[20th Annual Meeting of the Japanese Society for Regenerative Medicine Web Event] Co-hosted Academic Seminar 4 "Gene-Modified T Cell Therapy" Speaker: Dr. Hiroshi Tamaki (Professor, Mie University Graduate School)

Study on Quality Control through Parameter Measurement in iPS Cell Culture Supernatant

Focusing on "cell proliferation," one of the indicators of cell quality, we compared the results of cell proliferation and culture medium component analysis when changing the culture conditions. This is the result data from an experiment where the frequency of medium exchange was set to three conditions during the maintenance culture of iPS cells in a feeder-free environment, followed by three passages. In this verification, we used the cell culture environment analysis device "BioProfile FLEX2." The "BioProfile FLEX2" is a non-invasive, low-sample-volume device that can measure up to 16 items, including metabolites and cell viability, in just 4 minutes and 30 seconds, or 2 minutes with individual modules. 【Product Specifications】 Analysis Items: Glucose/Lactate/Glutamine/Glutamate/NH4+/Na+/K+ Ca++/pH/pO2/pCO2/Total Cell Density/Viable Cell Density/Viability/Cell Diameter/Osmolality Automatic Calibration: 2 hours (fixed)/Time required 2 minutes Operating Environment: Temperature 10–30°C, Humidity 20–85% 【Features】 ■ Simultaneous analysis of up to 16 items ■ Measurement time: as short as 2 minutes, as long as 4 minutes and 30 seconds (depending on measurement items) ■ Measurement of consumption and production rates of major metabolites ■ Compatible with multiple shapes of sample containers ■ Equipped with an auto-sampling function for continuous measurement

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• Other bio-related products
• Other analytical and testing equipment

Presentation of verification materials exploring the application to the evaluation of cell quality and experimental systems!

This document is a verification report that measures parameters in the culture supernatant during the maintenance culture of iPS cells and the differentiation induction process from iPS cells to dopamine-producing neural progenitor cells, exploring applications for evaluating cell quality and experimental systems. In addition to the experimental content and the composition of reagents and media, it discusses differentiation induction and maintenance culture. 【Contents】 ■ Purpose ■ Experimental Content ■ Reagents and Media Composition - Differentiation induction into neural progenitor cells - iPS cell culture ■ Discussion *For more details, please refer to the PDF document or feel free to contact us.

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An automatic analysis device for cell culture environments that achieves "shortened measurement time" and "significant reduction in sample volume"! All 16 items can be measured with just a small amount of sample.

The "BioProfile FLEX2" is an analytical device that utilizes micro-sensor technology. This technology allows the "BioProfile FLEX2" to achieve "reduced maintenance related to electrodes," "shortened measurement time," and "significantly reduced sample volume," making it an automatic analysis device for cell culture environments. It can measure all 16 parameters with a minimal sample volume, completing all 16 measurements in just 4 minutes and 30 seconds, or 2 minutes for individual modules. 【Features】 ■ Monitoring the progress of cell culture ■ Quantification of key components in the culture medium ■ Early detection of abnormalities through regular monitoring ■ Capability to measure multiple parameters with one device, consolidating the functions of multiple devices into one ■ Numerical backing for the timing of medium exchange *For more details, please request documentation or view the PDF data available for download.

• Other physicochemical equipment

We will be exhibiting at the J AGRI (J Agri) / Agricultural Materials EXPO held at Gran Messe Kumamoto from May 22 (Wednesday) to May 24 (Friday), 2024. 【Booth Number】: B1-43 Outdoor Exhibition Area Japan's largest comprehensive exhibition for agriculture and livestock, Agricultural WEEK, will be renamed J AGRI starting in 2024. This is a place where you can actually see, touch, and compare products. At our booth, we will showcase our lightweight resin flooring boards "JULIGHT / SUPER JULIGHT / RAKURAKUBAN" along with new products. Starting with the agricultural-specific flooring board "RAKURAKUBAN," which can be easily laid in various scenes such as around greenhouses, agricultural work sites, temporary parking lots, for the movement of agricultural machinery and light trucks, and for protecting truck beds, there are various ways to use the lightweight resin flooring boards "JULIGHT / SUPER JULIGHT series." We offer a variety of sizes and protrusion shapes tailored to customer needs, and they are lightweight enough to be carried by hand, with excellent impact resistance, as well as superior water resistance, weather resistance, and durability, providing outstanding features not found in plywood or steel plates. We sincerely look forward to your visit.

Dear Sir or Madam, We hope this message finds you well and that your company continues to prosper. FUKUDA will be exhibiting at the 28th INTERPHEX Week Tokyo. At the exhibition, we will be showcasing 100% inspection equipment compliant with Container Closure Integrity Testing (CCIT) standards, as well as sampling inspection machines. We sincerely invite you to visit our booth at this opportunity and would greatly appreciate your attendance despite your busy schedule. Yours sincerely, [Dates] May 20 (Wed) - 22 (Fri), 2026 10:00-17:00 (JST) [Venue] Makuhari Messe, Japan 8 Hall Booth No. 41 - 36

Dear Sir or Madam, We hope this message finds you well and that your company continues to prosper. FUKUDA will be exhibiting at the AUTOMOTIVE ENGINEERING EXPOSITION 2026 YOKOHAMA. At this exhibition, we will be showcasing testing equipment such as Air Leak Tester for EV and Equipment Mounted Type Hydrogen Leak Detector. We sincerely hope you will take this opportunity to visit us. Yours sincerely, [Official Site] https://aee.expo-info.jsae.or.jp/en/yokohama/ [DATES] Wednesday, May 27, through Friday, May 29, 2026 10:00 a.m. - 5:00 p.m. [VENUE] PACIFICO Yokohama, Exhibition Hall, Booth No. ２７４ ※Online Exposition Website：Tuesday, May 19, through Tuesday, June 9, 2026

[Speaker] Toshio Fujimura, Ph.D. (Engineering) Representative, Touson Automotive Strategy Research Institute Automotive and Environmental Technology Strategy Analyst Visiting Professor, Aichi Institute of Technology [Former Toyota Motor Corporation] [Lecture Topics] <Chapter 1> Urgent Issue: CO2 Reduction Cannot Wait! <Chapter 2> Achieving a 48% Reduction in CO2 by 2030 Compared to 2019 is Impossible Without Carbon Neutrality in Fuels as Well as Electricity <Chapter 3> Technical Challenges in Electrification <Chapter 4> Pathways to Achieve a 48% Reduction in CO2 Emissions from Owned Vehicles by 2030 <Chapter 5> The Intentions Behind Electrification Strategies of Various Governments (Mainly in Europe, the U.S., and China) <Chapter 6> Electrification Strategies of Major Global Automakers and Sales Trends in Different Regions: Which Manufacturers Will Survive by 2030? <Chapter 7> Conclusion

[Speaker] Masayuki Sawada, Director of Sawada Masayuki Engineering Office [Lecture Topics] 1. The battle in Ukraine is the first large-scale drone war in history. 2. The battle in Iran also sees drones shifting the paradigm of strategy and tactics. 3. Efforts by major European countries (producing drones in Ukraine and collaborating with Ukrainian drone manufacturers). 4. Efforts by the United States (providing intercept drones and other support to Ukraine). 5. Efforts by Taiwan (producing drones in collaboration with U.S. manufacturers that have combat experience in Ukraine). 6. Efforts by Japan's Ministry of Defense (establishing a multi-layered coastal defense system using drones [SHIELD]). 7. Efforts by Japan's Terra Drone Corporation (collaborating with Ukrainian drone manufacturers). 8. Q&A / Business card exchange.