Introducing a method for the separation of stereoisomers of plasmid DNA pBR322 using anion exchange chromatography!
In general, plasmid DNA is obtained through purification from E. coli culture broth. The culture broth contains not only contaminating components such as host-derived genomic DNA, RNA, and proteins, but also open circular DNA (Open circular) and linear DNA, which are structural isomers of the target plasmid. Since these can reduce the efficiency of gene expression, clinical plasmid DNA is required to have a purity of over 90% for supercoiled DNA. Therefore, we examined the separation of structural isomers of plasmid DNA pBR322 using anion exchange chromatography, and we would like to introduce this study. *For more details, please refer to the PDF document or feel free to contact us.*
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The BioScience Division is engaged in business in the "Measurement Field," which includes liquid chromatography, columns, and separation agents, and the "Diagnostics Field," which focuses on immunodiagnostics, glycohemoglobin analysis, and genetic testing. In each field, we are expanding our product lineup based on distinctive technologies and establishing sales bases not only in Japan but also overseas for global business development. Additionally, the BioScience Division consistently handles everything from the development, manufacturing, and sales of equipment and reagents to maintenance and customer support, all managed by the Tosoh Group, including its affiliated companies.