We provide a highly sensitive binding assay between the SARS-CoV-2 spike protein and ACE2!
Components that inhibit the binding of ACE2 and the novel coronavirus may have the potential to become drugs that prevent viral infection. To conduct this screening, we have prepared the following three types of reagents: - Human ACE2 protein - His tag (HAK-ACE2_UL-1) - SARS-CoV-2 spike protein RBD - Rabbit Fc tag (HAK-SPD_UL-1) - Biotinylated SARS-CoV-2 spike protein RBD - Rabbit Fc tag (HAK-SPD_bio-1)
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basic information
Angiotensin-converting enzyme 2 (ACE2), also known as an ACE homolog, is an endogenous membrane protein that has significant homology with ACE. ACE2 was known to be one of the factors involved in the renin-angiotensin system, which regulates blood pressure. Recently, it has been revealed that the novel coronavirus (SARS-CoV-2), which causes COVID-19, binds to ACE2 present on the cell membrane before being taken up into human cells, suggesting that ACE2 is also a receptor for the novel coronavirus. On our company website (http://www.hakarel.com), we provide detailed binding assay protocols and screening for binding inhibitors using competitive methods on the product introduction page, which can be downloaded and viewed from there. References: (1) Alexandra C. Walls, Young-Jun Park, et al., Cell 180, 1-12 (2020)
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Applications/Examples of results
Human ACE2 protein with His tag (100µg) Usage examples 1. Binding assay The human ACE2 protein with His tag (100µL/well) at concentrations of 0.156-10µg/mL was solid-phase immobilized, and then 0.8µg/mL of biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag was bound to it, detected using HRP-conjugated streptavidin. The linear range of the standard curve in this case was 0.156-1.25µg/mL. 2. Screening of binding inhibitors using a competitive method A binding reaction was conducted with solid-phase immobilized human ACE2 protein with His tag (100µL/well) at 1µg/mL and biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag at 600ng/mL, to which binding inhibitors (SARS-CoV spike protein RBD with rabbit Fc tag) at concentrations of 10-5000ng/mL were added, and detected using HRP-conjugated streptavidin. The 50% inhibitory concentration (IC50) in this case was 140ng/mL (2.7nM). For other usage examples, please refer to our website.
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Company information
Hakarel Co., Ltd. primarily supports research on exosome detection methods and offers contract services such as the production of recombinant proteins, the creation of monoclonal antibodies, the establishment of ELISA measurement systems, exosome ELISA measurements, and proteomics analysis. We respond flexibly to customer requests and provide support for research and development. Please feel free to contact us if you have any inquiries.
