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  6. PCR Master Mix Pipetting Method for Improving qPCR Accuracy

PCR Master Mix Pipetting Method for Improving qPCR Accuracy

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last updated:Jan 17, 2025

ザルトリウス・ジャパン
ザルトリウス・ジャパン
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It is important to have high pipetting skills when dispensing the master mix and to select the appropriate pipette tips.

This document is an application note that introduces guidelines and suitable pipetting methods to maximize the reproducibility of dispensing qPCR master mix. Experimental methods include pipetting techniques, qPCR setup, data analysis, and pre-rinsing of pipette tips before dispensing the master mix. 【Contents (excerpt)】 ■ Introduction ■ Experimental methods and pipetting techniques ■ qPCR setup ■ Data analysis ■ Results *For more details, please refer to the PDF document or feel free to contact us.

    pipette
2020-02-26_14h40_25.png

PCR Master Mix Pipetting Method for Improving qPCR Accuracy

2020-02-26_14h40_25.png
2020-02-26_14h40_25.png
  • Related Link - https://www.sartorius.com/en
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【Other Published Content】 ■ Pre-rinsing of pipette tips before master mix dispensing ■ Low retention tips and standard tips ■ Electric pipettes ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

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PCR master mix pipetting method for improving qPCR accuracy.

PCR master mix pipetting method for improving qPCR accuracy.

APPLICATION_NOTE
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5ml filter-equipped tips with air gap to prevent contamination of pipettes.

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Manual Pipette "mLINE"

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Picus(R) and Picus(R) NxT

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Manual Pipette "Tacta"

Manual Pipette "Tacta"

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Quick Guide to Pipette Cleaning and Contamination Removal Methods

Quick Guide to Pipette Cleaning and Contamination Removal Methods

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ザルトリウス・ジャパン

ザルトリウス・ジャパン

Testing, Analysis and Measurement

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Sartorius is a leading global company that contributes to biopharmaceutical research and the biopharmaceutical industry, established in 1870. Sartorius's laboratory products/services division meets the needs of researchers and technicians engaged in research and development and quality control in the medical industry and various other sectors by providing innovative laboratory equipment and consumables. Our products and technologies are designed to simplify our customers' operations and contribute to accelerating research and improving operational efficiency. *Biotechnology-related products can be found at the following URL: https://premium.ipros.jp/sartorius-stedim/

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