Short time, simple, high sensitivity, low-cost DNA insertion site analysis.
It is possible to determine the insertion sites of foreign DNA randomly integrated into the host genome, including the target location. This can be used to identify insertion sites of viral genome, viral vector, fusion genes that may serve as drug discovery targets, and off-target knock-ins in genome editing. Compared to traditional methods for determining transgene insertion sites such as Tail-PCR, LAM-PCR, and Southern blotting, this approach enables analysis that is quicker, simpler, more sensitive, and lower in cost.
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basic information
The RAIS method allows for the design of transgene-specific primers to prepare samples based on PCR. It does not require complicated procedures, eliminating human error in sample preparation, and thanks to its unique technology, it yields highly reproducible results. Analysis arrangements can be tailored according to the purpose and target (both NGS and Sanger sequencing are possible).
Price range
P3
Delivery Time
P4
Applications/Examples of results
It is possible to determine the insertion sites of introduced genes in transgenic organisms and the off-target insertion sites of genome editing knock-ins. It can also be used for the highly sensitive detection of integration into the host genome during gene therapy using viral vectors.
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Company information
In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.