Genetic analysis of microorganisms
This is a test to extract DNA from a sample (culture), determine part of the nucleotide sequence of the 16S rRNA gene region (bacteria) or ITS region (fungi), and obtain information to identify the species of microorganisms.
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basic information
Price information
14,000 yen per sample (excluding tax) *If analyzing almost the entire region of the 16S rRNA gene (using 10F and 1500R), the cost will be 17,000 yen per sample (excluding tax). *For re-analysis (if changing the analysis region or if the sample is re-cultured by our company for retesting), an additional fee of 12,000 yen per sample (excluding tax) will apply.
Price range
P2
Delivery Time
P3
※After the sample arrives at our company, it will take about 5 business days (business days are only weekdays).
Applications/Examples of results
【Testing Procedure】 1. DNA Extraction Collect the microbial cells from the sample (culture) and extract the DNA. If the sample has insufficient isolation (pure culture), there is a possibility that sequence data may not be obtained. ↓ 2. PCR Amplify the specified analysis region (16S rRNA gene region or ITS region) using PCR. For the 16S rRNA gene region, amplification is generally performed using 10F and 800R (refer to 【used primers】, approximately 0.8 kb of the 16S rRNA gene region). If you wish to analyze almost the entire region (using 10F and 1500R), please let us know. ↓ 3. Sequencing Reaction Perform a sequencing reaction using the products amplified by PCR. ↓ 4. Capillary Electrophoresis Obtain sequence data by separating and detecting the products of the sequencing reaction using capillary electrophoresis.
Company information
At our center, we not only conduct tests as specified by JIS and other regulations, but we also propose testing methods that suit the purpose of the tests. We capture the entire problem for problem-solving and provide practical advice.
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