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Agilent BioSEC-3 LC column for biopharmaceutical analysis

Agilent BioSEC-3 LC column for biopharmaceutical analysis

Compatible with size exclusion analysis of most peptides and proteins!

The "Agilent BioSEC-3" is an LC column for biopharmaceutical analysis that realizes innovative technology in size exclusion chromatography (SEC). It is packed with 3μm silica particles coated with a unique hydrophilic layer. This thin polymer layer is chemically bonded to pure silica, which has sufficient mechanical strength under controlled conditions, resulting in very high separation efficiency in size exclusion. 【Features】 ■ Outstanding capacity, stability, and reproducibility for size-based separation of biomolecules ■ Sharper peaks, higher resolution, and excellent protein recovery rates ■ Achieves faster and higher separation than conventional SEC (size exclusion) columns ■ Compatible with most aqueous buffers ■ Excellent stability under both high salt and low salt conditions *For more details, please refer to the PDF document or feel free to contact us.

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Column "Agilent Bio Ion Exchange"

Column "Agilent Bio Ion Exchange"

A wide lineup of 1.7, 3, 5, and 10μm particles in all sizes!

"Agilent Bio Ion Exchange" is an LC column for biopharmaceutical analysis filled with non-porous ion exchange polymer particles. It is designed to achieve high resolution, high recovery, and high-efficiency separation of peptides, oligonucleotides, and proteins. There are strong cation exchange (SCX), weak cation exchange (WCX), strong anion exchange (SAX), and weak anion exchange (WAX) phases. [Features] - Rigid non-porous poly(styrene-divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic polymer layer to eliminate non-specific binding. - Uniformly filled ion exchange functional groups are chemically bonded to the hydrophilic layer (with multiple ion exchange groups per anchoring), increasing column capacity. - The particles, coating, and bonding are resistant to high pressure, promoting higher resolution and faster separation. *For more details, please refer to the PDF document or feel free to contact us.

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"Poroshell 300" LC column for biopharmaceutical analysis

"Poroshell 300" LC column for biopharmaceutical analysis

In addition to protein separation for analysis, it can also be used for LC/MS separation with ammonia-containing mobile phases!

The "Poroshell 300" is an ideal LC column for the analysis of biopharmaceuticals, designed for the rapid separation of proteins and peptides. This column, featuring unique particles, maintains sharp and efficient peak shapes even at high flow rates. With a unique particle structure that combines a porous silica surface thin phase and a hard silica core, it shortens the diffusion distance of proteins, enabling practical high-speed HPLC separations for peptides and proteins up to 500–1,000 kDa. 【Features】 ■ The Poroshell 300 column, bonded with StableBond phases (C18, C8, C3), exhibits excellent stability and sensitivity in TFA and formic acid mobile phases. ■ Usable at pH 2 to 11, it achieves unique separations and high selectivity. *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX 300 StableBond"

Column "ZORBAX 300 StableBond"

In LC/MS analysis in low pH regions, formic acid and acetic acid can also be used as additives!

The "ZORBAX 300StableBond" is an ideal LC column for the analysis of biopharmaceuticals, designed to reproducibly separate proteins and peptides. To efficiently separate large molecules such as proteins and peptides, the sample must easily penetrate the stationary phase surface, which requires a wide pore (300Å) column. This product demonstrates excellent durability against the use of TFA-containing mobile phases in the low pH range typically used for protein and peptide separations. 【Features】 ■ Ability to optimize selectivity for peptides and proteins by utilizing four different stationary phases ■ Provides stability and longevity even when using TFA mobile phases at low pH ■ Excellent reproducibility ■ Offers capillary columns and nano columns suitable for LC/MS applications such as proteomics *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Ion Exchange Column

ZORBAX Ion Exchange Column

Corresponding to organic solvent mobile phases! A lineup including ZORBAX SAX and 300SCX columns.

We would like to introduce the 'ZORBAX Ion Exchange Columns' for the analysis of biopharmaceuticals that we handle. The ZORBAX strong ion exchange columns come in two types: strong anion exchange (SAX) columns and strong cation exchange (300SCX) columns. Both columns are packed with 5μm silica particles, providing suitable analytical efficiency. 【Features】 ■ Stable from pH 2 to 7 ■ Achieves high efficiency and fast separation ■ Compatible with organic solvent mobile phases *For more details, please refer to the related links or feel free to contact us.

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ProSEC 300S

ProSEC 300S

The filler has a nominal pore size of 300 Å! It is suitable for the analysis of a wide range of spherical proteins.

The "ProSEC 300S" is a silica-based column specifically designed for analyzing proteins using size exclusion chromatography. By removing acidic silanol and reducing interaction effects through a unique surface modification, it minimizes non-size exclusion effects across various types of proteins. With a particle size of 5μm, it offers high efficiency and improved separation capability, making it suitable for all protein analyses using salt buffer solutions. 【Features】 ■ Uses robust silica particles with no bleed during use ■ A single column with a wide linear separation range ■ Column dimensions compatible with multi-detector systems *For more details, please refer to the related links or feel free to contact us.

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PL-SAX and PL-WAX for biomolecules

PL-SAX and PL-WAX for biomolecules

LC columns for biopharmaceutical analysis! Polymers are macro-porous spherical styrene-divinylbenzene.

We would like to introduce our products, "PL-SAX & PL-WAX for Biomolecules." "PL-SAX" is a column used in hydrophilic strong anion exchange chromatography. Additionally, due to its high physical stability, it can also be applied for high-speed separations using high flow rates and rapid gradients. Thanks to this excellent stability, the column can be equilibrated quickly, and it is also possible to clean the column with organic solvents, strong acids/strong bases, and high salt concentration solutions. 【Features (Specifications)】 ■ Polymer: Macroporous spherical styrene-divinylbenzene ■ Particle sizes: 5μm, 8μm, 10μm, 30μm ■ Pore sizes: 1000Å, 4000Å ■ Bonding functional groups: Trimethylamino groups ■ Concentration of organic solvents used: 0 to 100 *For more details, please refer to the related links or feel free to contact us.

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PLRP-S for biomolecules

PLRP-S for biomolecules

Chemically and physically stable and robust! LC column for biopharmaceutical analysis.

"PLRP-S for Biomolecules" is an HPLC column filled with macroporous styrene-divinylbenzene. It is a highly reproducible reverse-phase HPLC column, and there is no need for the attachment of alkyl chains (such as C18 or C8) to enhance hydrophobicity. Additionally, the surface of PLRP-S has no unnecessary activity, so there is no occurrence of separation inconsistency due to residual silanol or end-capping rates seen in silica-based fillers. 【Features (Specifications)】 ■ Polymer: Macroporous spherical styrene-divinylbenzene ■ Particle sizes: 3μm, 5μm, 8μm, 10μm, 30μm ■ Pore sizes: 100Å, 300Å, 1000Å, 4000Å ■ Functional groups: None ■ Usable organic solvent concentration: 1–100% (100% water is not allowed) *For more details, please refer to the related links or feel free to contact us.

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PLRP-S for bioanalysis

PLRP-S for bioanalysis

We offer a wide range of pore sizes and particle sizes! All have the same chemistry and fundamental adsorption characteristics.

We would like to introduce our LC column for the analysis of biopharmaceuticals, the 'PLRP-S for Bioanalysis'. Due to the hydrophobic nature of the polymer particles, there is no need for a bonded phase or alkyl bonding groups for separation in reverse phase systems. Therefore, residual silanol and heavy metal contaminants are absent, which does not affect the analysis results. We have suitable columns for separation and preparative purification for analytical purposes, and we can also provide process columns filled with bulk packing materials. 【Features】 ■ Thermally and chemically stable ■ Compliant with USPL21 ■ Pore sizes (100Å to 4000Å) suitable for separating complexes from low to high molecular weights and polynucleotides ■ Contains durable and robust polymer particles that provide highly reproducible results and a long column lifespan *For more details, please refer to the related links or feel free to contact us.

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VariTide RPC

VariTide RPC

Developing a polymer-based HPLC column to economically enhance throughput and analyze and purify synthetic peptides!

We would like to introduce our LC column for biopharmaceutical analysis, 'VariTide RPC.' To efficiently retain peptides composed of 5 to 60 amino acids, we adjust the particle size and pore size. It can be used at various pH ranges, allowing for the separation and purification of acidic, basic, hydrophilic, and hydrophobic peptides with this product. 【Features (Specifications)】 ■ Polymer: Macroporous spherical styrene-divinylbenzene ■ Particle size: 6μm ■ Pore diameter: 200Å ■ Functional groups: None ■ Column size: Inner diameter: 4.6mm to 21.2mm / Length: 25cm *For more details, please refer to the related links or feel free to contact us.

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Biomonolith

Biomonolith

Designed for the separation of macromolecular biomolecules! Polymer-based monolith HPLC column.

We would like to introduce our bio-pharmaceutical analysis LC column, "Bio Monolith." It enables high-speed, high-resolution separation of antibodies (IgG, IgM), plasmid DNA, viruses, phages, and other macro biomolecules. Strong cation exchange phases, strong and weak anion exchange phases, and Protein A phases are available. It is compatible with HPLC and preparative LC systems such as the "1200 Infinity Series," as well as HPLC systems from other manufacturers. 【Features】 ■ Separation unaffected by flow rate ■ High-speed movement between the mobile phase and stationary phase ■ Ultra-fast separation ■ Accelerates method development and reduces costs by fixing method parameters *For more details, please refer to the related links or feel free to contact us.

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Affinity removal column for HSA use

Affinity removal column for HSA use

High-speed single injection protocol and optimized buffer achieve favorable sample recovery rates!

We would like to introduce our "Affinity Removal Column for HSA." This column is designed to remove a large amount of interfering proteins (such as albumin) from human samples (plasma, serum, urine, or cerebrospinal fluid) using chromatography. By removing albumin, the dynamic range of the analysis is effectively expanded, which subsequently improves the sensitivity of sample LC/MS analysis and electrophoresis. 【Features】 - The injection volume of serum per analysis is 130μL for a column with an inner diameter of 4.6mm. - By using a multiple affinity removal system, approximately 50-55% of total proteins (albumin) can be removed. - With a column of 4.6 mm in diameter, up to 26,000μL of plasma/serum can be processed until the column's lifespan is reached. *For more details, please refer to the related links or feel free to contact us.

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Column "DB-WAX Ultra Inert"

Column "DB-WAX Ultra Inert"

Highly active and analytically challenging compounds, minimizing adsorption and decomposition! Achieving consistent inertness.

The "DB-WAX Ultra Inert" column provides symmetrical peak shapes and demonstrates better results for challenging polar compounds by using a highly inert polyethylene glycol. Individually tested with the Ultra Inert WAX test mixture based on QC specifications, it minimizes adsorption and decomposition for highly active, difficult-to-analyze compounds such as free fatty acids, alcohols, diols, and aldehydes, achieving consistent inertness. Additionally, it has the same selectivity as DB-WAX, allowing for easy column upgrades without the need to recreate existing compound libraries. 【Features】 ■ Improved peak shapes for challenging polar compounds ■ High inertness ■ Selectivity equivalent to DB-WAX: no need to revalidate methods ■ Equivalent to USP G16 ■ Excellent performance for acidic compounds including free fatty acids ■ Solvent-cleanable *For more details, please refer to the PDF document or feel free to contact us.

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Column "GC FATWAX Ultra Inert"

Column "GC FATWAX Ultra Inert"

Excellent inter-column inertness performance! Improvement of peak shapes for polar compounds that are difficult to analyze, such as free fatty acids.

The "GC FATWAX Ultranart" is a PEG-type GC column specialized for applications that separate fatty acid methyl esters, fatty acid ethyl esters, and fatty acids. To ensure reproducibility of FAME equivalent chain length values, proper identification of key FAMEs such as EPA, DPA, and DHA, and separation capability of major FAME pairs, tests using FAME mixtures have been conducted. Please feel free to consult us when you need assistance. 【Features】 ■ Column specialized for applications involving FAME, FAEE, and fatty acids ■ Individually tested with FAME mixtures and Ultranart Wax test mixtures ■ Excellent inertness between columns ■ Improved peak shape for polar compounds that are difficult to analyze, such as free fatty acids ■ High polarity, equivalent to USP G16 ■ The chemically bonded and cross-linked stationary phase can be cleaned with solvents and is compatible with water-soluble sample injections *For more details, please refer to the PDF document or feel free to contact us.

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Biopharmaceutical analysis LC column "PL-SAX for biomolecules"

Biopharmaceutical analysis LC column "PL-SAX for biomolecules"

Used in strong anion exchange chromatography with hydrophilicity! Rapidly perform column equilibration.

The "PL-SAX for Biomolecules" is an LC column used for the analysis of biopharmaceuticals in strong anion exchange chromatography with high hydrophilicity. By combining a robust macroporous styrene-divinylbenzene similar to PLRP-S with a polyethyleneimine coating that incorporates trimethylamine groups, it allows for the use of a wide range of pH and various solvents as the mobile phase necessary for the separation of biomacromolecules. Additionally, due to its high physical stability, it is suitable for high-speed separations using high flow rates and steep gradients. 【Features】 ■ Allows the use of a wide range of pH and various solvents as the mobile phase necessary for the separation of biomacromolecules ■ Suitable for high-speed separations using high flow rates and steep gradients ■ Rapid column equilibration ■ Column cleaning with organic solvents, strong acids/strong bases, and high salt concentration solutions is also possible *For more details, please refer to the related links or feel free to contact us.

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