ブルカージャパン List of Products and Services

The combination of trapped ion mobility spectrometry (TIMS) and quadrupole time-of-flight (QTOF) technology enables 4D-Proteomics with the 'timsTOF Ultra'. It features a new Captive Spray Ionization (CSI) Ultra ion source optimized for larger capillaries and vortex gas flow, a new fourth-generation TIMS (trapped ion mobility separation) XR cell, and a 14-bit digitizer. 【Features】 ■ Expands the scope of single-cell research ■ Complements genomic approaches with unprecedented sensitivity ■ Immunopeptidomics analysis from just a few thousand cells ■ Maximizes sensitivity by combining timsTOF Ultra with Pepsep Ultra ■ PaSER has enhanced data visualization and QC run support in addition to the traditional Run&Done with the transition to ProteoScape.

• Analytical Equipment and Devices

The "timsTOF fleX MALDI-2" is a mass spectrometer (TIMS-QTOF, MALDI) that realizes subcellular imaging functionality through the pairing of the "microGRID" and "MALDI-2 technology" incorporated in the "timsTOF fleX." By using PASEF for LC-MS/MS identification and matching spatial localization, it is possible to confirm multi-level multi-omics expression in label-free tissues and identify their locations. Additionally, it is composed of an established sample preparation protocol, with hardware and software fully integrated to cover the analysis workflow. 【Features】 ■ Enables analysis of chemical groups that are prone to ion suppression ■ No need for physical hardware replacement ■ Simply switch between MALDI and MALDI-2 with one click in the software ■ Easy calibration of the device ■ Measurements can be initiated immediately using methods tested by scientists

• Analytical Equipment and Devices

The 'timsTOF Pro 2' equipped with new PASEF technology is a mass spectrometer (TIMS-QTOF) that paves the way for 4D multi-omics applications. It achieves three times the ion capacity, maximizing coverage of peptide and protein sequences. With proven robustness, high sensitivity, and fast MS/MS acquisition, it becomes an ideal platform for 4D proteomics, 4D metabolomics, and 4D lipidomics. 【Features】 ■ Analysis compatible with dual TIMS and CCS ■ PASEF delves deeper into complex samples ■ Maximizes coverage of peptide and protein sequences ■ dia-PASEF enhances identification reliability ■ High-sensitivity phosphoproteomics and isomer separation

• Analytical Equipment and Devices

The "timsTOF HT" is a mass spectrometer (TIMS-QTOF) equipped with the fourth generation TIMS-XR and advanced digitizer technology. It achieves a wide dynamic range and analytical depth in quantitative proteomics of cells and tissues. Its unique device design enables robust performance and routine operation for thousands of samples without the need for cleaning. Additionally, ion mobility separation has been added to the quadrupole time-of-flight mass spectrometer. 【Features】 ■ Expands the possibilities of high-throughput 4D-Proteomics ■ Achieves a sequencing speed of 150 Hz ■ Improves analytical depth with the high charge capacity of the fourth generation TIMS-XR ■ TIMS-based ion mobility separation and CCS-compatible analysis ■ New features of PaSER maximize identification depth and throughput

• Analytical Equipment and Devices

The "timsTOF SCP" for quantitative single-cell biology research conducts unbiased deep single-cell 4D proteomics, immunopeptidomics, piproteomics, and PTM analysis, complementing scRNA-seq. It is equipped with the "PASEF" solution, suitable for shotgun proteomics. The introduction of dual orthogonal reflection to the trapped ion mobility spectrometry (TIMS-QTOF) has enabled routine operations of thousands of samples without the need for cleaning the instrument. 【Features】 ■ Expands the scope of single-cell research ■ Changes the shape of the ion source and adopts a 1mm capillary ■ Dramatically improves proteomics performance ■ Analysis compatible with dual TIMS and CCS ■ Suitable for workflows in immunopeptidomics and other enrichments

• Analytical Equipment and Devices

Surface plasmon resonance (SPR) is an optical-based label-free detection technology for real-time monitoring of interactions between two or more molecules. The throughput, flexibility, and sensitivity of the SPR platform allow researchers to characterize interactions between biomolecules in various binding studies. It can be used to study a wide range of molecules, from ions, fragments, and small molecules to proteins and viruses. SPR provides biophysical data such as affinity, kinetics, and thermodynamics. The combination of three elements—the detector, sensor surface, and sample delivery system—determines the performance of the experiment.

• Analytical Equipment and Devices

**Features** - Rapid analysis at 50 pixels/second with clear images - Improved pixel reproducibility with 10kHz smartbeamTM 3D - Spatial resolution with pixel size <5μm enables more advanced information exploration - Durability significantly enhanced by a newly designed ion source - Wide dynamic range achieved with a 10-bit digitizer - MS/MS mass range up to 8 kDa - 3-stage gridless design: uncompromising maximum resolution across the entire molecular weight range

• Analytical Equipment and Devices

The Burker's patented long-life smartbeam laser operating at up to 200 Hz enables rapid sample analysis. Maintenance tasks performed with button operation are quick and do not require breaking the vacuum, maximizing uptime. No tools are needed. The combination of quick replacement of target plates and low-downtime ion source cleaning using a dedicated IR laser allows for faster analysis. Everything is aimed at enhancing laboratory productivity. 【Features】 ■ Rapid and easy quality control ■ Wide mass range ■ Automatic polymer analysis ■ Long-life smartbeam laser operating at up to 200 Hz

• Analytical Equipment and Devices

In routine work, technologies required at the research level are also used. The Bruker compact QTOF has the following features: - Provides high-precision MS and MS/MS data with sensitivity in the order of a few picograms. - Achieves reliable trace analysis from complex, high-background matrices by expanding the dynamic range. - Ensures mass accuracy below 1 ppm through true isotope pattern automatic calibration for clear spectra. - Maintains high resolution even in ultra-fast chromatography with 50 GBit/second sampling technology. The compact combines the robustness of Bruker's orthogonal time-of-flight mass spectrometer with 10-bit digitizer technology, enabling the detection and identification of compounds with an unmatched dynamic range.

• Analytical Equipment and Devices