Evaluation method for the destruction and removal of biofilms.

Development of agents to effectively destroy and remove biofilms is underway!

We would like to introduce our "Evaluation Method for the Destruction and Removal Effects of Biofilms," which is part of the biofilm-related analysis we conduct. When evaluating the function of cleaning agents that destroy and remove formed biofilms, we generally use a 96-well plate to allow biofilms to form on each well. The biofilms are then immersed in the cleaning agent for a specified period, and the amount of biofilm removal is compared against sterilized water (or sterilized buffer, etc.) as a control. 【Analysis Method】 1. Immerse the substrate or pins in a bacterial culture solution and incubate at a constant temperature for 24 to 48 hours to allow biofilm formation on the substrate or pins. 2. After immersing the substrate or pins in the biofilm cleaning agent for a specified time, rinse with water. 3. Stain the substrate or pins with crystal violet. 4. Extract the crystal violet and measure the absorbance at 590 nm. 5. Compare the biofilm amounts between the control and the samples. *For more details, please refer to the PDF document or feel free to contact us.

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