Application Report: How to Optimize Records in Cardiac Electrophysiology

Describing how to optimize recordings in cardiac electrophysiology using the Qube 384 perforated patch clamp.

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have the potential to revolutionize heart disease modeling and drug screening. However, capturing physiologically relevant action potentials (APs) has been challenging, especially with traditional whole-cell (WC) patch-clamp methods. WC recordings often disrupt the cellular environment and can shorten AP duration due to the "washing out" of cytoplasmic components. By using perforated patch-clamp techniques, it is possible to maintain electrical access while preserving cell integrity. Unlike WC, the perforated method uses agents like nystatin to create small pores in the cell membrane, allowing ions to pass while retaining important cytoplasmic components. Applying this method has significantly improved AP recordings on the Sophion Qube 384 platform, demonstrating a success rate of up to 40%. *For more details, please refer to the related links. Feel free to contact us for further information.*

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