We will introduce examples of off-target detection in knock-in during genome editing!
We would like to introduce a case study of our random integration analysis service using the RAIS method. For a knock-in mouse created using CRISPR/Cas, we attempted to detect the knock-in efficiency and off-target sites by combining the RAIS method with NGS. It was suggested that the donor DNA used for the knock-in was inserted not only into the intended chromosome 9 (chr9) but also into another chromosome. 【Random Integration Analysis Service using the RAIS Method Contents】 ■ Sanger Sequencing Plan ■ Next Generation Sequencing (NGS) Plan *For more details, please refer to the PDF document or feel free to contact us.
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【Flow of Request】 (1) Sample preparation (2) Sample shipment (3) Analysis order (4) Quality check of samples (5) Primer design (6) Sample preparation (7) Sequencing (8) Data delivery *For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.
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