Unique Antibody Production Technology - HuCAL Technology
HuCAL technology is an antibody production technique that does not rely on animals and conducts all selections in vitro. Specifically, antibodies are presented using phages that incorporate human antibody genes, allowing them to bind to antigens. Since the presented antibodies (Fab antibodies) correspond one-to-one with the genes that code for them in the form of phage particles, it is easy to identify the genes of the Fab antibodies that bind to the antigen from among many Fab antibodies (phage display). A solution containing phages is added to a plate with immobilized antigens, allowing the phages to bind through the presented antibodies. Phages that did not bind to the antigens are washed away, and the bound phages are then eluted and collected. By repeating this process several times, phages that bind more strongly to the antigen can be concentrated (panning). The antibody genes encoded by the phages obtained through panning are expressed in E. coli, and Fab antibodies are purified. *For more details, please refer to the PDF materials or feel free to contact us.*
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basic information
▼First, from Planning▼ The "Antibody Craftsman" monoclonal antibody production service begins with planning when we receive inquiries about antibody production from our customers. To create antibodies suitable for the customer's intended use, we ask about the characteristics of the antigen and the purpose of the antibody. Next, we design the antigen according to the purpose of the antibody to be obtained and propose strategic selection methods tailored to the type of antigen and the application of the antibody, guided by experienced personnel throughout the entire process. ▼Format and Reproduction of Delivered Antibodies▼ The antibodies produced will be in the form of Fab, and you can choose between monovalent or divalent options, as well as two types of tags, to be delivered in your desired format. The resulting antibodies are purified as recombinant proteins from E. coli, allowing us to deliver them with the same quality repeatedly. Additionally, it is easy to convert Fab into immunoglobulin isotypes such as IgA, IgE, IgG, and IgM.
Price range
Delivery Time
※At the shortest, 7 weeks from the receipt of the antigen.
Applications/Examples of results
Antibodies produced by "Antibody Craftsman" can be used in various applications just like antibodies produced by conventional methods. Furthermore, since they are Fab antibodies, they possess properties that can provide additional advantages in certain assay systems, such as not needing to remove the Fc region to prevent binding to receptors or to minimize the reaction of human anti-mouse antibodies (HAMA). Additionally, tag-specific detection antibodies are very useful in many applications.
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Company information
Gene Frontier has been expanding its business areas that contribute to the acceleration of biopharmaceuticals, including antibody drugs, and has been advancing the development of its unique technologies. Moving forward, we aim to accelerate our research and development and business development to achieve further growth on a global scale. We will continue to provide unique technologies and services for the development of innovative pharmaceuticals and diagnostic drugs, striving to be a trusted partner for our customers and contribute to 21st-century healthcare.