ネッパジーン List of Products and Services

●Significant reduction in running costs ・No need for liquid nitrogen or freezing agents due to the Stirling cooling method ●Accurate temperature control and temperature profiling ・High performance with a cooling rate of -0.1 to -3℃/min achieved with high reproducibility ・Temperature monitoring and data management possible with sample plate temperature measurement function ・Continuous operation supported by a heating function that returns the plate to the initial set temperature ●Excellent design ・Compatible with CPC, GMP, and isolators ・Lightweight and compact for portability, usable in vehicles 　※Power can be drawn from a cigarette socket (optional) ・Low vibration, low noise, energy-saving design ●Supports various validations ・Compliant with GMP and FDA 21 CFR part 11

• Other frozen and refrigerated storage

● Cells in the body are constantly subjected to various (periodic and intermittent) mechanical stimuli. In contrast, such stimuli are absent in conventional static culture (In Vitro). As a result, cultured cells in static conditions differ from cells in the body (In Vivo) in many ways. The cultured cell stretching device is a system that allows cells to be cultured in conditions closer to the in vivo environment by applying periodic uniaxial (or biaxial*) stretching and compression stimuli to the cultured cells. ● It can be used for research on various adhesive cells such as endothelial, smooth muscle, epithelial, cardiac, adipose, skeletal muscle, fibroblast, osteoblast, and cartilage, as well as for studies related to regenerative medicine. ● It can set up 64 different stimulation patterns with 8 combinations of stretching and compression rates and 8 combinations of stimulation frequencies. ● The system has excellent expandability, and when used for large-scale culture, it can be applied to biochemical experiments at the gene and protein levels. ● The ultra-thin silicone membrane chamber allows for various fluorescence imaging observations.

• Other Physics and Chemistry Equipment

● The main body is made of cell-safe Teflon and PEEK. ● With a variety of options such as perfusion and laminar flow types, open-top types, circular and rectangular shapes, and double-sided slide glass types, it accommodates all applications. ● Autoclavable! Reusable and economical. ● Equipped with an upper observation window, allowing for differential interference imaging.

• Other Physics and Chemistry Equipment

● Glass capillaries for microinjection - Glass needles with a core for microinjection with a tip diameter of 0.5μm or less can be easily produced. - Glass needles with a short tip and a strong body can be manufactured. - DNA, RNA, proteins, and drugs can be injected directly into plant, animal, and yeast cells at low pressure. ● Glass chips for single-cell transfer - Glass chips with a tip diameter of 10μm or more can be easily produced.

• Other Physics and Chemistry Equipment

The LTM-1000 laser thermal expansion micro-injector utilizes the thermal expansion of a laser absorber to inject DNA, RNA, proteins, antibodies, and other substances into the organelles of yeast and plant cells using a glass capillary with an outer diameter range of 0.1 to 0.5 μm. Since injection is possible with a capillary tip diameter of 0.1 μm, there is very little cell damage. Continuous injection is possible.

• Other Physics and Chemistry Equipment

The Sonopole 4000 is a wide-area high-frequency transmitter and amplifier with excellent operability, designed for sonoporation, a non-invasive method for introducing genes and drugs into cells and tissues. The output frequency, output wattage, pulse duty ratio, burst rate (repetition cycle), and sweep function (automatic frequency variation) are continuously variable, allowing for the setting of various combinations of experimental conditions. Automatic measurement of the actual output wattage is possible, and it is displayed in real-time, enabling confirmation of the output wattage during experiments. The probe is a flexible type that can be replaced, and the ultrasonic transducer at the tip is compact, allowing irradiation of areas that were previously difficult to target, thus expanding experimental possibilities. The combination with microbubbles can enhance gene delivery efficiency. It can be used for both In Vivo and In Vitro experiments with a single unit. Please contact us for custom probes.

• Other Physics and Chemistry Equipment

● High spatial resolution: minimum 30μm at 50MHz ● Covers major display modes: Doppler, B, 3D imaging, M, contrast, pulse wave Doppler, analog Doppler, elastic imaging ● Molecular imaging of perfusion information and target information ● Accurate measurement tools for 2D and 3D images ● Capable of connecting with external devices such as lasers and ultrasound transducers, allowing for extended support including photoacoustic imaging, sonoporation, and elastic imaging

• Other Physics and Chemistry Equipment

"CASY" is an international gold standard device that measures cell count and viability with extreme accuracy, non-invasively, without the use of dyes such as trypan blue. In addition to a wide range of applications, high performance, and easy maintenance, it also features excellent reproducibility and durability, fast processing capabilities, and low running costs. The following measurements can be performed in just a few seconds: ● Cell Count: Total cell count and viable cell count ● Cell Viability: Non-invasive measurement without the use of dyes like trypan blue ● Cell Size Distribution: High-resolution (standard below 0.1μm) with a wide dynamic measurement range ● Cell Volume: Very accurate direct volume measurement ● Cell Aggregation: Analysis of cell aggregation factors with automatic correction possible ● Cell Debris: Quality control of cell solutions 【Features】 ■ The sensor is directly immersed in the sample solution ■ Measurement of cell count and viability without staining and non-invasively ■ Wide dynamic measurement range ■ High resolution (standard below 0.1μm) size distribution (over 400 size channels available) ■ Aggregation factor calculated from particle volume *For more details, please refer to the PDF document or feel free to contact us.*

• Other cells
• Other cell research
• Other Analysis

The "ECIS-Zθ" is a cell analysis device that monitors cell dynamics and morphology in real-time without using labels such as fluorescent markers. The operation is very simple; after placing the well in the incubator and pressing the start button, data is automatically collected until the end of the experiment. Using a dedicated well with electrodes, it measures changes in the impedance value (electrical resistance) of the cells. It is possible to non-invasively monitor cell events over a short period to more than 100 hours. In addition to impedance measurement, it is also possible to measure the components of impedance, namely resistance and capacitance. 【Features】 ■ A new assay based on continuous measurement data that cannot be obtained by endpoint methods ■ Measurement can be performed without using labels, reducing cell damage ■ Highly extensible, allowing for experiments such as perfusion culture, hypoxic culture, and imaging *For more details, please refer to the PDF document or feel free to contact us.

• Other cells
• Other cell research
• Other Analysis

"AcquaStain" is a reagent that stains proteins in gels with high sensitivity in just one step. There is no need for pre-treatment, post-treatment, or de-staining of the gel. After adding this product to the gel post-electrophoresis, bands become visible within seconds. Additionally, unlike staining with methanol, isolated proteins are fully compatible with MS analysis. Being water-based, it does not visibly shrink the gel, is non-toxic and odorless, and can be safely disposed of in the sink. 【Features】 ■ Just apply and wait 10 minutes ■ No washing or de-staining required ■ High sensitivity ■ Non-toxic (water-based) and odorless ■ Fully compatible with MS analysis *For more details, please refer to the PDF document or feel free to contact us.

• Protein Extraction Kit
• Other protein preparation
• Other protein analysis

The "NanoAssemblr" is a platform that utilizes microfluidic technology from Precision NanoSystems to instantly create sophisticated and highly reproducible nanomedicines. With its unique cartridge technology, it uses microfluidics to control the self-assembly of nanoparticles, enabling the development of novel nanomedicines. In addition to increased reproducibility, technology transfer has become easier, allowing for a reduction in development time through seamless scale-up. 【Features】 ■ Capable of producing uniformly controlled nanoparticles in about 1 minute ■ Particle size: 20-80nm, Polydispersity Index (PDI): less than 0.1 ■ High reproducibility ■ Can be scaled up directly for clinical development *For more details, please refer to the PDF document or feel free to contact us.

• Other manufacturing equipment
• Other medical supplies and cosmetic manufacturing materials
• others

The "Sonitron GTS" is a sonoporation device that utilizes ultrasound to easily introduce genes and drugs into cells and tissues. It is non-invasive and characterized by minimal cellular damage. By mixing ultrasound microbubbles, such as Targesphere (TS-601, TS-602), with gene or drug solutions, the introduction efficiency can be significantly enhanced. By attaching a HIFU transducer module, HIFU research is also possible. It can accommodate long usage times and is lightweight and compact for easy portability. 【Features】 ■ Significantly increases introduction efficiency by mixing ultrasound microbubbles ■ Transducers are interchangeable ■ Can be used for both In Vivo and In Vitro experiments with a single device ■ Suitable for long usage times, easy to handle, and highly reliable ■ Lightweight and compact for convenient portability *For more details, please refer to the PDF materials or feel free to contact us.

• Other Nucleic Acid/DNA Research

The "Gene Gun System GDS-80" is a gene delivery device that utilizes aerodynamic technology. It uses supersonic gas generated by the pressure difference between the inside and outside of a gas chamber to accelerate particles coated with genes to speeds of up to 300 meters per second for delivery. With simple operation, it achieves high delivery efficiency, and there is no noise during equipment use. Additionally, there is no need to sign a contract for commercial use of the research, allowing for free utilization. 【Features】 ■ Compatible with both plant and animal cells ■ No consumables, simple and low cost (no rupture disk required) ■ Lightweight and portable, allowing for direct delivery without cutting leaves, etc. ■ For animals, DNA delivery is possible without gold particles ■ No contract required for commercial use *For more details, please refer to the PDF materials or feel free to contact us.

• Other Nucleic Acid/DNA Research

UnipicK is a single-cell and tissue picking device that uses a vacuum method. It is compatible with most inverted microscopes. It allows for the instant collection of cells in their natural state without affecting their viability, making it suitable for various downstream experiments. No advanced technical skills are required for operation; it is easy and intuitive, enabling anyone to use it effectively from the start. You can use the culture dishes, plates, and slides that you typically use. 【Features】 ■ Single-cell and tissue picking device using a vacuum method ■ Compatible with most inverted microscopes ■ Instant collection of cells without affecting viability ■ Use of standard culture dishes, plates, and slides *For more details, please request materials or view the PDF data available for download.

• Human cells

The "Micro Pick and Place System" is a device that allows for the easy suction (pick) of small targets, such as cells, one by one while observing them under a microscope, and then the expulsion and placement (place) of each one in a different location. It is based on the PicoPipet, which incorporates a small pump driven by "electroosmotic flow" in the pipette section, enabling anyone to intuitively operate it without requiring expertise to suck, expel, or stop ultra-small amounts (picoliter scale) of liquid. 【Features】 ■PicoPipet ○ Smooth suction, retention, and expulsion possible with simple dial operation ○ Flow rate range: 1nL-10μL/min ○ Operated with a replaceable glass capillary attached to the tip of the pipette section ○ Can be operated by attaching the pipette section to a micromanipulator (It is also possible to operate it by hand depending on the size of the target) ■Compass ○ Simple optical system ○ Allows for high-contrast three-dimensional observation of transparent objects like cells ○ Designed to be suitable for the use of PicoPipet ○ Enables effective micro pick and place operations *For more details, please refer to the catalog or feel free to contact us.

• Other cell research

"ELEPO21" has achieved several times the introduction efficiency against E. coli, bacteria, yeast, and fungi compared to conventional electroporation methods (exponential method) through its unique 4-step multi-pulse method and natural decay method. Additionally, it has significantly improved introduction efficiency while reducing damage to cells. 【Features】 ■ Pore-creating pulses (high voltage, short duration, multiple times, natural decay method) create fine pores in the cell membrane, reducing damage to cells by setting multiple pulses. ■ Polar switching for the ring pulse can be set. ■ Transfer pulses (low voltage, long duration, multiple times, natural decay method) send genes and drugs into the cells multiple times. ■ Further improvement in introduction efficiency is achieved with polar switching transfer pulses. *For more details, please refer to the catalog or feel free to contact us.

• Other Nucleic Acid/DNA Research

The "ECFG21" allows for the production of monoclonal antibodies with a fusion efficiency that is 3 to 6 times higher than that of the PEG method. Additionally, it is compatible with large-capacity chamber electrodes (compared to the lower model LF201 cell fusion device). It automatically measures and displays the voltage and current values of AC and DC pulses. 【Applications】 ■ Cell Fusion - Fusion of spleen cells and myeloma cells - Tetraploid rescue method (tetraploid embryo complementation) - Fusion of ES/EG cells with thymus cells (lymphocytes) - Fusion of human dendritic cells and human tumor cells - Fusion of pancreatic islet cells and tumor cells - Production of humanized monoclonal antibodies - Plant protoplast fusion - Liposome droplet fusion - Yeast and mold cell fusion - Mushroom mycelium cell fusion ■ Nuclear Transfer - Somatic cell nuclear transfer with enucleated recipient oocytes - Nuclear transfer of fertilized egg nuclei with enucleated recipient oocytes ■ Oocyte Activation - Electrical stimulation after sperm injection *For more details, please refer to the catalog or feel free to contact us.

• Other cell research

This is an original cuvette electrode (electroporation cuvette) from NEPA Gene Co., which can be used with most electroporators, including NEPA21 (In Vitro & In Vivo gene introduction system) and Pro-Vitro-S (In Vitro gene introduction system) from NEPA Gene, BioRad's Gene Pulser series, and BTX's ECM series.

• Other Culture

The Super Electroporator NEPA21 Type II has added a damping rate setting function to its 4-step multi-pulse method, achieving high survival rates and high transfection efficiency even for primary cells, which are said to be difficult for gene introduction, as well as immune and blood cells. Additionally, it does not require expensive dedicated reagents or buffers, making it very economical with minimal running costs. 【Gene introduction is possible for various targets such as the following】 ■ Cultured cells (suspension cells) ■ Adherent cultured cells ■ ex vivo tissues (brain slice sections, pancreatic islets, etc.) ■ in vivo mice and rats (muscle, liver, skin, testes, ovaries, brain, retina, etc.) *For more details, please request materials or view the PDF data available for download.

• Other Culture
• Other cell research
• Expression vector