[20th Annual Meeting of the Japanese Society for Regenerative Medicine Web Event] Co-hosted Academic Seminar 4 "Gene-Modified T Cell Therapy" Speaker: Dr. Hiroshi Tamaki (Professor, Mie University Graduate School)

Study on Quality Control through Parameter Measurement in iPS Cell Culture Supernatant

Focusing on "cell proliferation," one of the indicators of cell quality, we compared the results of cell proliferation and culture medium component analysis when changing the culture conditions. This is the result data from an experiment where the frequency of medium exchange was set to three conditions during the maintenance culture of iPS cells in a feeder-free environment, followed by three passages. In this verification, we used the cell culture environment analysis device "BioProfile FLEX2." The "BioProfile FLEX2" is a non-invasive, low-sample-volume device that can measure up to 16 items, including metabolites and cell viability, in just 4 minutes and 30 seconds, or 2 minutes with individual modules. 【Product Specifications】 Analysis Items: Glucose/Lactate/Glutamine/Glutamate/NH4+/Na+/K+ Ca++/pH/pO2/pCO2/Total Cell Density/Viable Cell Density/Viability/Cell Diameter/Osmolality Automatic Calibration: 2 hours (fixed)/Time required 2 minutes Operating Environment: Temperature 10–30°C, Humidity 20–85% 【Features】 ■ Simultaneous analysis of up to 16 items ■ Measurement time: as short as 2 minutes, as long as 4 minutes and 30 seconds (depending on measurement items) ■ Measurement of consumption and production rates of major metabolites ■ Compatible with multiple shapes of sample containers ■ Equipped with an auto-sampling function for continuous measurement

• Other Culture
• Other bio-related products
• Other analytical and testing equipment

Presentation of verification materials exploring the application to the evaluation of cell quality and experimental systems!

This document is a verification report that measures parameters in the culture supernatant during the maintenance culture of iPS cells and the differentiation induction process from iPS cells to dopamine-producing neural progenitor cells, exploring applications for evaluating cell quality and experimental systems. In addition to the experimental content and the composition of reagents and media, it discusses differentiation induction and maintenance culture. 【Contents】 ■ Purpose ■ Experimental Content ■ Reagents and Media Composition - Differentiation induction into neural progenitor cells - iPS cell culture ■ Discussion *For more details, please refer to the PDF document or feel free to contact us.

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An automatic analysis device for cell culture environments that achieves "shortened measurement time" and "significant reduction in sample volume"! All 16 items can be measured with just a small amount of sample.

The "BioProfile FLEX2" is an analytical device that utilizes micro-sensor technology. This technology allows the "BioProfile FLEX2" to achieve "reduced maintenance related to electrodes," "shortened measurement time," and "significantly reduced sample volume," making it an automatic analysis device for cell culture environments. It can measure all 16 parameters with a minimal sample volume, completing all 16 measurements in just 4 minutes and 30 seconds, or 2 minutes for individual modules. 【Features】 ■ Monitoring the progress of cell culture ■ Quantification of key components in the culture medium ■ Early detection of abnormalities through regular monitoring ■ Capability to measure multiple parameters with one device, consolidating the functions of multiple devices into one ■ Numerical backing for the timing of medium exchange *For more details, please request documentation or view the PDF data available for download.

• Other physicochemical equipment

Although I have heard that verification efficiency at the RTL stage improves with verification methodologies using SystemVerilog such as UVM, many designers may not fully understand what can be done with SystemVerilog and how to use it. In this seminar, we will introduce the basic functions and usefulness of SystemVerilog verification using a sample design. 1. Design Overview 2. UART Test Environment 3. Assertions (SVA & PSL) 4. Functional Coverage 5. Random Test Bench 6. DPI-C

The RTCA/DO-254 guidance recommends that applicants define and implement HDL coding standards. This work begins with a planning process that includes the definition of the standard, how it will be implemented, and, if the process is automated, the evaluation methods for tools or results. A key issue is that the guidance does not have clearly defined formal standards, leading to questions about how to conduct a detailed review of HDL code. In this webinar, we will demonstrate the use of ALINT-PRO to enforce coding standards and highlight the benefits of streamlining the overall design review to meet the objectives of DO-254.

[Speaker] Takahiro Tachikawa, President of International University and Professor at the Graduate School of International Management [Key Lecture Content] In February of this year, the 7th Energy Basic Plan was approved by the Cabinet. What is the essence of this plan that the media and others do not convey? How will this plan impact the development and social implementation of hydrogen, ammonia, and next-generation fuels (synthetic methane, synthetic liquid fuels, green LP gas, etc.)? This lecture will delve into these points. In particular, it will focus on the content of the risk scenario, which is viewed as the main scenario among stakeholders in the energy industry, and the newly defined role of natural gas as an "important energy source even after achieving carbon neutrality." [Lecture Items] 1. The 7th Energy Basic Plan 2. Hydrogen 3. Ammonia 4. Synthetic Fuels 5. Risk Scenario 6. Natural Gas Shift 7. Q&A / Business Card Exchange

[Lecturer] Professor Shiro Kawabata, Faculty of Science and Technology, Hosei University [Key Lecture Content] Prime Minister Ishiba has positioned 2025 as the inaugural year for the industrialization of quantum technology and has indicated a policy to strengthen support for the industrialization of quantum technology. Additionally, at the G7 Summit held in Canada in June 2025, a joint statement titled "Joint Statement on the Future of Quantum Technology," focusing on quantum technology centered around quantum computers, was adopted. Quantum computers are computers that utilize principles of quantum mechanics, such as superposition and quantum entanglement, for information processing. By using quantum computers, it becomes possible to solve several mathematical problems, such as factorization, machine learning, quantum chemistry calculations, finance, and linear simultaneous equations, exponentially faster compared to classical computers. As a result, major global corporations and startups are globally expanding their development and business in quantum computer hardware and software. This seminar will introduce the basics of quantum computers, the latest research and development trends, business trends, technical challenges, and future prospects.

[Speaker] Shintaro Kobayashi, Group Manager, ICT and Content Industry Consulting Department, Nomura Research Institute, Ltd. [Key Lecture Content] The Personal Information Protection Law is undergoing fundamental changes to promote AI development. The Personal Information Protection Commission has proposed a shift towards a risk-based system where companies voluntarily address risks. A key feature of this new system is the establishment of a framework that allows the use of personal information for AI development without the need for individual consent. In this lecture, based on the policy of legal reform, we will carefully explain the points for advancing data utilization by leveraging the legal benefits of the new system, as well as how to establish privacy governance that should drive these efforts, incorporating specific initiatives and examples of solutions. [Lecture Items] 1. Trends in the amendment of the Personal Information Protection Law 2. Practical methods for utilizing personal information without individual consent 3. PETs for safely utilizing AI 4. Achieving governance efficiency and sophistication through AI 5. Q&A / Business card exchange