[Analysis Case] Characterization of Glycosylated Proteins (LC-TOFMS)
It is possible to efficiently advance the development and quality assurance of biopharmaceuticals!
The LC-TOFMS (Liquid Chromatography Time-of-Flight Mass Spectrometry) method is useful for the characterization analysis of proteins (such as molecular weight analysis, peptide mapping, and glycan analysis). We will introduce a case study of the analysis and examination of the glycan (glycoform and glycan attachment sites) of IgG antibodies using the LC-TOFMS method. ■Analysis Sample: IgG Antibody ■Analysis Method: LC-TOFMS ■Analysis Results - Glycoform Analysis By analyzing the molecular weight, it is possible to estimate the glycoforms of glycoproteins. LC-TOFMS measurement of the IgG antibody revealed the presence of five different glycoforms. - Glycan Attachment Site Analysis The IgG antibody was fragmented through enzymatic treatment and measured by LC-TOFMS. The analysis of the measurement results using dedicated software showed that a N-linked glycan (G0F, G1F, G2F) was attached to the asparagine of the peptide fragment with the amino acid sequence EEQFNSTFR. *For detailed results, please refer to the PDF document.
Inquire About This Product
basic information
For more details, please refer to the PDF document or feel free to contact us.
Price range
Delivery Time
Applications/Examples of results
For more details, please refer to the PDF document or feel free to contact us.
catalog（2）
Download All Catalogs
Company information
Kaneka Techno Research Co., Ltd. was established in 1988 with the corporate philosophy of becoming a trusted partner for our customers in the fields of "analytical services" and "technical information services." As a group of professionals with foresight and advanced analytical and research techniques, we aim to propose solutions to the challenges faced by our customers and to provide high-quality technical services quickly, thereby becoming a trusted problem-solving technical service company. We will continue to strive for improvement every day. We sincerely request your continued guidance and support.
![[Analysis Case] Characterization of Culture Medium](https://image.mono.ipros.com/public/product/image/95e/2000656034/IPROS93531127289564224896.png?w=280&h=280)
![[Analysis Case] Analysis of Nitrosamines (LC-MS/MS Analysis)](https://image.mono.ipros.com/public/product/image/1e6/2001167027/IPROS83636395334605863414.jpeg?w=280&h=280)
![[Analysis Case] Quantitative Analysis of CO2 Emitted During Cement Heating](https://image.mono.ipros.com/public/product/image/8c0/2001177429/IPROS75238002221992873515.jpeg?w=280&h=280)
![[Analysis Case] Analysis of the Thermal Decomposition Behavior of Polyimide (TG-MS)](https://image.mono.ipros.com/public/product/image/ffb/2001178436/IPROS91512049690560525295.jpeg?w=280&h=280)
