5 x TBE

A buffer consisting of Tris, boric acid, and EDTA! A buffer commonly used during electrophoresis.

The "5 x TBE" is a stock solution at a concentration five times the usage conditions. It is a buffer composed of Tris, boric acid, and EDTA, commonly used during electrophoresis, similar to TAE (10 x TAE, 50 x TAE). When used in agarose gel electrophoresis, it can be diluted to "0.5 x TBE" with water at a 10-fold dilution. For polyacrylamide gel electrophoresis, "1 x TBE" is used, diluted 5 times with water. 【Specifications】 ■ Composition: 0.445 mol/l Tris-borate, 10 mmol/l EDTA ■ Dilution Guidelines - Agarose Gel Electrophoresis: 10-fold dilution (Working Solution: 0.5 x TBE) - Polyacrylamide Gel Electrophoresis: 5-fold dilution (Working Solution: 1 x TBE) ■ Storage Method: Room temperature ■ Notes: Confirm that normal electrophoresis can be performed without issues. *For more details, please refer to the related links or feel free to contact us.

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