reagent(10) - メーカー・企業と製品の一覧

Long-chain RNA synthesis (mRNA contract synthesis service)

Synthesis of long-chain RNA using in vitro transcription method! Plasmid clones can also be used as templates.

At Fasmac, we offer "long-chain RNA synthesis." We synthesize RNA through in vitro transcription using T7 polymerase from template DNA (prepared from plasmid clones via PCR or oligo DNA), followed by purification with silica columns. The purity and yield are confirmed through absorbance measurement and electrophoresis. Please contact us with your desired sequences for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. 【RNA Usage Examples】 ■ RNA of the GFP gene sequence was synthesized, and after adding a 5' cap and polyA-tail, it was introduced into cultured cells using the lipofection method. ■ Green fluorescence was observed approximately 5 hours after introduction. *For more details, please refer to the PDF materials or feel free to contact us.

企業：ファスマック
価格：Other

Contract Analysis
Contract Analysis
Analysis Services

ブックマークに追加いたしました

ブックマーク一覧

ブックマークを削除いたしました

ブックマーク一覧

これ以上ブックマークできません

会員登録すると、ブックマークできる件数が増えて、ラベルをつけて整理することもできます

無料会員登録

[Case Study] On the Use of PNA in Amplicon Sample Preparation

It is possible to estimate higher resolution feed organisms! Introduction of next-generation sequencing analysis examples *Analysis case collection is being distributed.

In our company, when preparing amplicon samples aimed at analyzing the microbial community structure present in plant leaves, we add PNA to suppress amplification from plant mitochondrial and chloroplast origins, resulting in favorable outcomes. Without the addition of PNA, approximately 90% of the acquired data consists of amplification products derived from the host. By adding PNA, we can reduce this to about 10%, allowing for a higher resolution estimation of the target organisms. [Results of condition testing with 4 sample units] ■ Ct1-Ct4: No PNA added ■ Mt1-Mt4: PNA added for mitochondrial blocking ■ Pl1-Pl4: PNA added for chloroplast blocking ■ PM1-PM4: PNA added for both mitochondrial and chloroplast blocking *For more details, please refer to the PDF document or feel free to contact us.

企業：ファスマック
価格：Other

Contract Analysis

ブックマークに追加いたしました

ブックマーク一覧

ブックマークを削除いたしました

ブックマーク一覧

これ以上ブックマークできません

会員登録すると、ブックマークできる件数が増えて、ラベルをつけて整理することもできます

無料会員登録

GenCheckⓇ qPCR Probe Master

No need for complicated reagent preparation! 2× premix type reagents for real-time PCR.

This product is a 2× premix type reagent for real-time PCR. It contains heat-stable DNA polymerase, Mg2+, Passive Reference Dye, and optimized buffers necessary for real-time PCR (fluorescent probe method), allowing it to be used simply by adding template DNA, primer pairs, and probes. The heat-stable DNA polymerase included in this product is prepared for hot start PCR and is activated by a heat treatment of 95°C for 10 minutes. 【Features】 ■ Enables highly specific real-time PCR ■ No complicated reagent preparation required ■ Compatible with various real-time PCR devices (plate type) ■ Passive reference dye is pre-added ■ By adding UNG (Uracil-N-Glycosylase) separately, carryover prevention treatment is possible (UNG is not included in this product) *For more details, please refer to the PDF document or feel free to contact us.

企業：ファスマック
価格：Less than 10,000 yen

others

ブックマークに追加いたしました

ブックマーク一覧

ブックマークを削除いたしました

ブックマーク一覧

これ以上ブックマークできません

会員登録すると、ブックマークできる件数が増えて、ラベルをつけて整理することもできます

無料会員登録

reagent(10) - メーカー・企業と製品の一覧

reagentの製品一覧

Long-chain RNA synthesis (mRNA contract synthesis service)

[Case Study] On the Use of PNA in Amplicon Sample Preparation

GenCheckⓇ qPCR Probe Master

reagentに関連する検索キーワード