NanoFCM / Flow Nano Analyzer

basic information

■ "Simultaneous discrimination of 'particle size' and 'type' is possible! By equipping the side-scattered light detector with an SPCM (Single Photon Counting Module), high sensitivity is achieved. In addition to measuring particles, it can distinguish between biological and non-biological entities. Through fluorescence measurement, phenotyping (determining phenotype) of labeled biological particles (such as EVs), proteins, nucleic acids, lipids, etc., can be evaluated at the single-molecule level."

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Applications/Examples of results

Phenotyping analysis of extracellular vesicles (EVs) on a single particle basis ■Quantification of EV abundance NanoFCM has fluorescence sensitivity that can detect at the single molecule level. It is possible to measure both endogenous fluorescence and fluorescent labeling for each EV particle, allowing for the differentiation between fluorescent and non-fluorescent EV populations. Proteins, nucleic acids, and lipids can also be evaluated at the single particle level, measuring the relative amount of their presence based on fluorescence signal intensity, enabling phenotyping at the single EV particle level. ■Evaluation of miRNA levels within EVs EVs contain RNA, DNA, proteins, lipids, and metabolites both internally and on their surface, and are likened to vesicle cargo. This EV cargo is transferred to recipient cells, resulting in multifaceted responses. The function of these EVs can be assessed by measuring their composition or evaluating their functionality.