Rapid Microbiological Method (RMM) by Imaging
CFU (Colony Forming Unit) Measurement for Quality Control and Safety Inspection ○ Overwhelming Speed: The testing that traditionally took 3 days for results has been dramatically shortened to 3-4 hours (initial detection) and 6-10 hours (final concentration confirmation). ○ High-Precision Scanning Technology: Utilizing a high-resolution 3D optical scanning system, it can detect colonies as small as 8 µm in diameter. ○ No Change to On-Site Operations: Traditional culture temperatures, liquid volumes, and media can be used as is. It is very easy to correlate with official methods since microorganisms are detected and counted as CFUs while still alive, and identification of the bacteria after detection is also possible. ○ Compliant with FDA 21 CFR Part 11.
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basic information
The patented high-resolution 3D optical scanning system is designed to optimize both image quality and quantitative data. It can detect a wide range of objects from 0.5 µm to 2 mm, covering all types of microorganisms. It is compatible with standard 12-well and 6-well microplates. The footprint of the main unit is very compact and space-saving at 450 × 260 × 250 mm (height when the lid is open: 550 mm), allowing for flexible operation according to the environment. ○ System Specifications - Operating temperature: 5 to 40°C - Operating humidity: 20 to 93% RH - Dimensions (L × W × H): 450 × 260 × 250 mm (height when the lid is open: 550 mm) - Weight: 9.6 kg - Power adapter: Input 100-240 VAC, 50/60 Hz / Output 24V DC, 2000 mA - Power consumption: Standby 11 W / Operating 20 W The device is typically used installed within an incubator, but it is also possible to configure a system with a built-in mini incubator as described below.
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Applications/Examples of results
Quality Control and Safety Inspection for Microbial Contamination ○ Bio-burden Testing → CFU (Colony Forming Units) can be calculated in just a few hours. Supports quick identification of causes and appropriate corrective actions when microbial contamination outside specifications or abnormal trends are detected. ○ Industrial Water Testing → Detectable from just 1 CFU per 100mL water sample. Accurately monitors the presence of microorganisms in industrial water. ○ Food and Beverage Testing → By placing the inspection system close to critical control points (CCP) in the production area, it accurately monitors microbial loads in the most critical locations. ○ Cell Culture → Accurately detects contamination in mammalian cell cultures, supporting optimal biopharmaceutical manufacturing. ○ Environmental Monitoring → Minimizes equipment downtime associated with identifying contamination sources in the manufacturing environment, enabling faster production resumption.
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Company information
Neppajine takes pride in contributing to the peace and progress of humanity through biotechnology as a specialized company in life science research equipment, including electroporation (gene introduction using electric pulses) and sonoporation (gene introduction using ultrasound). We believe it is our mission to provide excellent products and information not only within Japan but also to universities, research institutes, hospitals, and private companies around the world. In practice, we have numerous products in use, including gene introduction devices, cell fusion devices, and ultrasound gene introduction devices. We continuously absorb cutting-edge knowledge and technology in biotechnology, engage in close sales activities with our users, provide the latest products, technologies, and information, and respond with a rapid maintenance system. Towards the development of humanity and a bright, happy future, Neppajine will continue to move forward strongly alongside biotechnology.
