What is reverse phase liquid chromatography? Free explanatory materials available.
What is reverse phase liquid chromatography? A thorough and easy-to-understand explanation of its differences from normal phase, with free basic knowledge materials available!
Reverse phase liquid chromatography uses a packing material with a low-polarity chemically bonded phase as the stationary phase and a high-polarity solvent as the mobile phase. High-polarity compounds are less retained by the stationary phase and elute quickly, while low-polarity compounds elute more slowly. Octadecylsilyl silica gel (ODS), which incorporates octadecyl groups (C18) into silica gel, is a widely used packing material in HPLC. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are new to HPLC, want to review from the basics, or are looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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