We have released materials regarding the analysis of the stereoisomers of plasmid DNA pBR322!

Introducing a method for the separation of stereoisomers of plasmid DNA pBR322 using anion exchange chromatography!

In general, plasmid DNA is obtained through purification from E. coli culture broth. The culture broth contains not only contaminating components such as host-derived genomic DNA, RNA, and proteins, but also open circular DNA (Open circular) and linear DNA, which are structural isomers of the target plasmid. Since these can reduce the efficiency of gene expression, clinical plasmid DNA is required to have a purity of over 90% for supercoiled DNA. Therefore, we examined the separation of structural isomers of plasmid DNA pBR322 using anion exchange chromatography, and we would like to introduce this study. *For more details, please refer to the PDF document or feel free to contact us.*

• Analytical Equipment and Devices

The "Mechanical Stitching Method" by Nichinichisui Road Machinery Co., Ltd. is a new technique that allows for the repair of metal cracks without the application of heat. By using special bolts and reinforcement plates, cracks are physically removed, and repairs are made without causing any thermal impact on the base material. Repairs can be carried out in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is minimized. ◎ For more details, please contact us. ◎ If a detailed technical explanation is needed, please contact us.

The "Mechanical Stitching Method" by Nichinichisui Road Machinery Co., Ltd. is a new technique that allows for the repair of metal cracks without the application of heat. By using special bolts and reinforcement plates, cracks are physically removed, and repairs are made without causing any thermal impact to the base material. Repairs can be carried out in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is minimized. ◎ For more details, please contact us. ◎ If you require a detailed technical explanation, please contact us.

The "Mechanical Stitching Method" by Nichinichisui Road Machinery Co., Ltd. is a new technique that allows for the repair of metal cracks without the application of heat. By using special bolts and reinforcement plates, cracks are physically removed, and repairs are made without causing any thermal impact on the base material. Repairs can be carried out even in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is kept to a minimum. ◎ For more details, please contact us. ◎ If a detailed technical explanation is needed, please contact us.

The "Mechanical Stitching Method" by Nichinichisui Road Machinery Co., Ltd. is a new technique that allows for the repair of metal cracks without the application of heat. By using special bolts and reinforcement plates, cracks are physically removed, and repairs are made without any thermal impact on the base material. Repairs can be conducted in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is minimized. ◎ For more details, please contact us. ◎ If a detailed technical explanation is required, please contact us.

We will be exhibiting jointly with Ando Corporation at the "22nd Pipe and Equipment Comprehensive Exhibition 2025 OSAKA," which will be held for three days from September 11 (Thursday) to September 13 (Saturday), 2025, at Intex Osaka, Hall 6A. Please be sure to stop by when you visit. 【Exhibition Schedule】 ● Environmentally friendly heat exchanger "Heat Recovery Heat Exchanger" ● Cut model of "Stainless Steel High Efficiency Heat Exchanger" ● Skeleton model of heating and cooling heat exchanger "SK Water Coil" ● Cooling heat exchanger "SK Water Coil Cartridge Type" ● Radiant heating pipe for space heating "SK Aerofin Tube" ◆ Event Dates: September 11 (Thursday) to September 13 (Saturday), 2025 10:00 AM to 5:00 PM (until 3:00 PM on the last day) ◆ Venue: Intex Osaka, Hall 6A Address: 1-5-102 Nanko-Kita, Suminoe-ku, Osaka City, Osaka Prefecture, 559-0034 Access: https://www.intex-osaka.com/jp/access/ ◆ Booth Number: 112 ◆ Admission Method: Pre-registration required (free) *Please check the official website for details.