ファスマック List of Products and Services

At Fasmac, we offer "long-chain RNA synthesis." We synthesize RNA through in vitro transcription using T7 polymerase from template DNA (prepared from plasmid clones via PCR or oligo DNA), followed by purification with silica columns. The purity and yield are confirmed through absorbance measurement and electrophoresis. Please contact us with your desired sequences for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. 【RNA Usage Examples】 ■ RNA of the GFP gene sequence was synthesized, and after adding a 5' cap and polyA-tail, it was introduced into cultured cells using the lipofection method. ■ Green fluorescence was observed approximately 5 hours after introduction. *For more details, please refer to the PDF materials or feel free to contact us.

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At Fasmac, we offer "Antibody Sequence Analysis." By obtaining the variable sequences of antibodies, we support the production of recombinant antibodies. We amplify the variable sequences of mouse IgG genes expressed in hybridomas using the 5' RACE method or RT-PCR, perform DNA cloning in E. coli, and conduct sequence analysis. Additionally, we can accommodate other optional tasks depending on the situation. Please feel free to consult with us. 【Analysis Details】 ■ Provided Material: Hybridoma total RNA 1μg (at least 100ng/μl) or cell pellet 1×10^6 cells ■ Deliverables: Report, sequence analysis data (Data for 10 clones each of H chain and L chain will be reported) ■ Delivery Time: 2 to 3 weeks *For more details, please refer to the PDF document or feel free to contact us.

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At Fasmac, we offer "PCR-NGS analysis." We conduct detailed analysis of the types and frequencies of mutations using next-generation sequencers. The analysis device used is the "Illumina MiSeq," which allows for the simultaneous analysis of one target site (up to 16 samples). For details regarding pricing, delivery times, and more, please feel free to contact us. 【Analysis Details】 ■ Simultaneous analysis of 16 samples for one target site ■ Example: Knockout in cultured cells ■ Analysis device: Illumina MiSeq *For more information, please refer to the PDF document or feel free to contact us.

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At Fasmac, we offer "PCR-Sanger Seq analysis." With Sanger sequencing, we can confirm the presence of single nucleotide substitutions and the number of nucleotide deletions/inserts that cannot be detected by electrophoresis analysis. Samples can include tissue fragments from mice, rats, zebrafish, and medaka, as well as cultured cell pellets, and the deliverables will be sequencing analysis data. Please feel free to contact us when you need our services. 【Analysis Details】 ■ Delivery Time: 1-2 weeks ■ Samples: Tissue fragments from mice, rats, zebrafish, and medaka, cultured cell pellets, etc. ■ Deliverables: Sequencing analysis data ■ Analysis Equipment: Applied Biosystems 3730xl DNA Analyzer *For more details, please refer to the PDF document or feel free to contact us.

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At Fasmac, we offer "PCR-Electrophoresis Analysis." We can detect band shifts (10bp and above) due to base deletions/inserts and perform HMA (heteroduplex mobility assay) analysis. The analysis equipment used is the "PerkinElmer LabChip GX Touch HT," and the samples include tissue fragments from mice, rats, zebrafish, and medaka, as well as cultured cell pellets. Please feel free to contact us when you need our services. 【Analysis Details】 ■ Delivery Time: 1 to 2 weeks ■ Samples: Tissue fragments from mice, rats, zebrafish, and medaka, cultured cell pellets, etc. ■ Deliverables: Electrophoresis images ■ Analysis Equipment: PerkinElmer LabChip GX Touch HT *For more details, please refer to the PDF document or feel free to contact us.

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We would like to introduce a case study of our random integration analysis service using the RAIS method. For a knock-in mouse created using CRISPR/Cas, we attempted to detect the knock-in efficiency and off-target sites by combining the RAIS method with NGS. It was suggested that the donor DNA used for the knock-in was inserted not only into the intended chromosome 9 (chr9) but also into another chromosome. 【Random Integration Analysis Service using the RAIS Method Contents】 ■ Sanger Sequencing Plan ■ Next Generation Sequencing (NGS) Plan *For more details, please refer to the PDF document or feel free to contact us.

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We would like to introduce a case study of our random integration analysis service using the RAIS method. HTLV-1 (Human T-cell Leukemia Virus) primarily infects CD4-positive T cells, and its provirus is randomly inserted into the host genome. Currently, the diagnosis of adult T-cell leukemia requires the detection of leukemogenesis (monoclonal proliferation) of HTLV-1 infected cells using the Southern blot method (HTLV-1 clonality analysis). However, this method has various issues, such as requiring a large amount of DNA (blood), involving a long and complex process, and having insufficient detection sensitivity. The RAIS method is a solution that overcomes all these problems, providing highly sensitive and reproducible data for HTLV-1 clonality analysis. 【Content of Random Integration Analysis Service using RAIS Method】 ■ Sanger Sequencing Plan ■ Next Generation Sequencing (NGS) Plan *For more details, please refer to the PDF document or feel free to contact us.

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At Fasmac, we offer "Next-Generation Sequencing" services. DNA analysis using the Sanger method is a widely adopted technology and is gradually being recognized by the general public in forms such as "genetic diagnosis." Our company aims to make next-generation sequencing more accessible to a larger audience by providing contract services using Illumina's MiSeq, with the mission of establishing it as a widely used technology similar to the Sanger method in the future. 【Lineup】 ■ Amplicon analysis ■ Genome draft analysis ■ Custom services ・Sample preparation ・Run on MiSeq ・Data analysis *For more details, please refer to the PDF materials or feel free to contact us.

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To everyone who is worried about 'having to do genotyping but not having enough time,' leave the labor-intensive tasks to Fasmac. In our 'Genotyping Analysis,' we perform DNA extraction, PCR, and mutation detection. Please send us tissue samples from mice or zebrafish, cultured cell pellets, etc. We will provide a quote based on the number of samples and the analysis you require. Additionally, detection using real-time PCR and digital PCR is also possible. 【Line-up】 ■ PCR-Electrophoresis Analysis: Detection of larger indels and screening using HMA ■ PCR-Sanger Seq Analysis: Detection of small indels and single nucleotide substitutions ■ PCR-NGS Analysis: Detailed analysis of mosaic mutations *For more details, please refer to the PDF document or feel free to contact us.

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The "RAISING method" is a technique for detecting the insertion sites of foreign DNA in the genome. It allows for rapid and reproducible measurement results with a small amount of DNA. It can identify insertion sites of viral genome insertions, viral vector insertions, searches for fusion genes that could be drug discovery targets, and off-target knock-ins in genome editing, among others. 【Features】 - Analysis can be arranged according to the purpose and target. - Multiple insertion sites can be detected in a single analysis. - High reproducibility of results is achieved through proprietary technology. 【Analysis Examples】 - HTLV-1 (Human T-cell Leukemia Virus) Clonality Analysis Leveraging the characteristics of the RAISING method, which can quickly detect with a small amount of DNA, we achieved high detection sensitivity even with a proviral load of 0.032%. - Off-target Detection of Knock-ins in Genome Editing For knock-in mice created using CRISPR/Cas, we conducted detection of knock-in efficiency and off-target sites by combining with NGS. *For details on examples and services, please refer to the materials available for download.

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At Fasmac, we offer "Analysis of Live Microbial Community Structure." The "Community Structure Analysis using the Selective Membrane Permeable Dye EMA" utilizes the fact that EMA modifies DNA derived from dead bacteria, rendering the modified DNA unable to be amplified by PCR, allowing for the selective detection of DNA from live bacteria. Additionally, the "Community Structure Analysis using cDNA from Reverse Transcription of RNA" is a method for identifying actively functioning microorganisms by analyzing RNA in environmental samples. 【Features】 <Community Structure Analysis using Selective Membrane Permeable Dye EMA> ■ Utilizes the modification of dead bacterial DNA by EMA, which prevents PCR amplification of the modified DNA ■ Selectively detects DNA from live bacteria <Community Structure Analysis using cDNA from Reverse Transcription of RNA> ■ Analyzes RNA in environmental samples ■ Identifies actively functioning microorganisms *For more details, please refer to the PDF document or feel free to contact us.

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At Fasmac, we offer "RNA-seq analysis." "RNA-seq" allows for comprehensive quantification of all transcripts expressed within cells by analyzing mRNA using NGS. To quantify the expression levels of all transcripts, RNA, which is the transcript from the sample, is first extracted and fragmented. Then, cDNA libraries are created from the fragmented RNA, followed by sequencing. Additionally, we provide a variety of custom services that allow you to choose the analysis content according to your experimental plan. [Analysis Overview] ■ (Customer) Provide sample information ■ Send samples to us ■ Library preparation ■ Sequence analysis ■ Data analysis *For more details, please refer to the PDF document or feel free to contact us.

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At Fasmac, we offer a "Microbial Typing Service (NGS MLST Analysis Service)." "MLST analysis" is a method of typing bacteria by patterning mutations in multiple gene regions (usually more than 7 regions), providing detailed strain information that cannot be distinguished by 16S rRNA gene analysis. The "MLST analysis" using NGS identifies seven gene regions simultaneously by mapping sequence data obtained from shotgun genome sequencing to the genomes of closely related species, and it is believed to be faster and more accurate compared to traditional methods. We have added microbial typing analysis to Fasmac's next-generation sequencing analysis services. Please feel free to contact us if you would like to make an inquiry. *For more details, please refer to the PDF document or feel free to contact us.

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We would like to introduce Fasmac's "Environmental DNA Analysis." In "DNA Metabarcoding using Next-Generation Sequencers," we utilize DNA barcoding regions to analyze diversity in environmental samples. We also accommodate unique target sequences from our customers. Customers can prepare the library themselves, and we will handle everything from sequencing to analysis. Additionally, we offer "Species-Specific Analysis by Quantitative PCR," which can be used for evaluating the presence or absence of organisms and estimating biomass. 【Features】 < DNA Metabarcoding using Next-Generation Sequencers > ■ Analyzing diversity in environmental samples using DNA barcoding regions ■ Free provision of primers for library preparation ■ Support for unique target sequences from customers ■ Possible to handle everything from DNA extraction to library preparation *For more details, please refer to the PDF document or feel free to contact us.

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The "Genome Craft Series" is a powerful genome editing tool that enables free genetic modification. "Genome Craft Type CT" can be used similarly to sgRNA by utilizing chemically synthesized crRNA and tracrRNA, allowing for the chemical synthesis of sgRNA by splitting a long sgRNA into two parts. We provide suitable tools for achieving high-efficiency knock-in/knock-out in CRISPR/Cas genome editing technology. 【Product Lineup】 ■ Guide RNA ・Genome Craft Type CT ・Genome Craft Type SG ■ Recombinant Cas9 ■ Donor DNA *For more details, please refer to the PDF document or feel free to contact us.

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At Fasmac, we offer "artificial gene synthesis." In addition to our standard pUC series vectors, we can also accommodate cloning into specified vectors provided by you. The synthesized sequence will be introduced into the vector in the form of double-stranded DNA and delivered as a plasmid. We significantly reduce the time and effort our customers spend on complicated tasks such as PCR, cloning, and sequencing analysis. 【Features】 ■ Domestic shipping ensures that product transportation does not take extra time ■ Reliable support system due to domestic manufacturing ■ Prompt and detailed responses from our specialized Japanese staff ■ Free codon optimization allows you to leave the tedious sequence design to us *For more details, please refer to the PDF materials or feel free to contact us.

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We can provide analyses tailored to your needs, such as large-scale screening and detailed analysis of individual samples. We will provide a quote based on the number of samples and the content of the analysis.

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It is possible to determine the insertion sites of foreign DNA randomly integrated into the host genome, including the target location. This can be used to identify insertion sites of viral genome, viral vector, fusion genes that may serve as drug discovery targets, and off-target knock-ins in genome editing. Compared to traditional methods for determining transgene insertion sites such as Tail-PCR, LAM-PCR, and Southern blotting, this approach enables analysis that is quicker, simpler, more sensitive, and lower in cost.

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Our "DNA Sequencing Service" allows for detailed and prompt responses for analysis within the country. We have always prioritized speed in our services, and now we have achieved even shorter delivery times. With consistently high-quality data and quick turnaround, we will further accelerate your research. Experience this sense of speed for yourself. 【Features】 ■ Detailed and prompt responses for domestic analysis ■ All data is visually checked by specialized staff ■ Sequencers are maintained by the manufacturer ■ Re-sequencing is conducted for samples expected to improve data quality *For more details, please refer to the PDF document or feel free to contact us.

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The "Contracted Sequence Analysis Rental Analysis Service" is designed for customers who want to request only electrophoresis when "common equipment is crowded," who are "performing sequence reactions under special conditions," or who want to conduct electrophoresis of sequence reaction products more cheaply and quickly. Additionally, we offer the "Contracted Sequence Analysis Premix2 Shortened Delivery Time," which achieves speed and low cost by having customers prepare samples in each container. 【Features】 ■ Rental Analysis - Accepts requests from 1 run, allowing for differentiation based on sample length - Delivery the next business day after sample receipt (as fast as same day!) ■ Semi-Rental Analysis - Recommended for customers who do not want to perform labor-intensive purifications like ethanol precipitation - Delivery the next business day after sample receipt *For more details, please contact us or download the catalog.

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DNA analysis using the Sanger method is a widely adopted technology, and in recent years, "next-generation sequencers" have been released by various companies as the next generation of the Sanger method. However, we believe that the current situation is far from being able to say that DNA analysis using next-generation sequencers is as accessible as the Sanger method. By offering contract services using Illumina's "MiSeq," our mission is to enable more people to utilize analysis with next-generation sequencers and, in the future, to establish it as a widely used technology like the Sanger method. 【Features】 ■ No need for cloning ■ Data that could not be obtained with the Sanger method can be acquired ■ Completed at a low cost and in a short turnaround time *For details, please request materials or view the PDF data from the download.

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"GENOME CRAFT RNA" is a suitable tool for achieving high-efficiency and simple knock-in/out in CRISPR/Cas genome editing technology. The CRISPR/Cas9 system, which is rapidly spreading as a genome editing technology, requires specific RNA for each target sequence. With "GENOME CRAFT RNA," you can easily achieve genome editing by simply mixing it with your Cas9 and introducing it into cells. Please make use of it to enhance the efficiency of your genome editing work. 【Features】 ○ No need for cumbersome expression vector construction or careful RNA preparation tasks [GENOME CRAFT TYPE SG] ○ Production of sgRNA through enzyme synthesis → sgRNA is synthesized via in vitro transcription reactions [GENOME CRAFT TYPE CT] ○ Production of crRNA and tracrRNA through chemical synthesis → By using chemically synthesized crRNA and tracrRNA, it can be used similarly to sgRNA For more details, please contact us or download the catalog.

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