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reagent Product List

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Contract manufacturing service for research reagents utilizing technology cultivated from years of experience.

Leave the contract manufacturing of research reagents to us!

Our company develops and manufactures reagents for genetic engineering research. Leveraging over 38 years of accumulated manufacturing know-how, we respond meticulously to customer requests. Furthermore, we can accommodate optimization of manufacturing processes and quality, as well as small-scale production. 【Features】 ■ Small-scale production available ■ Supply of products that have passed quality testing *For more details, please download the PDF or contact us.

  • Other contract services

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20 x SSC

We offer two types: a 500ml capacity in a glass bottle and a 5L capacity in a polyethylene container!

The product "20 x SSC" consists of 0.3M sodium citrate and 3M sodium chloride. We offer two types of packaging: a 500ml glass bottle and a 5L polyethylene container. Nippon Gene has a wide range of ready-to-use nuclease-free buffer products available. 【Product Details (Partial)】 <20 x SSC (500ml)> ■ Volume: 500ml x 1 ■ Storage: Room temperature ■ Container: Medium bottle *For more details, please refer to the related links or feel free to contact us.

  • Other research reagents

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TE Saturated Phenol

Available in 50ml and 250ml! Contains 0.1% (w/v) 8-hydroxyquinoline.

"TE Saturated Phenol" is used for extracting nucleic acids from biological tissues and for protein removal after nucleic acid enzymatic reactions. It is pre-saturated (equilibrated) with TE buffer [10 mmol/l Tris-HCl (pH 8.0), 1 mmol/l EDTA]. Additionally, this product has a two-layer structure, with the yellow lower layer being TE saturated phenol and the colorless upper layer serving as a TE layer for oxidation prevention. [Features] - Contains 0.1% (w/v) 8-hydroxyquinoline - Composition: 66% phenol, 0.1% 8-quinolinol, saturated with TE - pH: TE saturated phenol 7.2–8.2 *For more details, please refer to the related links or feel free to contact us.

  • Other research reagents

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Nucleic acid staining reagent "EtBr Solution"

Easily captured with a dedicated detection device, and can be pre-dyed! A product in which 10mg of ethidium bromide is dissolved in 1ml of purified sterilized water.

We would like to introduce our nucleic acid staining reagent, 'EtBr Solution'. This product is made by dissolving 10mg of ethidium bromide in 1ml of purified sterilized water. There is no need for the precise weighing or dissolving of hazardous ethidium bromide powder, making it convenient and safe. 【Specifications (partial)】 ■ Appearance: Red solution ■ Concentration: 10mg/ml ■ Measurement wavelength ・λ ex = 254nm (second peak) or 518nm ・λ em = 605nm *For more details, please refer to the related links or feel free to contact us.

  • reagent

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Nucleic acid staining reagent "CLEAR STAIN Blue"

Visible under natural light! Introducing a reagent that stains nucleic acids blue for visualization.

"CLEAR STAIN Blue" is a reagent for staining nucleic acids after agarose gel electrophoresis. This product stains nucleic acids blue for visualization, eliminating the need for detection equipment. Additionally, it has no mutagenicity like ethidium bromide (EtBr), making it very safe and easy to handle. 【Features】 ■ Detection sensitivity is comparable to EtBr ■ Non-mutagenic and very safe ■ No specialized detection equipment required ■ Visible under natural light ■ Can be excised from the gel *For more details, please refer to the related links or feel free to contact us.

  • reagent

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PCR reagent "Gene RED PCR Mix Plus"

Optimized buffer composition to accommodate high-speed PCR! Standard PCR enzyme for research use.

"Gene RED PCR Mix Plus" is a 2x premix type PCR reagent. It contains Taq DNA Polymerase, dNTPs, Mg2+, and other components necessary for PCR, allowing you to perform PCR by simply adding template DNA and primers. Additionally, the buffer composition has been optimized to accommodate fast PCR, making it possible to amplify relatively GC-rich sequences and long fragments (up to 10kb). The resulting PCR products can be used for TA cloning, making it suitable for a wide range of experiments. 【Features】 ■ Easy-to-use 2x premix type ■ Capable of fast reactions (extension time of 10 seconds/kb) ■ Contains two colors of dye, ready to apply as is ■ Compatible with various samples *For more details, please refer to the related links or feel free to contact us.

  • reagent
  • enzyme
  • Other research reagents

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Cas3 protein NLS

Cas3 protein for CRISPR-Cas3 genome editing, which has a nuclear localization signal (NLS).

This product is a Cas3 protein derived from Escherichia coli. It is a protein expressed using insect cells infected with a recombinant baculovirus containing the Cas3 gene, and it has undergone affinity purification. It possesses a nuclear localization signal (NLS) and can be utilized for CRISPR-Cas3 genome editing when combined with the Cascade-crRNA complex. 【Features】 - High concentration product at 15 µg/µL - Contains a nuclear localization signal (NLS) - Enables large-scale deletions in the genome when combined with the Cascade-crRNA complex *For more details, please refer to the catalog.

  • Other bio-related products

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Isothermal nucleic acid amplification reagent "GENEMAL LAMP FL Mix"

Just add the primer and template DNA! Nucleic acid amplification reagent (2× master mix) optimized for the LAMP method.

"GENEMAL LAMP FL Mix" is a master mix for isothermal nucleic acid amplification using the LAMP method. It contains heat-resistant strand-displacing DNA polymerase, Mg2+, dNTPs, and optimized buffers necessary for the LAMP method, allowing for DNA amplification simply by adding primers and template nucleic acid to LAMP FL Mix (2×). This product includes an intercalator (a fluorescent dye that binds to double-stranded DNA) and heat-resistant pyrophosphatase, enabling the detection of amplified DNA using a fluorescence detection device*. <Features> ■ High specificity ■ Gene detection possible in just a few minutes ■ Simply add primers and template nucleic acid ■ Optimized for the ultra-lightweight and compact fluorescence detection device "GENEMAL" *Can be used with the fluorescence detection device "GENEMAL" for LAMP method or real-time PCR devices. (Note) Cannot be detected with turbidity measurement devices. This product is a reagent for research purposes. *For more details, please refer to the PDF document or feel free to contact us.

  • Other Amplification/PCR

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