[Case Study] Determination of Insertion Sites for Animal Cultured Cells and Plant Cells

We have added a case study of random integration analysis using the RAISING method!

We would like to introduce a case study of our random integration analysis service using the RAIS method. We performed the RAIS method on T-DNA taglines inserted into Arabidopsis thaliana using the Agrobacterium method, as well as on mouse cultured cells with retrovirus vectors inserted. We confirmed the insertion site sequences using Sanger sequencing and mapped the decoded sequences obtained from next-generation sequencing analysis to the genome sequence. As a result, it was confirmed that this method is effective for determining insertion sites in animal cultured cells as well as in plant cells. 【Random Integration Analysis Service using the RAIS Method】 ■ Sanger Sequencing Plan ■ Next-Generation Sequencing (NGS) Plan * For more details, please refer to the PDF document or feel free to contact us. * For case studies, please refer to the URL below.

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Developed through joint research with the National Institute of Infectious Diseases. Achieves determination of the insertion site of foreign DNA in a short time, with high sensitivity and low cost.

The "RAISING method" is a technique for detecting the insertion sites of foreign DNA in the genome. It allows for rapid and reproducible measurement results with a small amount of DNA. It can identify insertion sites of viral genome insertions, viral vector insertions, searches for fusion genes that could be drug discovery targets, and off-target knock-ins in genome editing, among others. 【Features】 - Analysis can be arranged according to the purpose and target. - Multiple insertion sites can be detected in a single analysis. - High reproducibility of results is achieved through proprietary technology. 【Analysis Examples】 - HTLV-1 (Human T-cell Leukemia Virus) Clonality Analysis Leveraging the characteristics of the RAISING method, which can quickly detect with a small amount of DNA, we achieved high detection sensitivity even with a proviral load of 0.032%. - Off-target Detection of Knock-ins in Genome Editing For knock-in mice created using CRISPR/Cas, we conducted detection of knock-in efficiency and off-target sites by combining with NGS. *For details on examples and services, please refer to the materials available for download.

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In public construction civil engineering sites, there are many instances where sheets are used as common temporary facilities, such as construction roads, access roads, heavy machinery work yards, material storage areas, and worker pathways. Traditionally, steel plates were common, but due to their weight, transportation and installation can be cumbersome. Hyper CLT sheets are civil engineering materials made from wooden CLT for civil engineering use. The 5×10 sheet weighs about 230 kg, and the 5×20 sheet weighs about 460 kg, making them lightweight and easy to transport, install, and remove, even with small machinery. They can be widely utilized for temporary roads, heavy machinery scaffolding, pathways within the site, and protection of material storage in public works. Since they are made of wood, they also contribute to environmental aspects such as reducing summer heat reflection, lowering noise, and fixing CO2. They can simultaneously address construction efficiency, safety measures, and environmental considerations, making them effective for innovative proposals in public works. Furthermore, they improve transportation efficiency and reduce labor in site operations as an alternative to steel plates. Hyper CLT sheets are registered technology with the Ministry of Land, Infrastructure, Transport and Tourism under NETIS "TH-250021-A." They are recommended for companies considering civil engineering, civil engineering use, and civil engineering CLT. Feel free to request materials or consult about implementation. They are temporary materials that also help improve site evaluations in public works. Please consider them.

We have released a new video on our company’s YouTube channel. The theme of this video is "The World of Amino Acid-Based Shampoos." Amino acid-based shampoos have become popular in recent years, but the lather, feel, and finish can vary greatly depending on the cleansing ingredients used. In this video, we clearly explain the changes in user experience based on the differences in cleansing ingredients by comparing two types of amino acid-based shampoos. We hope you can use this as a hint for developing original shampoos and differentiation.

New York and Tokyo, June 26, 2026 - (JCN Newswire) - This year's successful listing of PayPay Corporation (hereinafter "PayPay") on NASDAQ has provided a significant boost. The question now facing Japan's leading innovation companies is no longer "Is a U.S. listing feasible?" but rather "How can we attract global capital and build companies that align with global growth strategies?" The 2nd Japan Go IPO Summit will be held on September 16, 2026, at the Grand Hyatt Tokyo to address this question. This summit is hosted by MarcumAsia and organized by AUM Advisors. To register for this invitation-only event, please apply here: Japan GO IPO Summit Attendee Information https://events.marcumasia.com/view.php?id=32

Our company representative, Shibata, will be speaking as a lecturer at the "Mikata Project" AI Utilization Seminar for the automotive industry, hosted by the Chubu Bureau of Economy, Trade and Industry. ■ Title: AI Implementation Course Leading to Results - Learning Success Patterns and Practical Processes from Real Examples in Manufacturing Sites "We implemented AI, but it is not being used on-site and is left unattended" and "The implementation has become the goal without yielding results" - we have seen many such stumbling blocks in manufacturing sites. In the seminar, based on real examples, we will discuss common patterns for successful implementation that lead to results, pitfalls to avoid, and practical processes to ensure AI is continuously used. We will also propose specific measures for sites struggling with technology transfer and dependency on individuals. ■ Event Overview Date: July 3, 2026 (Friday) 13:30 - 16:00 Venue: TKP Meitetsu Nagoya Station Conference Center + Online Participation Fee: Free / Application Deadline: June 30 (Tuesday) Organizer: Chubu Bureau of Economy, Trade and Industry (Automotive Industry "Mikata Project") Co-presenter: Satoru Hayami, Waseda University For details and registration, please visit the official page of the Chubu Bureau of Economy, Trade and Industry. If you are struggling with AI implementation in manufacturing sites, please feel free to consult us, regardless of region or industry.

July 16 (Thursday) 10:00 AM - 6:00 PM Venue: Convex Okayama Small Exhibition Hall