[Analysis Case] Simple Quantitative Analysis of Sugars

This is an example of a simple quantitative analysis of seven types of sugars converted to glucose equivalents using an evaporative light scattering detector (ELSD).

Sugars come in many types, and quantitative analysis to determine the sugar content in samples using standard substances for each type can become complicated, involving various tasks from pretreatment to measurement and analysis. Here, we introduce a case where an evaporative light scattering detector (ELSD) was used to simply quantify seven types of sugars by converting them to glucose equivalents. 【Analysis Samples】 Standard substances: 7 types of sugars (galactose, glucose, sucrose, trehalose, fructose, maltose, lactose) Samples: Commercial vegetable juices A and B, lactic acid bacteria drinks 【Analysis Method】 A mixture of the seven types of sugar standard substances was prepared, and HPLC-ELSD measurement was conducted. Using the conversion quantitative method based on glucose equivalents allows for a straightforward quantification of sugars in the samples. This method is effective when wanting to confirm the approximate concentration of sugars contained in various samples related to bio-manufacturing, such as food and beverages, cell and microbial culture media, and culture solutions.

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