[Analysis Case] Cryo-SEM Observation and Elemental Analysis of Cosmetics

We will introduce an example of observing the morphology of fine particles contained in a makeup primer using the cryo-SEM method.

Basic cosmetics such as lotions and serums, as well as beauty cosmetics like lipsticks and foundations, are multi-component mixtures of water, oils, emulsifiers, pigments, and more. Since the fine structure and dispersion state of these components affect the appearance and finish during application, it is important to verify the state of each component. The cryo-SEM method is a technique that allows for the observation of fine structures and dispersion states while maintaining a paste-like structure. After freezing and fracturing the primer, we conducted morphological observations of the fine particles contained in the primer using the cryo-SEM method. Additionally, we performed elemental mapping analysis using the SEM-EDX method to confirm the distribution of each element. [Analysis Sample] Primer [Analysis Method] The primer was rapidly frozen as is, and an observation surface was prepared through freeze-fracturing, followed by cryo-SEM observation and elemental analysis using the SEM-EDX method. (Freeze-fracturing: Leica Microsystems ACE600FF, Cryo-SEM: Zeiss EVO15LS) (EDX: Bruker AXS QUANTAX XFlash6|30) By combining the cryo-SEM method and the SEM-EDX method, it is possible to confirm the distribution of elements even in fluid samples.

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