"Improving column efficiency while enhancing the reproducibility of individual sample retention times" by implementing preheating in semi-preparative and preparative HPLC systems.
The Timberline mobile phase heater is used to preheat the solvent in semi-preparative and preparative HPLC systems by controlling the temperature of the solvent. By using heated solvents, the viscosity of the mobile phase is reduced, enhancing column efficiency while improving the reproducibility of retention times for individual samples. This is particularly effective for large compounds such as DNA and RNA, as high-temperature conditions tend to improve mass transfer rates, leading to better peak shapes and separations. 【Features】 ◆ Compact ◆ Safe ◆ Trouble-free 【Specifications】 ■ Control temperature range: Room temperature +5℃ to 90℃ ■ Temperature stability: ±0.1℃ ■ Size ・Heater: 134mmH x 220mmW x 343mmD ・Controller: 83mmH x 204mmW x 229mmD ■ Piping materials: 316SS or Hastelloy C ■ Power supply: 120VAC
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basic information
The mobile phase heater is used to heat the mobile phase in semi-preparative or preparative HPLC. There are many advantages to operating at high column temperatures. It has been shown that operating at high temperatures can reduce the viscosity of the mobile phase, thereby increasing column efficiency. Additionally, controlling the column temperature improves the reproducibility of retention times for individual sample components. Achieving higher column efficiency and maintaining retention time stability leads to higher yields in preparative HPLC systems. The temperature control controller is separated from the heat block, which reduces troubleshooting. The control unit displays the measured values and set values, and can control the temperature inside the coil to within ±0.1°C of the set value. If the temperature exceeds the rating of the fuse, the control unit cuts off power to the heater, protecting the laboratory and column from excessive temperatures.
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