On-Demand Video: Introduction to Spatial Biology Antibodies: Important Considerations and Validation Methods
Invitrogen EVOS S1000 Spatial Imaging System
A webinar that systematically explains the fundamentals of spatial proteomics technology and its practical applications.
This presentation will introduce an overview of spatial proteomics, which has been gaining attention in recent years, as well as the important role antibodies play in immunohistochemistry (IHC) and multiplex staining analysis. It will also discuss the essential concepts of antibody validation necessary for obtaining reliable data. Additionally, it will touch on fluorescent dyes, labeling techniques, and the optimization of analysis workflows, providing specific tips for common challenges faced in research settings. In the latter half, we will mention our latest imaging systems and related reagents, explaining practical insights for efficiently and accurately advancing spatial analysis. This content will be beneficial for a wide range of individuals, from those looking to introduce spatial analysis techniques to researchers who are already utilizing them. Recommended for: - Those who want to learn the fundamentals of spatial proteomics technology. - Researchers who feel challenged by antibody selection and validation in IHC and multiplex staining. - Individuals looking to optimize fluorescent labeling and analysis workflows. - Those interested in the latest imaging technologies and reagent information for spatial analysis. - Life science technicians and researchers who want to enhance the reproducibility and reliability of research data.
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basic information
Spatial biology is a research field aimed at understanding the distribution and interactions of single cells within tissues, providing important information for deciphering disease progression and treatment responses. The video compares the characteristics and challenges of traditional multiplex fluorescent imaging, cyclic imaging, and spectral imaging, with a detailed introduction to the advantages of spectral imaging in particular. Furthermore, it focuses on spatial proteomics analysis using the Invitrogen EVOS S1000 Spatial Imaging System, explaining its fast and convenient workflow that can acquire up to 9-plex in a single measurement, the automation of spectral unmixing, and reliability assessment through quality indicator reports. Additionally, through specific case studies utilizing Invitrogen Aluora spatial amplification reagents and IHC-validated primary antibody conjugates, it presents examples of visualizing and analyzing the tumor microenvironment in cancer tissues, demonstrating the potential for application in research settings.
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P7
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Applications/Examples of results
For research use only. Not for diagnostic use. *For more details, please refer to the PDF materials or feel free to contact us.*
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Company information
Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.
