BioJapan 2019

Comprehensive quantification and identification of trace proteins is possible!

Exosomes are gaining attention as targets with new biomarkers, and there is a demand for high-precision analysis of the proteins contained in exosomes. When analyzing blood samples, it is necessary to identify extremely small amounts of target molecules that are only present in exosomes secreted from the disease site, among the overwhelming majority of exosomes derived from normal cells. For this purpose, proteomics analysis methods that allow for the identification of more proteins and quantitative comparisons between samples are the optimal means. In DIA proteome analysis, we conduct proteome analysis using the Data-independent acquisition (DIA) method, which is renowned for its analytical depth and quantitativeness, with the state-of-the-art mass spectrometer Q-Exactive HF-X (Thermo Fisher Scientific). This method allows for the observation of up to 8,000 types of proteins in a single analysis and enables quantitative comparisons of individual proteins between samples. This service is a one-stop service that consistently handles everything from sample preparation to DIA proteome analysis, so customers can obtain high-depth proteome analysis data simply by sending in their samples.

• Contract Analysis
• Analysis Services
• Other contract services

We will accurately produce antibodies using screening methods such as exosomes!

We offer a step-by-step "antibody production contract service" tailored to your needs. We select antibodies that recognize the three-dimensional structure of target proteins present on the membrane surface using exosomes expressing the target protein, among other methods.

• Other contract services

You can directly quantify exosomes from blood samples and cell culture supernatants!

These are three types of products for the relative quantification of exosomes containing CD9, CD63, or CD81 molecules. - CD9/CD9 Exosome ELISA Kit - CD63/CD63 Exosome ELISA Kit - CD81/CD81 Exosome ELISA Kit Features: - Exosomes contained in blood samples or cell culture supernatants (serum-free) can be directly quantified. - No special equipment is required; measurements can be made with a standard plate reader. - Instead of using exosomes themselves, which lack storage stability as standard reagents, stability and reproducibility are ensured by using standard beads with a particle size of 200 nm that are coated with CD9 or CD63. - Relative quantification of each sample is possible by correcting with CD9, CD63, or CD81 standard beads that mimic exosome structures. - Exosomes are captured using solid-phase CD9, CD63, or CD81 antibodies, and detected with HRP-labeled CD9, CD63, or CD81 antibodies.

• others

We provide total support for exosome ELISA measurements and more.

We offer a contract service for the production of exosome detection tools that express and purify proteins in animal cells as an immunogen for antibodies against exosome surface markers. We provide comprehensive support for research on exosome detection methods, focusing on the production of recombinant proteins, the creation of monoclonal antibodies, the establishment of ELISA measurement systems, and exosome ELISA measurements. 【Service Details】 ■ Production of recombinant proteins ■ Creation of monoclonal antibodies against exosome surface markers ■ Establishment of ELISA measurement systems ■ Exosome ELISA measurements *For more details, please download the PDF or feel free to contact us.

• Other contract services

We would like to express our congratulations on the continued prosperity of your esteemed company. Our company will be exhibiting at the following trade show, where we will introduce a number of products, including new ones. We sincerely apologize for the inconvenience during your busy schedule, but we would like to invite you to attend this event. 【 Exhibition Name 】 31st Measurement and Measurement Exhibition INTERMEASURE 2024 Official Website: https://intermeasure.org/itms2024/ 【 Dates 】 From __ (Wednesday) to __ (Friday), 202_ 【 Venue 】 Tokyo Big Sight (Tokyo International Conference Center) East Hall (Booth Number: ___) 【 Exhibited Products 】 - Air Leak Tester - Hydrogen Leak Detector - Pin Hole (Reference Test Piece) and more. For more details, please refer to the "Related Materials" (PDF file) below.

Nitto Kohki will exhibit at the "H₂ & FC EXPO Spring International Hydrogen and Fuel Cell Exhibition" held at Tokyo Big Sight, West Exhibition Hall 2, from February 28 (Wednesday) to March 1 (Friday), 2024. [Booth Number: W14-63] The quick fluid coupling "Coupler" is a product that allows for the rapid connection and disconnection of piping for various fluids such as air, water, oil, and chemical solutions. At this exhibition, we will primarily showcase the high-pressure hydrogen filling quick fluid coupling "HHV Coupler."

☆☆☆ Urgent Visit to Japan & Emergency Event Announcement ☆☆☆ Dr. Yuval Levy, CEO of ISCFDC and developer of EPPIC, who has been involved in CFD research at NASA Ames Research Center for about five years, is coming to Japan! He will demonstrate and explain advanced CFD technologies, application cases, and the latest features of EPPIC himself. □ Date and Time □ July 31, 2026 (Friday) 13:00-18:00 (Registration starts at 12:30) <Part 1: Post-Processor Session> 13:00-16:40 <Part 2: Pre-Processor Session> 16:50-17:50 <Networking Event> 18:10-19:40 □ For more details □ Visit the Hulinks page https://www.hulinks.co.jp/seminar/eppic/

[Viewer Benefits Available] Explaining Tips for LC in a Short Seminar Do you have concerns such as "I'm unsure about column selection," "I'm struggling to optimize analysis conditions," or "I have challenges analyzing compounds with weak retention" in LC analysis? Waters will hold a seminar titled "Must-Listen! Advanced LC Application Seminar" where you can learn practical LC analysis know-how in just 30 minutes. In this seminar, we will cover useful topics in a total of six sessions, including tips for choosing detectors, how to utilize reverse phase columns, scaling up to preparative LC, approaches for analyzing hydrophilic and highly polar compounds, case studies of low-adsorption HPLC, and improving purity through solid-phase extraction. Each session is scheduled to start at 15:00. Event Themes: • October 6 (Tuesday) - Session 1: Tips for Choosing Detectors • October 13 (Tuesday) - Session 2: Tips for Choosing Columns • October 21 (Wednesday) - Session 3: Tips for Preparative LC • October 27 (Tuesday) - Session 4: Approaches for Analyzing Hydrophilic and Highly Polar Compounds • November 5 (Tuesday) - Session 5: Case Studies of Low-Adsorption HPLC • November 10 (Tuesday) - Session 6: Tips for Solid-Phase Extraction

In liquid biopsy research, we will introduce a technical evaluation of proteome analysis to obtain reliable data from trace plasma, as well as case studies on data quality verification using the Contamination Index and Simple Western.