Leveraging Sierra's high throughput, we established an accurate evaluation of slow dissociation antibody-antigen interactions using the SICK method and high-sensitivity HCP quantification through SPR.
The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control. **Abstract** The surface plasmon resonance (SPR) method is gaining attention for the analysis and evaluation of intermolecular interactions and quantification. The Sierra SPR-24Pro (Bruker) features 24 sensor spots, enabling high-throughput and rapid measurements from optimal condition exploration to evaluation of multiple samples. This article introduces Sierra SPR's unique kinetics analysis technology that does not use regeneration solutions, and presents kinetics analysis results of antibodies that exhibit very strong affinity, which have traditionally been difficult or time-consuming to analyze. Additionally, we report the development of a highly sensitive quantification method for Host Cell Proteins (HCP) using SPR. **Table of Contents** 1. Introduction 2. Kinetics Analysis 3. Quantification of HCP 4. Conclusion
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Technical Information Magazine The TRC News "Kinetics Analysis and Quantification Method Using Surface Plasmon Resonance" [Abstract] Surface plasmon resonance (SPR) is gaining attention for the analysis and evaluation of molecular interactions and quantification. The Sierra SPR-24Pro (Bruker) features 24 sensor spots, enabling high-throughput and rapid measurements from optimal condition exploration to evaluation of multiple samples. This article introduces Sierra SPR's unique kinetics analysis technology that does not use regeneration solutions, and presents kinetics analysis results for antibodies that exhibit very strong affinity, which have traditionally been difficult or time-consuming to analyze. Additionally, we report the development of a highly sensitive quantification method for Host Cell Proteins (HCP) using SPR. [Table of Contents] 1. Introduction 2. Kinetics Analysis 3. Quantification of HCP 4. Conclusion [Figures and Tables] Figure 1 SPR Method Overview Figure 2 Injection of Sierra SPR-24 Pro Figure 3 SPR Measurement (MICK Method) Figure 4 SPR Measurement (TCK Method) Figure 5 SPR Measurement (SICK Method) Figure 6 SPR Measurement of HCP Standard Sample Figure 7 Calibration Curve and Recovery Rate
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At Toray Research Center, Inc., we provide technical support through analytical techniques and physical property analysis to address various challenges in research and development as well as production fields via contract analysis. Based on our long-standing achievements and extensive experience in analysis and physical property evaluation, we continue to strive to meet the advanced and diverse needs of our customers.