from Gram-negative bacteria activate the LAL (Limulus Amebocyte Lysate) extracted from horseshoe crab blood cells, causing gelation. Since it occurs through sequential enzymatic reactions, it is a colorimetric method that uses the wave erosion resulting from the hydrolysis of synthetic substrates by enzymes as an indicator. Please feel free to consult us regarding acceptable samples, required sample volumes, prices, etc.

• Other analysis and evaluation services

We will conduct a mycoplasma detection test for samples such as culture supernatants. Our company offers a choice between two methods: real-time PCR or MycoAlert. <MycoAlert Method> This measurement method utilizes the activity of enzymes from mycoplasmas, which are present in the majority of 180 species of mycoplasma and not found in eukaryotic cells. <Real-time PCR Method (TaKaRa Mycoplasma qPCR Detection Kit)> Using the real-time PCR method, we can detect seven species of mycoplasma genes specified by official methods such as the Japanese Pharmacopoeia in about one hour. Additionally, we use a validated method as indicated in the "Mycoplasma Detection Test C. Nucleic Acid Amplification Test (NAT)" of the 18th Revised Japanese Pharmacopoeia reference information for cell substrates used in the manufacture of biotechnology-derived pharmaceuticals/bio-origin pharmaceuticals. Please feel free to inquire about acceptable samples, required sample volumes, prices, etc.

• Other analysis and evaluation services

We will conduct differentiation induction tests for each cell from the human mesenchymal stem cells you possess, along with evaluations to confirm differentiation. The cells that can be induced to differentiate are as follows: - Adipocytes - Osteoblasts - Chondrocytes - Neurons After differentiation induction, confirmation of differentiation will be performed using the following staining methods: - Adipocytes → Oil Red O staining - Osteoblasts → Alizarin Red S staining - Chondrocytes → Alcian Blue staining - Neurons → Antibody staining If you have any other desired culture conditions or requests, please feel free to contact us. We can also accommodate differentiation induction only or staining only.

• Other Culture

<Improved MSC Separation Medium (Animal-Free)!> - The name has been changed to BSCM-SP1 Mesenchymal Stem Cell Separation Medium (Animal-Free, Albumin-Free). - It is now possible to isolate and culture from tissues such as adipose tissue, umbilical cord, dental pulp, and bone marrow. - Please continue to use serum at 1-3% during culture as before. - This is a research medium for regenerative medicine that shows excellent proliferation specifically for human mesenchymal stem cells. - The risk of viral infection from human and animal-derived materials and albumin-derived substances has been eliminated. *Please use in conjunction with the stem cell separation substrate. Feel free to contact us if you have any requests. 【Features】 - A highly safe medium that is both animal-free and albumin-free. - Specializes in the isolation and culture of stem cells from human adipose tissue, umbilical cord, dental pulp, and bone marrow. - Maintains differentiation potential (adipocytes, osteoblasts, chondrocytes, neurons). - Retains cell surface markers (CD73, 90, 105). - Can be stored in a refrigerator. - Manufactured domestically. *For more details, please refer to the PDF document or feel free to contact us.

• Cell dispersion reagent

The "Alcian Blue Staining Kit" is a kit designed for regenerative medicine research that allows staining for 48 well plates (96 samples). The eluted dye can be measured for absorbance (OD620nm). Additionally, it is specially formulated for eluting Alcian Blue stained human chondrocytes. 【Features】 ■ Capable of fixation, staining, and extraction of cells ■ Elution solution specialized for human chondrocytes ■ Allows measurement of absorbance after dye elution ■ Free from toxic substances ■ Made in Japan *For more details, please refer to the PDF document or feel free to contact us.

• Staining Reagents

The "Chondrocyte Differentiation Induction Kit" is a kit designed for regenerative medicine research that allows for the differentiation of human mesenchymal stem cells into chondrocytes when cultured in cell culture plates (48-well, flat-bottom recommended) for 3 to 4 weeks. It can induce differentiation equivalent to approximately two 48-well plates. Additionally, the chondrocyte differentiation induction medium contains components such as TGF-β and dexamethasone, and is formulated to efficiently promote the differentiation of human mesenchymal stem cells. 【Operating Instructions (Simplified Version)】 ■1. Seed human mesenchymal stem cells in a cell culture plate and culture overnight with seed medium (37°C, 5% CO2). ■2. Add chondrocyte differentiation induction medium and continue culturing. ■3. Change the medium every 3 to 5 days and culture for 3 to 4 weeks. ■4. To confirm differentiation into chondrocytes, perform staining and check gene expression, etc. *For more details, please refer to the PDF document or feel free to contact us.

• Cell dispersion reagent

The "Alizarin Red S Staining Kit" is a kit designed for regenerative medicine research that allows staining for 48 well plates (96 samples). By eluting the dye from cells stained with Alizarin Red S, it is possible to measure absorbance. Additionally, it is prepared with staining concentrations and pH suitable for calcified human osteoblasts. Please feel free to contact us if you have any requests. 【Features】 - Capable of fixation, staining, and extraction of cells - Staining solution specialized for human osteoblasts - Allows measurement of absorbance by eluting the dye - No toxic substances used - Made in Japan *For more details, please refer to the PDF document or feel free to contact us.

• Staining Reagents

The "Osteoblast Differentiation Induction Kit" is a kit designed for regenerative medicine research that allows for the differentiation of human mesenchymal stem cells into osteoblasts when cultured in cell culture plates (48-well, flat bottom) for 1 to 2 weeks. It enables differentiation induction equivalent to approximately 2 plates of 48 wells. Additionally, the osteoblast differentiation induction medium contains components such as β-glycerophosphate and dexamethasone, and is formulated to efficiently promote the differentiation of human mesenchymal stem cells. 【Operating Instructions (Simplified Version)】 ■1. Seed human mesenchymal stem cells in a cell culture plate and culture overnight in a seeding medium (37°C, 5% CO2). ■2. Remove the medium and add the osteoblast differentiation induction medium for culture. ■3. Change the medium every 2 to 3 days and culture for 1 to 2 weeks. ■4. To confirm differentiation into osteoblasts, perform staining and check gene expression, etc. *For more details, please refer to the PDF document or feel free to contact us.

• Cell dispersion reagent

The "Oil Red O Staining Kit" is a kit designed for regenerative medicine research that allows staining for 96 samples (two 48-well plates). By eluting the pigment from fat droplets stained with Oil Red O, it is possible to measure absorbance. Additionally, the staining concentration and pH are optimized for staining fat droplets. Please feel free to contact us if you have any requests. 【Features】 ■ Capable of cell fixation, staining, and extraction ■ Staining solution specialized for human adipocytes ■ Absorbance can be measured by eluting the pigment ■ No toxic substances used ■ Made in Japan *For more details, please refer to the PDF document or feel free to contact us.

• Staining Reagents

The "Adipocyte Differentiation Induction Kit" is a kit designed for regenerative medicine research that allows human mesenchymal stem cells to differentiate into adipocytes when cultured in cell culture plates (48-well, flat bottom) for 1 to 2 weeks. It enables differentiation induction equivalent to about 2 plates of 48 wells. Additionally, the adipocyte differentiation induction medium contains components such as insulin and dexamethasone, and is formulated to efficiently promote the differentiation of human mesenchymal stem cells. 【Features】 ■ By diluting the adipocyte differentiation induction medium with a dilution medium, it is possible to examine suitable differentiation induction conditions for each cell. ■ Can be used with cell culture plates other than 48-well plates. *For more details, please refer to the PDF document or feel free to contact us.

• Cell dispersion reagent

The "Fibroblast Isolation Kit" is designed for regenerative medicine research and minimizes the risk of viral infection from human or animal sources by using albumin-free (animal-free) culture media. By simply placing a cover glass on the dermis for culture, it is possible to increase fibroblast numbers. Additionally, fibroblasts can be isolated without the use of digestive enzymes, which minimizes damage to the cells. 【Features】 ■ Easy to operate ■ No enzymes used ■ High safety (albumin-free) ■ Isolation of high-purity stem cells *For more details, please refer to the PDF document or feel free to contact us.

• Cell dispersion reagent

<MSC Proliferation Medium (Animal-Free) Improved!> - Renamed to BSCM-PL1 Mesenchymal Stem Cell Proliferation Medium (Animal-Free, Albumin-Free). - Some formulations have been changed, improving the proliferation capability compared to the previous version. - Please continue to add serum at 2-4% during culture as before. - This is a research medium for regenerative medicine that shows excellent proliferation specifically for human mesenchymal stem cells. - The risk of viral infection from human and animal-derived materials and albumin has been eliminated. Please use by adding 2-5% FBS or human serum. Feel free to contact us if you have any requests. 【Features】 - Serum-free, xenofree, animal-free, albumin-free → A medium focused on safety, allowing for autologous culture using self-serum → Small performance differences between lots - Excellent proliferation stability specifically for human mesenchymal stem cells → Improved proliferation stability due to formulation changes - Refrigerated transport and storage → Easy to handle - Maintenance of differentiation potential (adipocytes, osteoblasts, chondrocytes, neurons) - Retention of cell surface markers (CD73, 90, 105) - No coating required for culture vessels - Made in Japan

• Cell dispersion reagent

The 『BMK-R003』 is a substrate for separating adipose stem cells that allows for the easy and rapid isolation of high-purity stem cells from a small amount of human adipose tissue. *As part of a campaign until June 30, 2025, new purchasers can buy the stem cell separation substrate at half the regular price! *Please note that this campaign is only available for purchases made through our company website. *For more details, please check our company website. The non-woven fabric with a PE-PP core-sheath structure is coated with hydroxyapatite, and by culturing human adipose tissue, bone marrow, or umbilical cord tissue on this substrate, it is possible to isolate human mesenchymal stem cells (hMSC) that produce a rich extracellular matrix. 【Features】 ■ Enables separation of stem cells with minimal damage to the cells ■ No enzymatic treatment is required, allowing for quick preparation for culture (no collagenase used) ■ Utilizes the characteristics of stem cells to achieve high-purity separation ■ Stem cells adhered to the substrate can be detached using trypsin treatment ■ Separation is possible with a small amount of tissue ■ Sterilized and ready for immediate use ■ Retains differentiation potential and cell surface markers *Please feel free to contact us.

• Cell dispersion reagent

The "BMK-R001,004" is a kit that allows for the easy separation of adipose stem cells from human adipose tissue without the use of collagenase. *As part of a campaign until March 31, 2025, new purchasers can buy the adipose stem cell separation kit at half the regular price! *Please note that this campaign is only available for purchases made through our company website. *For more details, please check our company website. The kit includes two types of media: "BMK-R001," which contains FBS (1%), and "BMK-R004," an albumin-free (animal-free) medium that requires you to add your own serum. If you have any requests, please feel free to contact us. [Features] - Easy to operate - No collagenase used - Can separate from small amounts of tissue - High purity stem cell separation - Cells adhered to the substrate can be detached with trypsin treatment - Medium specialized for the separation and proliferation of adipose stem cells - Maintenance of differentiation potential *For more details, please download the PDF or feel free to contact us.

• Cell dispersion reagent