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reagent Product List

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DNA/RNA extraction reagent from plant samples 'ChloroPure'

DNA/RNA extraction reagent from plant samples 'ChloroPure'

DNA/RNA extraction from plant samples such as fresh leaves, freeze-dried leaves, and powdered seeds using SPRI magnetic beads.

ChloroPure is a kit for simultaneously extracting and purifying DNA/RNA or total RNA only from plant samples (fresh leaves, freeze-dried leaf punches, powdered seeds, etc.). Plants contain a variety of polysaccharides and secondary metabolites, which make nucleic acid extraction challenging; however, ChloroPure enables the extraction of 7 μg of nucleic acids from a sample of 40 mg (or three 6 mm punches of freeze-dried leaves). It is compatible with the automation of Beckman Coulter's Biomek series automated pipetting systems, achieving high-throughput purification of 96 samples in 45 minutes after the centrifugation step following the lysis process. - Simultaneous extraction of DNA/RNA or RNA from 40 mg of plant leaves or seeds - Stable extraction is possible from leaves and seeds of cotton, sunflower, canola, and more *For more details, please refer to the PDF document or feel free to contact us.

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CleanSEQ purification reagent after Sanger sequencing reaction.

CleanSEQ purification reagent after Sanger sequencing reaction.

Purification after Sanger sequencing reaction

CleanSEQ is a fluorescent dye terminator removal purification kit used after Sanger sequencing reactions. Sanger sequencing reaction kits are expensive and are generally used in a diluted form. However, dilution can lead to reduced fluorescence intensity, resulting in decreased sequence read lengths. CleanSEQ allows for high purification yields, which can enhance the signal strength during sequencing. - Efficient removal of unreacted fluorescent dyes and primers - Increased fluorescent signal strength and improved sequence read lengths *For more details, please refer to the PDF document or feel free to contact us.

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Reagent for DNA size selection "SPRIselect"

Reagent for DNA size selection "SPRIselect"

DNA fragment size selection reagent kit

SPRIselect is a kit that allows for the easy recovery of DNA fragments of specific lengths ranging from 150 to 800 bp without the need for cutting agarose gels. By adjusting the mixing ratio of the DNA solution and the SPRIselect reagent, it utilizes the property that the size of DNA fragments binding to magnetic beads can be varied, allowing for the selective recovery of desired fragment sizes. The unique purification protocol using SPRI magnetic beads is optimized, completing manual experiments for double size selection in 20 minutes for 8 samples and 25 minutes for 24 samples. It is also compatible with automation using Beckman Coulter's Biomek series automated dispensing system, achieving high-throughput processing of 48 samples in 51 minutes and 96 samples in 1 hour. - Selectively recover DNA fragments of arbitrary sizes from 150 to 800 bp - High reproducibility achieved through stringent quality control *For more details, please refer to the PDF document or feel free to contact us.

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RNA extraction reagent for cultured cells 'RNAdvance Cell v2'

RNA extraction reagent for cultured cells 'RNAdvance Cell v2'

Reagent kit for RNA extraction from cultured cells using SPRI magnetic beads.

RNAdvance Cell v2 is a kit for extracting and purifying total RNA with minimal degradation from cultured cells (both suspension and adherent cells) of fewer than 50,000 cells. It demonstrates a high purification yield of 1.8 μg of total RNA from 50,000 MRC-5 cells, and similarly high yields from cells such as ACHN, HCT116, and PANC1. Furthermore, the purified RNA has a low degree of degradation (RIN>9.4; RNA Integrity Number), and PCR amplification after reverse transcription is also good, making it suitable for various downstream analyses. It is compatible with the automated dispensing system Biomek series from Beckman Coulter, achieving high-throughput purification of 96 samples in 3 hours. If DNA removal is required, DNase I is needed separately. - RNA extraction from 50,000 cultured cells - Supports both DNA/RNA extraction from cultured cells *For more details, please refer to the PDF document or feel free to contact us.*

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