Achieving ultra-high performance, miniaturization, and lightweight design! Cell fusion device for cell fusion, nuclear transfer, and oocyte activation.
The "ECFG21" allows for the production of monoclonal antibodies with a fusion efficiency that is 3 to 6 times higher than that of the PEG method. Additionally, it is compatible with large-capacity chamber electrodes (compared to the lower model LF201 cell fusion device). It automatically measures and displays the voltage and current values of AC and DC pulses. 【Applications】 ■ Cell Fusion - Fusion of spleen cells and myeloma cells - Tetraploid rescue method (tetraploid embryo complementation) - Fusion of ES/EG cells with thymus cells (lymphocytes) - Fusion of human dendritic cells and human tumor cells - Fusion of pancreatic islet cells and tumor cells - Production of humanized monoclonal antibodies - Plant protoplast fusion - Liposome droplet fusion - Yeast and mold cell fusion - Mushroom mycelium cell fusion ■ Nuclear Transfer - Somatic cell nuclear transfer with enucleated recipient oocytes - Nuclear transfer of fertilized egg nuclei with enucleated recipient oocytes ■ Oocyte Activation - Electrical stimulation after sperm injection *For more details, please refer to the catalog or feel free to contact us.
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basic information
【Specifications】 ■AC (Alternating Current) ○Waveform: Sine Wave ○AC Voltage: 0–80Vrms (1Vrms increments) ○Frequency: 1MHz ○Time: 0–99sec (1sec increments) ○Post-Fusion: 0–99sec Sine Wave or Damped Wave ○Load Connection: 50Ω or more ■DC Pulse ○DC Waveform: Square Wave ○DC Voltage: 1–1500V (1V increments) ○Pulse Width: 0–99μs (1μsec increments) ○Pulse Interval: 0.1–9.9sec (0.1sec increments) ○Pulse Count: 0–99 times Automatic or Manual ○Damping Rate: 0–99% ■AC (Alternating Current), DC Pulse ○Resistance Measurement: Up to 50.00KΩ ○Setting Value Display: AC, DC, Post-Fusion All Parameters ○Measurement Value Display: AC: Voltage, Current DC: Voltage, Current, Energy ○Memory Function: 99 Conditions ○Measurement Value Display AC: Voltage, Current DC: Voltage, Current, Energy ○Power Supply: 100–240V, 50/60Hz ○Dimensions & Weight: 386W × 370D × 121H mm, 9kg *For more details, please refer to the catalog or feel free to contact us.
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Company information
Neppajine takes pride in contributing to the peace and progress of humanity through biotechnology as a specialized company in life science research equipment, including electroporation (gene introduction using electric pulses) and sonoporation (gene introduction using ultrasound). We believe it is our mission to provide excellent products and information not only within Japan but also to universities, research institutes, hospitals, and private companies around the world. In practice, we have numerous products in use, including gene introduction devices, cell fusion devices, and ultrasound gene introduction devices. We continuously absorb cutting-edge knowledge and technology in biotechnology, engage in close sales activities with our users, provide the latest products, technologies, and information, and respond with a rapid maintenance system. Towards the development of humanity and a bright, happy future, Neppajine will continue to move forward strongly alongside biotechnology.
