Nucleic acid analysis HPLC buffer 'TEAA Buffer A'
ADS Biotec / 553421
TEAA buffer for nucleic acid analysis using high-performance liquid chromatography (HPLC), which can also be used for mRNA analysis and purification.
TEAA Buffer A is an aqueous solution of 0.1M triethylammonium acetate (TEAA). When used in conjunction with TEAA Buffer B in a gradient, it enables the analysis and purification of mRNA and nucleic acids.
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basic information
TEAA Buffer A, manufactured by ADS Biotec (formerly Transgenomic), is a buffer for nucleic acid analysis using ion-pair reverse phase HPLC. When used in conjunction with TEAA Buffer B in a gradient method, it enables high-precision separation of nucleic acids. The composition is a 0.1M aqueous solution of triethylamine acetate (TEAA). It is the optimal buffer for columns using alkylated polystyrene-divinylbenzene (PS-DVB) beads produced by ADS Biotec, and it is recommended to be used together with the column. This product is adjusted to a concentration suitable for analysis and purification, reducing the time and effort required to prepare the buffer, thereby contributing to improved productivity and stabilization of experimental conditions. It has been cited in numerous papers in the field of nucleic acids. It can be used for the analysis and purification of mRNA, making it suitable for research and development of mRNA vaccines and mRNA therapeutics.
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Applications/Examples of results
Nucleic acid analysis, mRNA analysis, mRNA purification.
Detailed information
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Sample: RNA ladder (0.2 – 6kb) Flow rate: 0.9 mL/min Temperature: 75℃ Mobile phase: TEAA Buffer A (0.1M TEAA) TEAA Buffer B (0.1M TEAA, 25% Acetonitrile) Experimental method: Analysis was performed using a gradient of Buffer A and Buffer B. The concentration of Buffer B was changed from 38% to 55% over more than 30 minutes.
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ADS Biotec (formerly Transgenomic) is based in Omaha, Nebraska, USA, and Glasgow, UK, and has been providing equipment, consumables, and services to customers in the fields of cytogenetics, pathology, and molecular genetics for over 20 years. The Omaha facility is equipped for the manufacturing, storage, and quality control of reagents, and conducts thorough quality management based on ISO 13485.
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Shorten the time and effort required for experiments. At ADS Biotec, we sell buffers that are pre-adjusted for concentration. This allows you to reduce the time and effort needed to create buffers, thereby improving the productivity of your experiments.
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ADSTEC Corporation has been committed to customer-oriented principles since its establishment in 2002, focusing on two main businesses: the import and sale of image-related equipment, and the development and manufacturing of gene-related equipment. We take pride in having established a system that allows us to respond to our customers' needs in a detailed and speedy manner, expanding our business network both in Japan and overseas in this rapidly evolving field. In these two areas, we aspire to be a group of experts who can assist with projects, including customer proposals, and we are dedicated to enhancing our technical skills as well as investing in staff education to cultivate "human power." Moving forward, we will continue to assess the trends of the times and provide and develop products that truly match our customers' needs.