An explanation of determining the purity ratio of plasmid DNA in human gene therapy products using slope spectroscopy!
This document is an application note for "Solo VPE." The measurement of DNA purity in gene therapy plasmids in the presence of proteins can be assessed using the UV absorbance ratio of 260/280. However, this method has challenges when accurate quantification of DNA purity is required. Our product and its slope spectrophotometry offer a new method to accurately measure DNA purity ratios and overcome these challenges. [Contents] ■Summary ■Apparatus/Equipment ■Method & Results ■Analysis ■Conclusion ■References *For more details, please download the PDF or feel free to contact us.
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Repligen (hereinafter referred to as our company) is a life sciences company specialized in bioprocessing, providing over 35 years of expertise and innovation to our customers. We promote advancements in bioprocessing through products that address critical steps in the manufacturing of biological products, primarily monoclonal antibodies, and through the development and commercialization of flexible solutions to our customers' challenges. Our products are utilized in filtration, separation, purification, and concentration operations in pharmaceuticals, clinical analytical reagents, food, beverages, and industrial process liquids. Our manufacturing facilities are located in Waltham, Massachusetts, USA; Lund, Sweden; Waiblingen, Germany; Rancho Dominguez, California, USA; and Shrewsbury, Massachusetts, USA. Additionally, we sell our products globally through strategic partners in the United States, Europe, and Asia.