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  6. Evaluation method for the destruction and removal of biofilms.

Evaluation method for the destruction and removal of biofilms.

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last updated:Oct 02, 2025

同仁グローカル
同仁グローカル
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Development of agents to effectively destroy and remove biofilms is underway!

We would like to introduce our "Evaluation Method for the Destruction and Removal Effects of Biofilms," which is part of the biofilm-related analysis we conduct. When evaluating the function of cleaning agents that destroy and remove formed biofilms, we generally use a 96-well plate to allow biofilms to form on each well. The biofilms are then immersed in the cleaning agent for a specified period, and the amount of biofilm removal is compared against sterilized water (or sterilized buffer, etc.) as a control. 【Analysis Method】 1. Immerse the substrate or pins in a bacterial culture solution and incubate at a constant temperature for 24 to 48 hours to allow biofilm formation on the substrate or pins. 2. After immersing the substrate or pins in the biofilm cleaning agent for a specified time, rinse with water. 3. Stain the substrate or pins with crystal violet. 4. Extract the crystal violet and measure the absorbance at 590 nm. 5. Compare the biofilm amounts between the control and the samples. *For more details, please refer to the PDF document or feel free to contact us.

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Evaluation method for the destruction and removal of biofilms.

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  • Related Link - https://www.dojin-glocal.com/biofilm

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Evaluation method for the destruction and removal effects of biofilms

Evaluation method for the destruction and removal effects of biofilms

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In addition to our environmental analysis business, we conduct a wide range of contract analysis as a partner for researchers and engineers who "support science." We have started to offer services for measuring antioxidant capacity in functional foods (using SOD-like activity measurement kits and the DPPH method), analyzing enzyme activity and enzyme inhibition activity, and various fatty acid analyses. Recently, we have begun contract analysis for measuring biofilm formation and inhibition, as well as assessing the effects of biofilm agents, continuously challenging ourselves in new areas.

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