[20th Annual Meeting of the Japanese Society for Regenerative Medicine Web Event] Co-hosted Academic Seminar 4 "Gene-Modified T Cell Therapy" Speaker: Dr. Hiroshi Tamaki (Professor, Mie University Graduate School)

Study on Quality Control through Parameter Measurement in iPS Cell Culture Supernatant

Focusing on "cell proliferation," one of the indicators of cell quality, we compared the results of cell proliferation and culture medium component analysis when changing the culture conditions. This is the result data from an experiment where the frequency of medium exchange was set to three conditions during the maintenance culture of iPS cells in a feeder-free environment, followed by three passages. In this verification, we used the cell culture environment analysis device "BioProfile FLEX2." The "BioProfile FLEX2" is a non-invasive, low-sample-volume device that can measure up to 16 items, including metabolites and cell viability, in just 4 minutes and 30 seconds, or 2 minutes with individual modules. 【Product Specifications】 Analysis Items: Glucose/Lactate/Glutamine/Glutamate/NH4+/Na+/K+ Ca++/pH/pO2/pCO2/Total Cell Density/Viable Cell Density/Viability/Cell Diameter/Osmolality Automatic Calibration: 2 hours (fixed)/Time required 2 minutes Operating Environment: Temperature 10–30°C, Humidity 20–85% 【Features】 ■ Simultaneous analysis of up to 16 items ■ Measurement time: as short as 2 minutes, as long as 4 minutes and 30 seconds (depending on measurement items) ■ Measurement of consumption and production rates of major metabolites ■ Compatible with multiple shapes of sample containers ■ Equipped with an auto-sampling function for continuous measurement

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Presentation of verification materials exploring the application to the evaluation of cell quality and experimental systems!

This document is a verification report that measures parameters in the culture supernatant during the maintenance culture of iPS cells and the differentiation induction process from iPS cells to dopamine-producing neural progenitor cells, exploring applications for evaluating cell quality and experimental systems. In addition to the experimental content and the composition of reagents and media, it discusses differentiation induction and maintenance culture. 【Contents】 ■ Purpose ■ Experimental Content ■ Reagents and Media Composition - Differentiation induction into neural progenitor cells - iPS cell culture ■ Discussion *For more details, please refer to the PDF document or feel free to contact us.

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An automatic analysis device for cell culture environments that achieves "shortened measurement time" and "significant reduction in sample volume"! All 16 items can be measured with just a small amount of sample.

The "BioProfile FLEX2" is an analytical device that utilizes micro-sensor technology. This technology allows the "BioProfile FLEX2" to achieve "reduced maintenance related to electrodes," "shortened measurement time," and "significantly reduced sample volume," making it an automatic analysis device for cell culture environments. It can measure all 16 parameters with a minimal sample volume, completing all 16 measurements in just 4 minutes and 30 seconds, or 2 minutes for individual modules. 【Features】 ■ Monitoring the progress of cell culture ■ Quantification of key components in the culture medium ■ Early detection of abnormalities through regular monitoring ■ Capability to measure multiple parameters with one device, consolidating the functions of multiple devices into one ■ Numerical backing for the timing of medium exchange *For more details, please request documentation or view the PDF data available for download.

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This is a course where you can systematically learn the knowledge and skills necessary for management positions from the perspectives of leadership and labor management, and strengthen your "ability to motivate people." It is open to one participant per company. Content: - The ideal image of a leader - Team building that creates results - Ways to communicate that engage people - Methods for guiding and developing subordinates - Rules of labor management - Prevention of harassment - Mental health in the workplace Here are some comments from participants: - I have dreamed of becoming the best team, so I will share this with my members and take steps forward! - If I communicate what I am not doing to the staff, I won't gain their support. I will express it through my own actions. - The phrase "Strategies to enhance motivation without human relationships are meaningless" resonated with me. - I had no knowledge about labor issues, and it was a learning experience. I will study to be able to respond as a leader. We look forward to your participation. *Details can be downloaded from "Product Information." *For inquiries and applications, please contact us below or visit our website or call us. *Registration will close once we reach capacity.

We want to improve our operations with AI, but what exactly can we do... For those who want to actually try out and compare services, we have an announcement. The electronic contract and document management service paperlogic will be exhibiting at the 'Business Innovation Fair 2025 Autumn in Makuhari' hosted by Otsuka Shokai on November 13th (Thursday) and 14th (Friday) at the Hotel Green Tower Makuhari. Seminars on business improvement and AI utilization will also be held, so if you are nearby, please come and visit us. ● We will also be conducting product demonstrations of paperlogic at the venue. The biggest feature of paperlogic is that it is easy to use and simple, yet it complies with all document management regulations, including the Electronic Bookkeeping Act. We invite you to experience this simplicity firsthand.

At our center, we regularly hold a lecture series called the "Environmental Science Seminar" aimed at providing and promoting information related to the environment and hygiene for citizens. This year, it will take place on Saturday, November 15th. We are pleased to have Dr. Takeshi Ochiai from the Kanagawa Prefectural Institute of Industrial Technology, a local independent administrative institution, deliver a lecture titled "The Future of Living Created by Photocatalysts." Photocatalysts are materials that use natural forces such as light and water to decompose and remove dirt and harmful substances. While it may sound complicated, they are actually utilized in everyday products like wall materials, window glass, and air purifiers. In this seminar, we will clearly introduce photocatalysts, a dream material that is environmentally friendly, safe, and contributes to the future of society. Additionally, we will set up an exhibition booth where you can experience the effects of photocatalysts, so please come and try it out.

Our company will exhibit equipment at the "69th Space Science and Technology Union Conference." We sincerely look forward to your visit.

Japan Filtration Group Co., Ltd. will be exhibiting at the "10th Drink Japan" held at Makuhari Messe. On December 5th (Friday), we plan to introduce our research facility "Filter Lab," established in Boston, equipped with advanced equipment and cultivation facilities, to respond timely to various customer needs for verification and product development in the exhibitor seminar area within the exhibition venue. The director, who has been involved in filter experiments for many years, will explain microbial control through the sterilization and verification methods of Levilactobacillus brevis and Alicyclobacillus acidoterrestris, which are spoilage bacteria found in fruit juice and wine.