Disinfection performance testing method for wet wipes.
This is the disinfection performance testing method for wet wipes by the Japan Hygiene Materials Industry Association.
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basic information
Using wet wipes, wipe the test carrier inoculated with the bacterial solution according to the specified method, and examine the number of viable bacteria on the test piece. Bovine serum albumin is added to the bacterial solution as a model contaminant.
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Applications/Examples of results
**Testing Method** 1. Sample Preparation (3 pieces each) Prepare as follows: - Test Sample: The specimen (wet wipe) itself. It is also possible to create and use a liquid-soaked cotton cloth. - Control Sample: A cotton cloth (Cotton No. 3-1, 15 cm × 10 cm) soaked in 1.5 times the amount of phosphate buffer solution. 2. Application of Test Bacterial Solution to Test Carrier Apply the test bacterial solution (prepared in normal broth medium at 1.0 to 5.0 × 10^9 CFU/mL mixed in equal amounts with bovine serum albumin solution (model stain)) to the test carrier (stainless steel piece). 3. Wiping of Test Carrier with Sample Attach one piece of the sample to a 150 g weight, then press it against the test carrier installed in a dedicated wiping device, and perform 5 back-and-forth motions at a rate of 120 times/minute. 4. Measurement of Viable Bacterial Count Wash out the test carrier and measure the viable bacterial count. 5. Evaluation Compare the logarithmic values of the remaining viable bacterial counts on the test carrier after wiping with the test sample and control sample, and calculate the antibacterial activity value.
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At our center, we not only conduct tests as specified by JIS and other regulations, but we also propose testing methods that suit the purpose of the tests. We capture the entire problem for problem-solving and provide practical advice.
