[Antibody Craftsman Case] Successful Case of Antibody Distinguishing Specific Oxidized Cys

Production of antibodies through selection "in vitro" without animal immunity and verification of the specificity of the obtained antibodies.

Among the three Cys residues present in the DJ-1 protein, which is said to be involved in Parkinson's disease, we attempted to obtain antibodies that specifically recognize only the oxidized DJ-1 with the 106th Cys. This was done through the production of polyclonal antibodies via immunization in rabbits and monoclonal antibodies via immunization in mice, but we were unable to obtain the desired antibodies. Therefore, we used a peptide synthesized based on the surrounding sequence that includes the oxidized C106 as the antigen, and created conjugates with different carrier proteins and cross-linkers. As a result, we were able to identify clones that showed positive results for the oxidized C106 peptide and negative results for the reduced C106 peptide. [Case Summary] <Problem> ■ The methods of producing polyclonal antibodies through immunization in rabbits and monoclonal antibodies through immunization in mice did not yield the desired antibodies. <Result> ■ We were able to identify clones that showed positive results for the oxidized C106 peptide and negative results for the reduced C106 peptide. *For more details, please refer to the PDF document or feel free to contact us.

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