HPLC Method Transfer: Adjustment Function for Gradient Delay Volume
A detailed explanation of one of the most important parameters of method transfer, gradient delay volume.
Transferring methods from old HPLC equipment to new devices or between devices from different manufacturers is a challenging task for many HPLC users. Differences in hardware design, such as the temperature control mode of the column oven, pre-column heater, and specifications of the pump—critical for gradient methods—as well as the design of the auto-sampler and the system volume determined by the plumbing, can affect the results of chromatography. This technical note introduces the gradient delay volume, which is extremely important in method transfer.
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Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.