2D-LC-CAD analysis of lactose and maltose in sugar-containing foods.
[Case Study Introduction] Using the single-loop heart cut 2D-LC method, it is possible to quantitatively analyze main components and trace components with high sensitivity and accuracy.
Foods containing sugar often have a high content of sucrose, making it difficult to accurately quantify sucrose and other disaccharides (such as lactose and maltose) in actual samples due to insufficient separation. This technical document adopts a single-loop heart-cut 2D-LC method, where lactose and maltose are fractionated in the sample loop after sucrose elutes in the first dimension, allowing for the separation and analysis of lactose and maltose in the second dimension, thereby avoiding the influence of sucrose. Additionally, the charged aerosol detector (CAD) used in the detection allows for gradient analysis, providing greater flexibility in separating contaminants in a variety of food samples, enabling efficient analysis. This achieves high-sensitivity analysis that was difficult with conventional refractive index detectors (RI). 【Features】 - Accurate analysis by separating lactose and maltose in foods containing sugar - Separation from contaminants possible with the single-loop heart-cut 2D-LC method - Charged aerosol detector (CAD) allows for gradient analysis and accommodates diverse samples - Achieves high-sensitivity analysis that is difficult with conventional refractive index detectors (RI)
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basic information
Features of charged particle detectors: - 10 to 100 times higher sensitivity than RI - Weight-dependent response that does not depend on the chemical structure of the analyte - Useful for detecting non-volatile and semi-volatile substances that do not absorb UV light and are difficult to detect by mass spectrometry due to low ionization efficiency
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There are implementation results in food manufacturers, contract analysis organizations, and research institutions. In particular, it is highly regarded as a technology that enables high-sensitivity analysis, which was difficult with conventional differential refractometers (RI).
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Company information
Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.
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