トランスジェニック List of Products and Services

This is an introduction to "CTC measurement," which allows for the assessment of treatment effects and prognosis after drug administration conducted by our company. Measurements are taken using the "CellSearch System" from Janssen Diagnostics, which is approved by the FDA. It counts the number of circulating cancer cells in peripheral blood and monitors cancer prognosis using a minimally invasive method. 【Features】 ■ Counts the number of circulating cancer cells in peripheral blood ■ Monitors cancer prognosis using a minimally invasive method ■ Measurements are taken using the "CellSearch System" from Janssen Diagnostics, which is approved by the FDA *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

This is an introduction to the contract testing services we offer. We conduct "pharmacological tests," which include evaluations of memory learning abilities and analyses using microdialysis, focusing on the "central nervous system," as well as "safety tests" for pharmaceuticals and medical devices. In addition to contract testing, we also provide various services related to toxicity testing and pharmacological testing. Please feel free to contact us if you have any inquiries. 【Contract Testing (Excerpt)】 ■ Pharmacological Tests ■ Safety Tests ■ Pharmaceutical Clinical Trials ■ Food Clinical Trials *For more details, please refer to the PDF document or feel free to contact us.

• Other Testing Contract

We are engaged in the production of "Rosa26 Knock-in Mice." The Gt (ROSA) 26Sor gene is a genetic locus discovered by Phillip Soriano and others using the gene trap method with the ROSA (reverse orientation splice acceptor) vector. The reason many knock-in mice have been created for this genetic locus is that "transcription from the ROSA26 promoter, while not uniform, is ubiquitous." We provide the production of knock-in mice at the Gt (ROSA) 26 Sor locus using various vector backbones. 【Features】 ■ Has ubiquitous promoter activity and is less susceptible to epigenomic effects ■ Stable expression of the gene is possible even when introducing foreign promoters ■ The phenotype of Rosa26 gene disruption does not appear even in homozygous mice ■ High homologous recombination efficiency in ES cells ■ An inducible expression system using Cre/loxP can also be constructed *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

Our company is engaged in the production of "conditional knockout mice." Conditional knockout, also known as condition-specific gene disruption, is a method that involves creating mice with a gene locus flanked by the Cre recombinase target sequence loxP, which targets a specific gene region. By crossing these mice with mice that express Cre recombinase in a tissue- and time-specific manner, we can induce the disruption of the target gene only in specific tissues and at specific times. In recent years, various Cre-expressing mice have been developed, including those that induce activity through drugs (such as CreER), making conditional knockout a very useful method for analyzing gene function. 【Features】 - Possesses loxP sequences, allowing gene disruption through the expression of Cre recombinase. - Enables the analysis of lethal genes. - Allows for tissue-specific gene disruption. - Enables time-specific gene disruption. - We also accept the production of mice from targeting vectors from KOMP and EUCOMM. *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

Our company is engaged in the production of "conventional knockout mice." Conventional knockout mice, also known as complete knockout mice or simple knockout mice, are distinguished from conditional knockout mice. By replacing the exons of the target gene with a drug-resistant gene, the target gene is disrupted in all cells from the early stages of development. Please provide the name of the target gene. Based on the genetic information, we will propose a suitable plan. [Features] - The exons of the target gene are replaced with a drug-resistant gene. - The target gene is disrupted in all cells. *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

Our company is engaged in the production of "transgenic mice." Transgenic mice are created by purifying DNA fragments of the expression unit that includes a promoter, the gene to be expressed, and a polyA sequence from plasmid DNA, and then microinjecting them into fertilized eggs. From the resulting offspring, tissue samples are collected, and genotyping PCR is performed to investigate the insertion of the injected gene into the genome, thereby identifying the founder mouse (FO). [Features] - Production by injecting the gene expression unit into fertilized eggs of the C57BL/6 strain - Forced expression of the gene in mouse individuals - Specific expression can be achieved by selecting the promoter - Strong expression throughout the body is also possible by using the CAG promoter *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

We are engaged in the production of "knock-in mice." Knock-in refers to a method of introducing foreign genes into the mouse genome and analyzing the function of the introduced gene products. It is possible to design the insertion of foreign genes in such a way that endogenous genes are simultaneously disrupted, to design inducible expression using the Cre/loxP system, and to achieve simultaneous expression of multiple foreign genes. The insertion of reporter genes (such as LacZ, GFP, etc.) to investigate the activity of gene expression control regions in the genome also falls under the production of knock-in mice. The gene-modified mouse production we conduct requires advanced technology. 【Features】 ■ Insertion of foreign genes into the mouse genome ■ Simultaneous disruption of endogenous genes is also possible (knock-in and knockout) ■ Expression of reporter proteins can be controlled by endogenous regulatory regions ■ Inducible expression using the Cre/loxP system is also possible ■ Insertion of desired genes at desired locations in the genome at the nucleotide level *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

This test is a pharmacological efficacy study that evaluates the effects of pharmaceuticals and health foods on brain function using small animals. Using a dietary obesity model in mice, we can analyze anti-obesity effects. Additionally, it is possible to conduct assessments of body weight, serum lipids, cytokines, pathological examinations, and genetic analyses. 【Features】 ■ Evaluation of brain function ■ Analysis of anti-obesity effects *For more details, please refer to the catalog or feel free to contact us.

• Other contract services

We conduct pharmacological efficacy tests related to the central nervous system. We evaluate the effects of pharmaceuticals and health foods on brain function using small animal models. Our evaluations include assessments of memory and learning abilities, evaluations of cerebral infarction and brain edema, as well as assessments using the MPTP-induced Parkinson's model. Based on our extensive experience, we provide consulting services for the construction of test systems tailored to our clients' needs. Please feel free to contact us. 【Features】 ■ Tests related to the central nervous system ■ Evaluations concerning brain function *For more details, please refer to our catalog or feel free to contact us.

• Other Testing Contract
• Other contract services
• Other analysis and evaluation services

This service provides analysis using the Luminex(R)200(TM) and Luminex(R)100/200(TM). It allows for the simultaneous quantification of multiple target proteins from serum, plasma, and culture supernatants. With just a few tens of μL of samples such as serum, plasma, and cell culture supernatants, it is possible to quantify and analyze multiple biomarkers. Furthermore, it is possible to select measurement kits tailored to research purposes, accommodating various biological species such as humans, mice, and rats. 【Features】 ■ Simultaneous quantification of multiple biomarkers ■ Analysis possible with small sample volumes ■ Selection of measurement kits according to research purposes ■ Compatibility with various biological species *For more details, please refer to the catalog or feel free to contact us.

• Other contract services

This service is a contract service for producing high-quality monoclonal antibodies. One of the features of GANP(R) mice is that they have a high mutation rate, which makes it easier to obtain antibodies with high affinity and specificity that can recognize small molecules or differences on molecules. We can accommodate requests such as "I want to establish recognition antibodies at the 1 amino acid level," "I want to detect small molecules," "I want to detect only one molecule belonging to a protein superfamily," and "I want to obtain antibodies with higher affinity than existing antibodies." Additionally, we offer optional services for the preparation of culture supernatants during the establishment process and various application evaluations (such as Western blotting and flow cytometry), so please feel free to consult with us. [Features] ■ High mutation rate ■ Ability to recognize small molecules and differences on molecules ■ Easier to obtain antibodies with high affinity and specificity *For more details, please refer to the catalog or feel free to contact us.

• Research Antibodies

The "CytoScan HD Array Analysis" allows for the rapid analysis of chromosomal variations from micro-regions to the whole genome level. It enables the detection of chromosomal copy number abnormalities across the entire human genome and allows for comparison with reference data, including samples from the International HapMap Project. This product is ideal for those who wish to conduct research on hereditary diseases, perform screening for chromosomal copy number abnormalities, or analyze the correlation between gene discovery and copy number. 【Features】 ■ Rapid analysis of chromosomal variations from micro-regions to the whole genome level ■ Detection of chromosomal copy number abnormalities ■ Comparison with reference data *For more details, please refer to the catalog or feel free to contact us.

• Other analysis and sequence

This product is an array capable of performing gene expression analysis. Probes are designed not only for exon regions but also for junction areas, allowing for the analysis of all coding and non-coding transcripts. It covers over 285,000 full-length transcripts, and using the Transcriptome Analysis Console (TAC) Software, visual and easy-to-understand analysis is possible. This product meets requests such as "I want to analyze not only coding transcripts but also non-coding transcripts" and "I want to try it as a pre-test before next-generation sequencing analysis." 【Features】 ■ Probe design adopted ■ Capable of analyzing all coding and non-coding transcripts ■ Covers full-length transcripts *For more details, please refer to the catalog or feel free to contact us.

• Other analysis and sequence
• Other Analysis
• Genetic Analysis Software

This catalog is a comprehensive guide from a company that engages in the production of gene knockout mice, provides genetic information to research institutions such as universities and pharmaceutical companies, sells reagents, and conducts non-clinical and clinical trial outsourcing, as well as pathology diagnostics. It includes various products and services related to the production of genetically modified mice, such as "Conditional Knockout Mouse Production," and gene analysis-related services like "GeneChip Transcriptome Array (Human, Mouse, Rat) 2.0 Analysis" and "GANP(R) Monoclonal Antibody Production." [Overview of Contents] ■ Mice ■ Gene Analysis Related ■ Proteins ■ CRO ■ Pathology Research Outsourcing, etc. *This catalog is a digest version. Please contact us if you need the full version. *For more details, please refer to the catalog or feel free to contact us.

• Cell-related contract