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  6. Products related to the membrane filter method for E. coli testing.

Products related to the membrane filter method for E. coli testing.

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last updated:Oct 18, 2024

Cytiva
Cytiva
  • Official site

Essential for testing E. coli levels in environmental water! The time limit is 12 hours!

In October 2021, the Ministry of the Environment announced the revision of environmental standards related to water quality pollution. As a result of this revision, the standards concerning E. coli have been revised from total coliform counts to E. coli counts. The method for measuring E. coli counts is indicated as the so-called membrane filter (MF) method, which uses a membrane filter with a diameter of 47 mm and a pore size of 0.45 µm. In particular, when measuring E. coli counts, it is necessary to "store the sample in a dark place at 0-5°C (not frozen) if testing cannot be performed immediately after sampling, and it is desirable to test within 9 hours, and within 12 hours at the latest." Therefore, a micro funnel that can be used immediately after opening the bag without the need for prior sterilization is recommended. Cytiva's MicroFunnel integrates a funnel and filter, equipped with a membrane filter of diameter 47 mm and pore size 0.45 µm required for the MF method. The MicroFunnel can be easily detached by simply pinching the cylindrical part, making membrane recovery easy. It can also be connected to standard laboratory manifolds. Each unit is provided in individually packaged, gamma-sterilized form.

    Microbiology
Mic_Micro_Fun100_300_front_Group.jpg

Products related to the membrane filter method for E. coli testing.

Mic_Micro_Fun100_300_front_Group.jpg
Mic_Micro_Fun100_300_front_Group.jpg
  • Related Link - https://www.cytivalifesciences.co.jp/products/pall…

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basic information

【MF Technology of Paul Ensuring Microbial Analysis】 The MF method is an effective technique for analyzing aqueous solutions contaminated by microorganisms and fine particles. It is approved by similar public institutions around the world and is used in various industries for the isolation of specific microorganisms, including E. coli. Compared to other microbial testing methods, the MF method allows for rapid testing of large samples, has high detection sensitivity, and enables the isolation and measurement of dispersed colonies.

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Applications/Examples of results

It can be used for E. coli testing using the MF method. Especially when a large number of samples need to be tested, the sterilized and individually packaged Cytiva MicroFunnel can greatly enhance efficiency. Additionally, since the funnel and filter are integrated, the risk of contamination is also reduced.

Detailed information

  • Mic_Micro_Fun100_300_front_Group.jpg

    Model: 4800 Product Name: MicroFunnel Filter Funnel, 100 mL, GN-6 Metricel, 0.45 µm, White, with Black Grid Product Overview: This is a sterilized, individually packaged micro funnel that combines a funnel and filter into one unit. It features a 0.45 μm cellulose mixed ester membrane certified for microbiological testing, making it suitable for the capture and counting of E. coli.

  • Pall_Lab_Manifold.jpg

    Model: 4889 Product Name: Paul Laboratory Manifold Product Overview: This is a manifold for microbiological testing. It is made of stainless steel, can be disassembled, and all parts can be sterilized, allowing for clean and safe use.

  • MF法.jpg

    The membrane filter method is a highly sensitive technique for accurately quantifying the number of E. coli by filtering environmental water and culturing the captured E. coli on agar medium. The image is a simulation of the MF method experiment.

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Model number overview
4800 MicroFunnel filter funnel, 100 mL, GN-6 Metricel, 0.45 µm, white, with black grid
4889 Pole Laboratory Manifold

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Cytiva

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Cytiva and Pall Life Sciences have merged their businesses and will officially launch under the "Cytiva" brand on May 1, 2023. Under our mission of "Advancing therapies, faster," we will integrate both organizations and focus even more on supporting the development and manufacturing of better therapies for patients as a stronger global provider of technologies and services. The new Cytiva will offer a broader and deeper expertise and a rich lineup of products and services to better support various customers involved in research and the manufacturing and development of biopharmaceuticals, enabling them to deliver therapies to patients more quickly, from discovery to delivery. With a rich history spanning several hundred years, Pall Life Sciences and Cytiva, as part of the Danaher Group, will continue to unite to provide better services to our customers.

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