[Case Study] AAV Titer Measurement After Downstream Process
By adopting size exclusion chromatography and highly sensitive fluorescence detection, accurate and reproducible measurements can be achieved with a small amount of sample!
We introduce a rapid and cost-effective method for measuring the titer of adeno-associated virus (AAV) products after downstream processing, combining size exclusion chromatography (SEC) with a highly sensitive fluorescence detector. By using the Thermo Scientific MAbPac SEC-1 column, efficient separation of AAV capsids and low molecular weight impurities is achieved, allowing for detailed monitoring of product quality attributes (PQA). This method enables rapid determination of AAV product titer after a single step of affinity purification, contributing to the evaluation of product characteristics. 【Features】 ■ Rapid and cost-effective titer measurement method ■ Measurement possible with small sample sizes due to high-sensitivity fluorescence detection ■ Efficient separation using the MAbPac SEC-1 column ■ Detailed monitoring of PQA is possible ■ Improved reproducibility with low-adsorption glass vials (RSD less than 5%) *For more details, please download the PDF or feel free to contact us.
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basic information
【System Configuration】 Thermo Scientific Vanquish Flex UHPLC System - Pump: Thermo Scientific Vanquish Quaternary Pump F - Autosampler: Thermo Scientific Vanquish Split Sampler HT - Column Oven: Thermo Scientific Vanquish Column Compartment H - Detector: Thermo Scientific Vanquish Fluorescence Detector F with Dual PMT Software - Thermo Scientific Chromeleon Chromatography Data System 7.2.10
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For more details, please download the PDF or feel free to contact us. It can only be used for research purposes and cannot be used for diagnostic purposes.
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Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.
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